In Situ Hybridization Protocols for the Brain (Biological Techniques Series) [Paperback]

W. Wisden (Editor), B. J. Morris (Editor), David B. Sattelle (Series Editor)

Book Description

ISBN-10: 0127599207 | ISBN-13: 978-0127599205 | Publication Date: July 27, 1994

Biological Techniques is a series of volumes aimed at introducing to a wide audience the latest advantages in methodology. The pitfalls and problems of new techniques are given due consideration, as arethose small but vital details not always explicit in the methods sections of journal papers.
In recent years, most biological laboratories have been invaded by computers and a wealth of new DNA technology. This boon in technology will be reflected in many of the titles appearing in the series.
The books will be of value to advanced researchers and graduate students to learn and apply new techniques, and will be useful to teachers of advanced undergraduate courses involving practical or project work.

Editorial Reviews

Review

"In situ Hybridization Protocols for the Brain is aimed at first time users, although the experienced user will also find tons of information and useful know-hows in this book."
--PSYCHONEUROENDOCRINOLOGY 29 (2004) --This text refers to the Hardcover edition.

From the Back Cover

Biological Techniques is a series of volumes aimed at introducing to a wide audience the latest advances in methodology. The pitfalls and problems of new techniques are given due consideration, as are those small but vital details not always explicit in the methods sections of journal papers.
In recent years, most biological laboratories have been invaded by computers and a wealth of new DNA technology and this will be reflected in many of the titles appearing in the series.
The books will be of value to advanced researchers and graduate students seeking to learn and apply new techniques, and will be useful to teachers of advanced undergraduate courses involving practical or project work.
In situ hybridization is an important technique in probing the cellular sites of gene expression within a tissue. It is a particularly valuable tool when applied to the brain, because it allows the specific cell types expressing a given gene to be delineated from the complex mosaic of other cells constituting neuronal tissue.
Although there have been several previous volumes describing this method, this one is aimed at the neurobiologist who would like to set up the method and wants a good simple protocol.
The first part of the book concentrates on a well tried and tested technique, using the radiolabelled-oligonucleotide method.
The second part covers some more recent and less standardised advances in in situ hybridization technology using non-radioactive methods. The writers of this book are convinced that in situ hybridization techniques can be used in any laboratory by anyone. The volume has been designed so that someone without any previous experience of the techniques can follow the recipes and expect to get a good result. --This text refers to the Hardcover edition.

See all Editorial Reviews

Product Details

• Paperback: 144 pages
• Publisher: Academic Press (July 27, 1994)
• Language: English
• ISBN-10: 0127599207
• ISBN-13: 978-0127599205
• Product Dimensions: 10 x 7.8 x 0.5 inches
• Shipping Weight: 12.2 ounces
• Amazon Best Sellers Rank: #6,037,935 in Books (See Top 100 in Books)