A collection of key techniques for the study of receptors and transport proteins. The book provides examples of how different membrane proteins can be overexpressed in both prokaryotic and eukaryotic expression systems, how natural and overexpressed proteins can be solubilized from their host membranes, and how the solubilized protein can be purified in active form. Each protocols contains step-by-step instructions to ensure success, troubleshooting advice, lists of reagents, and tips on avoiding pitfalls.
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Book Description
Editorial Reviews
Review
"Investigators working with membrane proteins will want to acquire this book. This is a useful compilation of protocols. . .there is much of utility in this book"-Doody's Health Sciences Book Review Journal
"...a useful reference work for researchers investigating the structure and function of membrane proteins." -- Immunology News
"...provides good food for thought for new and advanced membrane protein researchers." - ChemBioChem
"...highly recommendable to people working in proteomics..." - Proteomics Journal
"...a useful reference work for researchers investigating the structure and function of membrane proteins." -- Immunology News
"...provides good food for thought for new and advanced membrane protein researchers." - ChemBioChem
"...highly recommendable to people working in proteomics..." - Proteomics Journal
From the Back Cover
Recent developments in molecular biology and biochemistry have made it possible to determine the three-dimensional structure of membrane proteins and so come to a full understanding of their function. In Membrane Protein Protocols: Expression, Purification, and Characterization, researchers at major universities and research centers around the world describe in detail the key techniques that have proven successful in the study of receptors and transport proteins. The book provides examples of how different membrane proteins can be overexpressed in both prokaryotic and eukaryotic expression systems, how natural and overexpressed proteins can be solubilized from their host membranes, and how the solubilized protein can be purified in active form. Each protocol contains step-by-step instructions to ensure success, troubleshooting advice, lists of reagents, and tips on avoiding pitfalls.
Timely and highly practical, Membrane Protein Protocols: Expression, Purification, and Characterization offers investigators proven methods to determine the three-dimensional structure of membrane receptors and transport proteins, as well as proven practical help in the discovery of new clinical targets for the design of novel pharmaceutical agents useful in treating cancer, bacterial and viral infection, and many metabolic diseases.
Timely and highly practical, Membrane Protein Protocols: Expression, Purification, and Characterization offers investigators proven methods to determine the three-dimensional structure of membrane receptors and transport proteins, as well as proven practical help in the discovery of new clinical targets for the design of novel pharmaceutical agents useful in treating cancer, bacterial and viral infection, and many metabolic diseases.
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Inside This Book
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First Sentence:
Cytochrome b5 (cyt b5) is an electron transfer protein that exists in a membrane-bound form in the endoplasmic reticulum where it is anchored to the membrane via a carboxyl-terminal transmembrane -helix (1-3). Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
preequilibration buffer, flippase activity, receptor pseudotypes, metrizamide interface, rat liver carnitine palmitoyltransferase, ultrafiltration assembly, uptake buffer, pastoris cells, sucrose monolaurate, raft fraction, solubilization protocol, glycerol facilitator, solubilized membrane fraction, standard enzyme assay, membrane protein expression, total membrane fraction, assay cocktail, solubilization buffer, zinc transporter, reconstitution buffer, biosensor surface, lipidic cubic phases, rat liver membranes, serine palmitoyltransferase, dihydroorotate dehydrogenase Key Phrases - Capitalized Phrases (CAPs): (learn more)
Characterization Edited, Humana Press Inc, Membrane Protein Protocols, New York, Cold Spring Harbor, Purification of Zinc Transporter, Amersham Biosciences, Protein A-agarose, Boehringer Mannheim, Heterologous Membrane Protein Expression, Purification of Erythrocyte Membrane Transporters, Solubilization of Integral Membrane Protein, Life Technologies, Methods Enzymol, Purification of Porins, Amersham Pharmacia Biotech, Lipidic Phases, New England, Amiloride-Sensitive Epithelial Sodium Channel, Cell Biol, Millipore Corp, Purification of Pancreastatin Receptor, Rabbit Cytochrome, Reconstitution of Flippase Activity, The Protein Protocols Handbook New!
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Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Cytochrome b5 (cyt b5) is an electron transfer protein that exists in a membrane-bound form in the endoplasmic reticulum where it is anchored to the membrane via a carboxyl-terminal transmembrane -helix (1-3). Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
preequilibration buffer, flippase activity, receptor pseudotypes, metrizamide interface, rat liver carnitine palmitoyltransferase, ultrafiltration assembly, uptake buffer, pastoris cells, sucrose monolaurate, raft fraction, solubilization protocol, glycerol facilitator, solubilized membrane fraction, standard enzyme assay, membrane protein expression, total membrane fraction, assay cocktail, solubilization buffer, zinc transporter, reconstitution buffer, biosensor surface, lipidic cubic phases, rat liver membranes, serine palmitoyltransferase, dihydroorotate dehydrogenase Key Phrases - Capitalized Phrases (CAPs): (learn more)
Characterization Edited, Humana Press Inc, Membrane Protein Protocols, New York, Cold Spring Harbor, Purification of Zinc Transporter, Amersham Biosciences, Protein A-agarose, Boehringer Mannheim, Heterologous Membrane Protein Expression, Purification of Erythrocyte Membrane Transporters, Solubilization of Integral Membrane Protein, Life Technologies, Methods Enzymol, Purification of Porins, Amersham Pharmacia Biotech, Lipidic Phases, New England, Amiloride-Sensitive Epithelial Sodium Channel, Cell Biol, Millipore Corp, Purification of Pancreastatin Receptor, Rabbit Cytochrome, Reconstitution of Flippase Activity, The Protein Protocols Handbook New!
Books on Related Topics | Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Citations (learn more)
This book cites 32
books:
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- Protein Protocols on DVD-ROM (CD-ROM Digital Video Disk, Version 2.0) by John M. Walker in Front Matter, and page 161
- Liposome Methods and Protocols (Methods in Molecular Biology) by Subhash C. Basu in Front Matter
- Neurogenetics: Methods and Protocols (Methods in Molecular Biology) by Nicholas T. Potter in Front Matter
- Molecular Cloning: A Laboratory Manual, Third Edition (3 volume set) by Joe Sambrook on page 101
- Molecular Mechanisms in Visual Transduction, Volume 3 (Handbook of Biological Physics) by D.G. Stavenga on page 86
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