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Mass Spectrometry for Biotechnology
 
 

Mass Spectrometry for Biotechnology [Paperback]

Gary Siuzdak (Author)
4.5 out of 5 stars  See all reviews (10 customer reviews)

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Book Description

0126474710 978-0126474718 February 1, 1996 1
If you investigate biological systems and might use mass spectrometry in your research but need to know more about it, this book is for you. It introduces the fundamental concepts of mass spectrometry and how mass spectrometers work. It also presents recent advancements particularly interesting to bio-researchers in an easy-to-understand manner that does not require extensive background in chemistry, math, or physics.

Key Features
* Glossary of basic terms
* Abundant illustrations
* Examples of applications
* Practical tips on using mass spectrometric techniques
* Useful for peptide, protein, oligonucleotide, and carbohydrate analysis
* Simplified description of mass spectrometry including:
* Matrix-Assisted Laser Desorption/Ionization (MALDI)
* Electrospray Ionization (ESI)
* Fast Atom/Ion Bombardment (FAB)

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Editorial Reviews

Review

"...an excellent...source of information on the fundamental aspects of mass spectrometry for the nonexpert, who wishes to use mass spectrometry."
--AMERICAN SOCIETY FOR MASS SPECTROMETRY

From the Back Cover

Only recently has it been possible to use mass spectrometry for proteins and other large biomolecules most interesting to cell and molecular biologists, biochemists, and biotechnologists. Most bioresearchers do not have sufficient training in mass spectrometry to maximize its usefulness to their research. This book attempts to bring these researchers up to speed.
Mass Spectrometry for Biotechnology introduces the fundamental concepts of mass spectrometry and explains how mass spectrometers work. It also presents recent advances of particular interest to bio-researchers in an easy-to-understand manner than does not require extensive background in chemistry, math, or physics.
Key Features
* Glossary of basic terms
* Abundant illustrations
* Examples of applications
* Practical tips on using mass spectrometric techniques
* Useful for peptide, protein, oligonucleotide, and carbohydrate analysis
* Simplified description of mass spectrometry including:
* Matrix-Assisted Laser Desorption/Ionization (MALDI)
* Electrospray Ionization (ESI)
* Fast Atom/Ion Bombardment (FAB)

Product Details

  • Paperback: 161 pages
  • Publisher: Academic Press; 1 edition (February 1, 1996)
  • Language: English
  • ISBN-10: 0126474710
  • ISBN-13: 978-0126474718
  • Product Dimensions: 8.9 x 5.9 x 0.5 inches
  • Shipping Weight: 12 ounces (View shipping rates and policies)
  • Average Customer Review: 4.5 out of 5 stars  See all reviews (10 customer reviews)
  • Amazon Best Sellers Rank: #1,012,192 in Books (See Top 100 in Books)

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Customer Reviews

10 Reviews
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Average Customer Review
4.5 out of 5 stars (10 customer reviews)
 
 
 
 
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Most Helpful Customer Reviews

8 of 8 people found the following review helpful:
5.0 out of 5 stars Biological Mass Spectrometry in a Nutshell, May 10, 2000
By A Customer
This review is from: Mass Spectrometry for Biotechnology (Paperback)
The book is clear and concise, written in a manner that is not too sophisticated for the novice and not too simplistic for the seasoned spectrometrist. The book eloquently conveys the art of mass spectrometry, from the fundamentals of instrumentation to the diversity of applications. It is ideal for teaching situations, whether introductory level instrumental analysis courses (few textbooks can rival the quality and quantity of instrumental content) or higher-level mass spectrometry courses (spectral interpretation and applications galore.) Most certainly, this should find a home on the bookshelf of every mass spectrometry lab! If you are wondering whether mass spectrometry would be helpful for your own research and aren't sure what questions to ask, look no further--a great comparison of instrumental features and their adaptability to biological situations.
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7 of 7 people found the following review helpful:
5.0 out of 5 stars A quick way to get started with mass spectrometry, December 1, 1999
By A Customer
This review is from: Mass Spectrometry for Biotechnology (Paperback)
Most other mass spectrometry books are about general uses that do not include proteins and other large biomolecules. This book is a good way to get up to speed on the primary technologies and techniques used to identify proteins using mass spectrometry. It is clearly written and strikes a good balance between breadth and depth of coverage. The tables give good summaries of alternatives and comparisons between them. I found the scientific literature much more accessible after reading this book.

Many sections are followed by references, which is a good start for continuing on. Annotation and a consistent format would make them a more useful resource.

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4 of 4 people found the following review helpful:
5.0 out of 5 stars Very useful, direct and presents recent advances, great book, June 3, 1999
By A Customer
This review is from: Mass Spectrometry for Biotechnology (Paperback)
This is a very easy to follow book that introduces and explains how mass spectrometers work. It also presents recent advnaces of particular interest to scientists of all levels who think of MS only useful with small molecules. I found it especially usefull in MS analysis of proteins with MALDI-TOF.
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Inside This Book (learn more)
First Sentence:
This chapter describes sample introduction and ionization. Read the first page
Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
alkali cationization, tandem mass analysis, protein ladder, declustering potential, limited mass range, monoisotopic mass, electrospray mass spectrum, electron ejection, given empirical formula, valley definition, multiple charging, mass analyzer, charge state distribution, matrix peaks, reference ions, ionization techniques, electrostatic analyzer, collision cell, electron ionization, direct insertion probe, fragment ions, picomole level, mass accuracy, matrix ions, kinetic energy distribution
Key Phrases - Capitalized Phrases (CAPs): (learn more)
New York, Rapid Commun, Positive Deprotonation, The Scripps Research Institute, Advantages Disadvantages Practical
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