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Malaria Methods and Protocols (Methods in Molecular Medicine)
 
 
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Malaria Methods and Protocols (Methods in Molecular Medicine) [Hardcover]

Denise L. Doolan (Editor)

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Book Description

July 1, 2002 0896038238 978-0896038233 1st
Internationally respected scientists and clinicians describe in step-by-step detail their most useful conventional and cutting-edge techniques for the study of malaria. Areas covered include clinical and laboratory diagnosis and typing, animal models, molecular biology, immunology, cell biology, vaccinology, laboratory models, and field applications. Each readily reproducible protocol has been tested, standardized, and optimized for experimental success, and includes many laboratory notes on troubleshooting, avoiding pitfalls, and interpreting results. Several of the most widely used methods are described here in detail for the first time or have been thoroughly updated since their original publication (e.g., in vitro culture of Plasmodium parasites and in vitro growth inhibition assay).

Editorial Reviews

Review

"In this book, internationally respected scientists and clinicians describe in step-by-step detail their most useful conventional and cutting-edge techniques for the study of malaria." - Clinical Laboratory International "This is an outstanding collection of protocols written by over eighty experts in the field. The geographical spread of these contributors is impressive, encompassing writers from thirteen countries on six continents. There is good stylistic continuity throughout the book, despite the diversity of the authors...there is admirable cross-referencing between chapters." -Molecular Biotechnology

From the Back Cover

Despite considerable scientific and medical effort over the past decades, malaria remains the most important human parasitic disease. It is responsible for up to 3 million deaths and another 300-500 million new cases each year, and is becoming resistant to the current chemoprophylactic and chemotherapeutic agents. In Malaria Methods and Protocols, internationally respected scientists and clinicians describe in step-by-step detail their most useful conventional and cutting-edge techniques for the study of malaria. Areas covered include clinical and laboratory diagnosis and typing, animal models, molecular biology, immunology, cell biology, vaccinology, laboratory models, and field applications. Each readily reproducible protocol has been tested, standardized, and optimized for experimental success, and includes many laboratory notes on troubleshooting, avoiding pitfalls, and interpreting results. Several of the most widely used methods are either described here in detail for the first time or have been thoroughly updated since their original publication (e.g., in vitro culture of Plasmodium parasites and in vitro growth inhibition assay). State-of-the-art and highly practical, Malaria Methods and Protocols makes available to basic and applied researchers today's only comprehensive collection of essential laboratory methods for diagnosing malaria, characterizing the parasite, understanding the interaction between the human host and Plasmodium parasite, and developing effective preventive measures.

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Inside This Book (learn more)
First Sentence:
This chapter provides standard methods for the incrimination of Anopheles mosquito species serving as malaria vectors and associated methods for measuring the intensity of transmission. Read the first page
Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
final parasitemias, sporozoite suspension, berghei clone, berghei transformation, adhesion buffer, effector cultures, parasite pellet, infective gametocytes, antiadhesion antibodies, mosquito samples, parasite suspension, pyrimethamine selection, weekly medium changes, sporozoite transmission, recombinant locus, initial parasitemias, warming platform, cell strainer, parasitized cells, treatment with chloroquine, adjust cell concentration, sealing mat, chabaudi chabaudi, master mix tube, allelic family
Key Phrases - Capitalized Phrases (CAPs): (learn more)
Humana Press, Molecular Medicine, New York, New World, Boehringer Mannheim, Difco Laboratories, Cold Spring Harbor, Papua New Guinea, Academic Press, Nucleic Acids Res, Amersham Pharmacia, Parasite Immunol, Growth Inhibition Assays, Laboratory Manual, Becton Dickinson, Fisher Scientific, Libraries Using Subtractive Hybridization, Roche Molecular Biochemicals, Elodie Belnoue, Erythrocytic-Stage Mouse Models, Intracellular Cytokine Staining, New England Biolabs, Palo Alto, Roche Diagnostics, Southern Blotting of Parasite
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