Immunocytochemical Methods and Protocols (Methods in Molecular Biology) [Hardcover]

Constance Oliver (Editor), Maria Célia Jamur (Editor)

Book Description

ISBN-10: 1588294633 | ISBN-13: 978-1588294630 | Publication Date: December 23, 2009 | Edition: 3rd

The landscape of immunocytochemistry has significantly evolved in past years. Once a laborious technique with limited application, the introduction of new detection systems and instrumentation has facilitated the widespread use of immunocytochemical methods. In the post-genomic era, the use of immunocytochemical methods has grown to include the identification of a broad spectrum of antigens including proteins, carbohydrates and lipids from virtually every organism. In Immunocytochemical Methods and Protocols, Third Edition, expert researchers explore the modern methods employed in the field today,  now widely used to identify, in situ, various components of cells and tissues in both normal and pathological conditions, and to demonstrate the localization of subcellular components. Chapters provide methods that are designed to be general in their application and to appeal to investigators across all disciplines, including those in research and clinical settings. Composed in the highly successful Methods in Molecular Biology™ series format, chapters contain a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.  Comprehensive and ground-breaking,  Immunocytochemical Methods and Protocols, Third Edition, brings together a variety of methods that are normally limited to applications in a specific area, providing a unique and vital guide for researchers and clinicians alike.

Editorial Reviews

Review

This book is a useful, sometimes beautiful, compilation of protocols and methods...-FEBS Letters
...offer[s[ unique features that make [it a] worthwhile purchase...comprehensive-Trends in Genetics
"This second edition provides detailed protocols for immunocytochemical localization of tissue constituents. The book is well balanced and suited for basic and clinical research scientists. . .The editor has successfully compiled this book for the intended readers. The editor and contributors are credible. . .The protocol style format is useful for a first experiment. The illustrations are attractive and informative. The book as a whole is nicely done. . .I find this a very useful and practical addition to all fields of biology and medicine. It is very user friendly and should be a great reference for those designing experiments and writing."-Doody's Health Sciences Book Review Journal
., ."this book would make a valuable addition to laboratories currently involved in research using immunocytochemical techniques and would be particularly useful for those wishing to introduce these techniques into their research programmes...Given the depth of coverage of theory behind various techniques and the detailed notes that accompany each set of protocols, the book would also make an ideal teaching aid."-Molecular Biotechnology
., ."this excellent book on Methods and Protocols can be recommended to both investigators starting out in the field of immunocytochemistry and to basic and clinical researchers already working in the field because it contains a plethora of methods and protocols for the demonstration of molecules in manydifferent normal and pathological situations and also under different experimental conditions." - Journal of Chemical Neuroanatomy

From the Back Cover

The landscape of immunocytochemistry has significantly evolved in past years. Once a laborious technique with limited application, the introduction of new detection systems and instrumentation has facilitated the widespread use of immunocytochemical methods. In the post-genomic era, the use of immunocytochemical methods has grown to include the identification of a broad spectrum of antigens including proteins, carbohydrates and lipids from virtually every organism. In Immunocytochemical Methods and Protocols, Third Edition, expert researchers explore the modern methods employed in the field today,  now widely used to identify, in situ, various components of cells and tissues in both normal and pathological conditions, and to demonstrate the localization of subcellular components. Chapters provide methods that are designed to be general in their application and to appeal to investigators across all disciplines, including those in research and clinical settings. Composed in the highly successful Methods in Molecular Biology™ series format, chapters contain a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.  Comprehensive and ground-breaking,  Immunocytochemical Methods and Protocols, Third Edition, brings together a variety of methods that are normally limited to applications in a specific area, providing a unique and vital guide for researchers and clinicians alike.

Product Details

• Hardcover: 401 pages
• Publisher: Humana Press; 3rd edition (December 23, 2009)
• Language: English
• ISBN-10: 1588294633
• ISBN-13: 978-1588294630
• Product Dimensions: 10.1 x 7 x 1.1 inches
• Shipping Weight: 2.1 pounds (View shipping rates and policies)
• Amazon Best Sellers Rank: #2,305,716 in Books (See Top 100 in Books)