In Situ Hybridization Protocols (Methods in Molecular Biology (Cloth)) [Hardcover]

Ian A. Darby (Editor)

Book Description

ISBN-10: 0896036863 | ISBN-13: 978-0896036864 | Publication Date: January 15, 2000 | Edition: 2 Sub

Ian Darby updates the highly successful premier edition with a full panoply of new and greatly augmented state-of-the-art techniques. This valuable new edition contains basic and advanced in situ hybridization techniques - many not covered in the first edition - and includes protocols for in situ hybridization of whole-mount embryo specimens, in situ hybridization at the electron microscope level, in situ detection of DNA fragmentation in apoptosis, localization of genes to particular chromosomes, and the use of DNA or RNA probes to detect expression in cells or tissue sections. Prepared by leading investigators who have daily worked with the techniques, fine-tuning them for reliability, In Situ Hybridization Protocols, 2nd Edition, offers a completely updated and extended collection of proven and readily reproducible methods suitable for both the novice and experienced investigator. Its state-of-the-art protocols constitute the new standard methods resource in the field, one that will enable researchers successfully to enhance and improve their current experimental repertoire.

Editorial Reviews

Review

From Reviews of the First Edition by Andy Choo... "...a very helpful manual...essential for any laboratory that is using or wants to use the in situ hybridization technique in their repertoire." - - Molecular Biotechnology " ...a good book for anyone wishing to exploit all that in situ hybridization has to offer." - - Biopharm Manufacturing Reviews for Second Edition This book should be of interest to anyone using or considering in situ hybridization, as it provides well written, if rather specialized, examples of the many applications of this extremely useful but notoriously tricky technique The text is well referenced and includes useful details of reagent suppliers as well as links to further information available over the internet. This is a book that aims to make in situ hybridization accessible to all, and to inspire new applications for the technique. In my opinion, it does a reasonably sound job of both. - Heredity

From the Back Cover

In In Situ Hybridization, Third Edition, Ian Darby updates his highly successful second edition and shifts the focus to tissue and cell in situ hybridization. Drawing on experts working in diverse areas and taking advantage of advances in non-isotopic in situ hybridization and amplification techniques, the authors describe in detail their readily reproducible techniques for preparation, detection, and tissue hybridization. Among the new techniques detailed are PNA probes for viral diagnostics, plant in situ hybridization, cell proliferation detection, and quantitation of in situ hybridization. There are also cutting-edge techniques for tissue microarrays, expanded embryology-developmental gene detection, and expanded cell culture. Derivative techniques presented include the identification of transplanted cells, histones, nick-end labeling for apoptosis, the use of peptide nucleic acid probes, and in situ hybridization of plant specimens. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Completely updated and highly practical, In Situ Hybridization, Third Edition allows both the molecular biologist with little experience of histology and the histologist with little experience of molecular biology to use these powerful new techniques successfully. --This text refers to the Paperback edition.

Product Details

• Hardcover: 343 pages
• Publisher: Humana Press; 2 Sub edition (January 15, 2000)
• Language: English
• ISBN-10: 0896036863
• ISBN-13: 978-0896036864
• Product Dimensions: 9.5 x 6.3 x 0.9 inches
• Shipping Weight: 1.6 pounds (View shipping rates and policies)
• Average Customer Review: 5.0 out of 5 stars  See all reviews (1 customer review)
• Amazon Best Sellers Rank: #3,747,704 in Books (See Top 100 in Books)

Customer Reviews

Most Helpful Customer Reviews

4 of 4 people found the following review helpful:

5.0 out of 5 stars THE book for in situ methodologies, November 3, 1999

By A Customer

This review is from: In Situ Hybridization Protocols (Methods in Molecular Biology (Cloth)) (Hardcover)

With the rapid and on-going development of In Situ hybridisation, a new edition of this book was needed. In Professor Darby the publishers found a very capable editor. He has gathered articles which, while conveying all the protocols you would need to reproduce the techniques in your own laboratory, are surprisingly readable.

Even for the experienced practitioner, there are methods in here that will catch you by surprise. The wealth of illustrations add to the enjoyment, as you gain a very clear idea of what to expect. It is a shame, however, that most are in black and white. The supplement of colour photos (and the page in the professor's own article) demonstrate the results most clearly.

As the summary says, if you are interested in hybridisation - whether of protein, chromosomes or even whole embryos - this is an indispensable manual.

(caveat: probably not a must-by for the general reader)