Highly skilled investigators with extensive experience in gene targeting and mouse genetics describe their best techniques for the design of targeting constructs and for the analysis of the mouse phenotype. These include embryo transplantation, in vitro embryonic stem cell differentiation, creation of aggregation chimeras, mouse pathology, embryo cryopreservation, and transplantation. State-of-the-art and highly practical, Gene Knockout Protocols not only constitutes an invaluable source of readily reproducible techniques for those just entering the field of gene targeting, but also a key reference for all genetic researchers today.
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Book Description
Editorial Reviews
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"This is a valuable reference work, which will no doubt find a home on many lab benches." -E-STREAMS
From the Back Cover
As the major task of sequencing the human genome nears completion and the full complement of human genes are catalogued, the task of understanding the normal biological functions of genes and how their alteration leads to diseased states becomes more imperative. In Gene Knockout Protocols, highly skilled investigators with extensive experience in gene targeting and mouse genetics describe their best techniques for the design of targeting constructs and for genetic phenotype analysis. These proven methods contain step-by-step instructions, as well as notes on pitfalls to avoid, and emphasize techniques that are relevant to researchers carrying out gene targeting work. These include embryo transplantation, in vitro embryonic stem cell differentiation, creation of aggregation chimeras, mouse pathology, embryo cryopreservation, and transplantation. Issues such as the use of existing mouse mutation resources and the influence of genetic background and epigenetic effects upon phenotype are also covered. State-of-the-art and highly practical, Gene Knockout Protocols not only constitutes an invaluable source of readily reproducible techniques for those just entering the field of gene targeting, but also a key reference for all genetic researchers today.
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Inside This Book
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First Sentence:
The ability to generate a mouse with a targeted mutation in a desired gene has been one of the most important advances in understanding the function of gene products. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
conditionally mutant locus, dark neuron artifact, primitive ectoderm derivatives, target gene homology, mutant target locus, hematopoietic phenotypes, primitive erythroid colonies, neural primordia, cell transplantation studies, marker gene cassette, aggregation plate, tetraploid embryos, injection pipet, conditional gene knockouts, cell strainers, mouse pathology, vivo libraries, neural primordium, knockout mouse phenotypes, cranial paraxial mesoderm, microinjection buffer, photometer system, embryoid bodies, targeting frequency, differentiation medium Key Phrases - Capitalized Phrases (CAPs): (learn more)
Nucleic Acids Res, Humana Press Inc, Gene Knockout Protocols Edited, Genes Dev, Cell Biol, New York, Boehringer Mannheim, Academic Press, Pathologic Phenotypes of Knockout Mice, Cold Spring Harbor Laboratory Press, Analysis of Hematopoietic Phenotypes, Fate Mapping of Neural Primordium, Choice of Mouse Strains, Pathology of Laboratory Animals, Trends Genet, Centralized Facility, Epigenetic Effects, Manipulating Mouse, Becton Dickinson, Practical Approach, Transgenic Studies, Cell Res, Laboratory Manual, Isolation of Embryonic Fibroblasts, San Diego New!
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Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
The ability to generate a mouse with a targeted mutation in a desired gene has been one of the most important advances in understanding the function of gene products. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
conditionally mutant locus, dark neuron artifact, primitive ectoderm derivatives, target gene homology, mutant target locus, hematopoietic phenotypes, primitive erythroid colonies, neural primordia, cell transplantation studies, marker gene cassette, aggregation plate, tetraploid embryos, injection pipet, conditional gene knockouts, cell strainers, mouse pathology, vivo libraries, neural primordium, knockout mouse phenotypes, cranial paraxial mesoderm, microinjection buffer, photometer system, embryoid bodies, targeting frequency, differentiation medium Key Phrases - Capitalized Phrases (CAPs): (learn more)
Nucleic Acids Res, Humana Press Inc, Gene Knockout Protocols Edited, Genes Dev, Cell Biol, New York, Boehringer Mannheim, Academic Press, Pathologic Phenotypes of Knockout Mice, Cold Spring Harbor Laboratory Press, Analysis of Hematopoietic Phenotypes, Fate Mapping of Neural Primordium, Choice of Mouse Strains, Pathology of Laboratory Animals, Trends Genet, Centralized Facility, Epigenetic Effects, Manipulating Mouse, Becton Dickinson, Practical Approach, Transgenic Studies, Cell Res, Laboratory Manual, Isolation of Embryonic Fibroblasts, San Diego New!
Books on Related Topics | Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Citations (learn more)
This book cites 74
books:
See all 74 books this book cites
5
books
cite this book:
- Gene Targeting by John M. Sedivy on page 63 (1), page 63 (2), and page 63 (3)
- Recombinant Gene Expression Protocols (Methods in Molecular Biology) by Rocky S. Tuan on page 317, and page 318
- Gene Targeting: A Practical Approach by Alexandra L. Joyner on page 63 (1), and page 63 (2)
- Stem Cells (Handbook of Experimental Pharmacology) by Anna M. Wobus on page 263, and page 284
- Manipulating the mouse embryo: A laboratory manual by Brigid Hogan on page 18, and page 63
See all 74 books this book cites
- Cancer Cell Signaling: Methods and Protocols (Methods in Molecular Biology) by David M. Terrian on page 46
- Quantitative Trait Loci: Methods and Protocols (Methods in Molecular Biology) by Nicola J. Camp in Front Matter
- Transgenesis Techniques: Principles and Protocols (Methods in Molecular Biology) by Alan R. Clarke on page 123
- Mouse Phenotypes: A Handbook of Mutation Analysis (Manual) by Virginia E. Papaioannou in Back Matter
- DNA Methylation Protocols (Methods in Molecular Biology) by Ken I. Mills in Front Matter
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