Stem cells are self-replicating and undifferentiated, meaning their function is not yet cell, tissue, or organ-specific. Due to the unique nature of these cells, research into their biology and function holds great promise for therapeutic applications through replacement or repair of diseased and damaged cells. This reader-friendly manual provides a practical "hands on" guide to the culture of human embryonic and somatic stem cells. By presenting methods for embryonic and adult lines side-by-side, the authors lay out an elegant and unique path to understanding the science of stem cell practice. The authors begin with a broad-based introduction to the field, and also review legal and regulatory issues and patents. Each experimental strategy is presented with an historical introduction, detailed method, discussion of alternative methods, and common pitfalls. This lab guide for researchers also serves as a textbook for undergraduate and graduate students in laboratory courses.
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oligodendrocyte differentiation, stem cell transplantation, human feeder cells, classical cytogenetics, cell dissociation buffer, defined culture systems, cell strainer, cytogenetic nomenclature, feeder cell medium, neural induction medium, neuroepithelial clusters, chromosome harvest, primitive neuroepithelial cells, inactivated feeder cells, cultured human embryonic stem cells, embryo check, human neural progenitor cells, human embryonic stem cell lines, organ culture dish, cat catheter, peel pack, use within two weeks, teratoma composed, human embryonic stem cell research, beating areas Key Phrases - Capitalized Phrases (CAPs): (learn more)
Human Stem Cell Manual, Nat Biotechnol, Fisher Scientific, Component Amount Final, Copyright Elsevier Inc, Item Supplier Catalog, Component Amount Stock, Molecular Probes, Proc Natl Acad Sci, Irvine Scientific, United States, Reprod Biomed Online, Trypan Blue, Jackson Laboratories, Nat Rev Genet, Dev Biol, Neural Progenitor Cell Culture, Cold Spring Harbor, Nat Methods, Sigma Aldrich, Developmental Studies Hybridoma Bank, Neurosci Res, Component Supplier, Hum Reprod, Chemicon International New!
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oligodendrocyte differentiation, stem cell transplantation, human feeder cells, classical cytogenetics, cell dissociation buffer, defined culture systems, cell strainer, cytogenetic nomenclature, feeder cell medium, neural induction medium, neuroepithelial clusters, chromosome harvest, primitive neuroepithelial cells, inactivated feeder cells, cultured human embryonic stem cells, embryo check, human neural progenitor cells, human embryonic stem cell lines, organ culture dish, cat catheter, peel pack, use within two weeks, teratoma composed, human embryonic stem cell research, beating areas Key Phrases - Capitalized Phrases (CAPs): (learn more)
Human Stem Cell Manual, Nat Biotechnol, Fisher Scientific, Component Amount Final, Copyright Elsevier Inc, Item Supplier Catalog, Component Amount Stock, Molecular Probes, Proc Natl Acad Sci, Irvine Scientific, United States, Reprod Biomed Online, Trypan Blue, Jackson Laboratories, Nat Rev Genet, Dev Biol, Neural Progenitor Cell Culture, Cold Spring Harbor, Nat Methods, Sigma Aldrich, Developmental Studies Hybridoma Bank, Neurosci Res, Component Supplier, Hum Reprod, Chemicon International New!
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Front Cover | Table of Contents | First Pages | Index | Surprise Me!
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