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Mammalian and Avian Transgenesis: New Approaches (Principles and Practice)
 
 
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Mammalian and Avian Transgenesis: New Approaches (Principles and Practice) [Hardcover]

Shirley Pease (Editor), Carlos Lois (Editor)

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Book Description

Principles and Practice December 21, 2005
Mammalian and Avian Transgenesis presents a collection of novel methods for the production of a wide range of transgenic animals. The manual focuses largely on mice, but also contains protocols for successful transgenesis in rats, cows, pigs and birds. The manual provides detailed, step-by-step protocols covering all aspects of the production of transgenic animals, including the use of lentiviral vectors in gene transfer, intracytoplasmic sperm injection, nuclear transfer, large insert transgenesis, conditional gene expression systems, the use of reporter genes in transgenesis and transgenesis in large animals and birds. The text is supplemented by superb color photos. While the focus is on newly established techniques, the fundamental methods of transgenesis are also covered for those new to the field. Thus this manual is perfectly suited for those wishing to adopt new technologies in transgenesis.

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Inside This Book (learn more)
Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
cumulus mass cells, embryo complementation, enucleation pipette, floxed line, avian transgenesis, doubly mutant mice, embryo transfer pipette, enucleation medium, microinjection pipette, tetraploid blastocysts, grid dish, transgenic birds, micromanipulation chamber, ovarian fat pad, fertilization drops, viral solution, fertilization medium, tetraploid embryos, mosaic founders, holding pipette, embryo culture medium, dox treatment, activation medium, hyaluronidase solution, maturation medium
Key Phrases - Capitalized Phrases (CAPs): (learn more)
Carlos Lois, Shirley Pease, Biol Reprod, Proc Natl Acad Sci, Cold Spring Harbor, Kevin Eggan, Rudolf Jaenisch, Nat Biotechnol, Specialty Media, Springer-Verlag Berlin Heidelberg, Ancillary Techniques, Nat Genet, Dev Biol, Kent Lloyd, Cloning the Laboratory Mouse, Massachusetts Institute of Technology, Materials Required, Mol Reprod Dev, Nucleic Acids Res, Reprod Fertil, Swiss Webster, Nat Heintz, Shiaoching Gong, Sprague Dawley, Beckman Avanti
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