The aim of this book is to describe and discuss the preparation of immuno-gold probes, the progress and development of preparation methods suitable for immuno-electron microscopy, and the use of immuno-gold labeling at the microscopic level. The perspectives and limitations of the different methods used in combination with immuno-gold are evaluated not only in the methodology chapters, but also in the chapters where the validity of immuno-gold labeling is exemplified in a successful application to biological questions. In addition to examining cryosections as one of the most common preparation methods for immuno-electron microscopy, an alternative embedding method, such as freeze substitution of rapidly frozen samples, and replica methods, such as freeze-etching, fracture label, label fracture, and whole mount, are discussed in detail. This book is an excellent source for cell biologists, researchers, technicians, and students.
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First Sentence:
Among (immuno)cytochemical marker systems, colloidal gold particles have undergone an enormous evolution since their introduction by Faulk and Taylor. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
antiluciferase antibody, mature trichocysts, metal mirror cryofixation, cytoskeleton buffer, dry cleavage, epoxide embedding, protoplasmic surface, matrix compactness, exoplasmic surface, semithin sectioning, colloidal gold granules, cytochemical marker for light, improved structural preservation, sectioning characteristics, sectioning speed, exoplasmic fracture face, surface replication, scanning electron probe, label efficiency, topographical resolution, substitution media, ultrathin cryosections, ultrathin frozen sections, residual aldehyde groups, labeling density Key Phrases - Capitalized Phrases (CAPs): (learn more)
Cell Biol, Electron Microsc, New York, Academic Press, Science of Biological Specimen Preparation, Cell Res, John Wiley, Cell Motil, Cell Sci, San Francisco Press, Colloid Interface Sci, Janssen Life Sciences Products, Nature Phys, Plenum Press, Practical Methods, The Hague, Advanced Techniques, Cambridge Instruments Company, Cell Physiol, Van Damme-Jongsten New!
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Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Among (immuno)cytochemical marker systems, colloidal gold particles have undergone an enormous evolution since their introduction by Faulk and Taylor. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
antiluciferase antibody, mature trichocysts, metal mirror cryofixation, cytoskeleton buffer, dry cleavage, epoxide embedding, protoplasmic surface, matrix compactness, exoplasmic surface, semithin sectioning, colloidal gold granules, cytochemical marker for light, improved structural preservation, sectioning characteristics, sectioning speed, exoplasmic fracture face, surface replication, scanning electron probe, label efficiency, topographical resolution, substitution media, ultrathin cryosections, ultrathin frozen sections, residual aldehyde groups, labeling density Key Phrases - Capitalized Phrases (CAPs): (learn more)
Cell Biol, Electron Microsc, New York, Academic Press, Science of Biological Specimen Preparation, Cell Res, John Wiley, Cell Motil, Cell Sci, San Francisco Press, Colloid Interface Sci, Janssen Life Sciences Products, Nature Phys, Plenum Press, Practical Methods, The Hague, Advanced Techniques, Cambridge Instruments Company, Cell Physiol, Van Damme-Jongsten New!
Books on Related Topics | Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Citations (learn more)
This book cites 24
books:
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10
books
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- Techniques in Immunocytochemistry, Volume 3 by Gillian R. Bullock on 9 pages
- Immunolabelling for Electron Microscopy by Julia M. Polak on 6 pages
- Cryotechniques in Biological Electron Microscopy by R.A. Steinbrecht on 5 pages
- Practical Methods in Electron Microscopy by A.M. Glauert on page 92, page 133, and page 176
- Advanced Techniques in Biological Electron Microscopy by J. K. Koehler on page 92, and page 214
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- Antibodies in Cell Biology, Volume 37 (Methods in Cell Biology) by David J. Asai on 5 pages
- Immunogold-Silver Staining: Principles, Methods, and Applications by M. A. Hayat on page 116, and page 230
- Biomedical Electron Microscopy: Illustrated Methods and Interpretations by Arvid B. Maunsbach on page 426, and page 454
- Immuno Gold Electron Microscopy in Virus Diagnosis and Research by Alexander D. Hyatt on page 306, and page 430
- Introduction to Biophysical Methods for Protein and Nucleic Acid Research by Jay A. Glasel on page 259, and page 260
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