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RNA Isolation and Characterization Protocols (Methods in Molecular Biology)
 
 
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RNA Isolation and Characterization Protocols (Methods in Molecular Biology) [Hardcover]

Ralph Rapley (Editor), David L. Manning (Editor)

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Book Description

0896034941 978-0896034945 April 8, 1998 1
Innovative biological investigators offer a powerful and highly useful collection of up-to-date methods for the isolation of RNA from a variety of sources, including bacterial, plant, and mammalian cells. These fine-tuned protocols take both the experienced and inexperienced investigator through the nuances of RNA manipulation, from extraction to in vitro translation. The book also includes protocols for the analysis and characterization of isolated RNA species and extensive troubleshooting hints and tips to ensure easy reproducibility. RNA Isolation and Characterization Protocols is the single best collection of easily reproducible, step-by-step RNA techniques now available for today's bench scientists.

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"This book is aimed at clinical specialists as well as experimentalists. . .The references are recent. I strongly recommend its use by those involved in breast cancer treatment. It is one of the few books that deal with the management of benign breast conditions."-Oncology

From the Back Cover

RNA Isolation and Characterization Protocols offers biological investigators a powerful and highly useful collection of up-to-date methods for the isolation of RNA from a variety of sources, including bacterial, plant, and mammalian cells. These fine-tuned protocols take both the experienced and inexperienced investigator through the nuances of RNA manipulation, from extraction to in vitro translation. The authoritative researchers writing here also include detailed protocols for the analysis and characterization of isolated RNA species and extensive troubleshooting hints and tips to ensure easy reproducibility.

RNA Isolation and Characterization Protocols gives today's bench scientists the single best collection of easily reproducible, step-by-step RNA techniques now available. This book will quickly become indispensable for everyone seeking to manipulate RNA and help set the stage for the coming revolution in RNA-based diagnostic techniques.

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Inside This Book (learn more)
First Sentence:
Ribonucleic acid (RNA) is an unbranched polymer of purine (adenine, guanine) and pyrimidine (cytosine, uracil) nucleotides joined by phosphodiester bonds. Read the first page
Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
minisequencing analysis, detection step primer, denaturation buffer, protease protection, genome segments, guanidinium thiocyanate, phenol treatment, hybridization buffer, wheat germ extract, translation reaction, reference gene, ribonuclease inhibitor, kinase buffer, autoclaved water, guanidine thiocyanate, translational activity, transcription buffer, substrate buffer, nucleotide mix, transcription reaction, hybridization temperature, deionized formamide, diethyl pyrocarbonate, translation products, radioactive amino acids
Key Phrases - Capitalized Phrases (CAPs): (learn more)
Humana Press Inc, Nucleic Acids Res, Cold Spring Harbor, Methods Enzymol, New York, Laboratory Manual, Materials All, Molecular Cloning, Oak Ridge, Pharmacia Biotech, Plant Mol, Boehringer Mannheim, Practical Approach, Amersham International, Cancer Res, Life Technologies, Perkin Elmer, Preparation of Single-Stranded
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