Membrane Transporters: Methods and Protocols (Methods in Molecular Biology) [Hardcover]

Qing Yan (Editor)

Book Description

Publication Date: May 2, 2003 | ISBN-10: 1588291049 | ISBN-13: 978-1588291042 | Edition: 1

This collection of cutting-edge methodologies for studying membrane transporters and channels takes advantage of all the latest developments in biomedical research, including pharmacogenomcs, bioinformatics, and microarray technologies. The authors explain databases and tools for bioinformatics studies, provide practical guidelines for microarray experiments and data analysis, and illustrate the use of small angle X-ray scattering, nuclear magnetic resonance (NMR), and molecular modeling to study the structural biology of membrane transporters. Methods for exploring structure-function correlation, such as site-directed mutagenesis, immunocytochemistry, and confocal microcopy are also described, along with several that may help in the development of novel therapeutics.

Editorial Reviews

Review

"The book is a useful reference to have for bench scientists. The protocols provide a good starting point for someone seeking technical advice for various procedures used to study transport."-Doody's Health Sciences Book Review Journal "...a valuable laboratory or office protocols book that combines, distils and updates traditional and novel bench and in silico techniques for the study of membrane transporters." - Clinical and Experimental Pharmacology and Physiology

From the Back Cover

Membrane transporters and channels play key roles in drug absorption and distribution, influence drug efficacy and toxicity, and are the target of about 30% of currently marketed drugs. In Membrane Transporters: Methods and Protocols, a panel of recognized basic and clinical investigators-many world-famous-describe their proven, cutting-edge methodologies for studying these important proteins. Treated in step-by-step detail, these techniques take advantage of all the latest developments in biomedical research, including pharmacogenomics, bioinformatics, and microarray technologies. The authors explain databases and tools for bioinformatics analysis, provide practical guidelines for microarray experiments and data analysis, and illustrate the use of small-angle X-ray scattering, nuclear magnetic resonance (NMR), and molecular modeling to study the structural biology of membrane transporters. Methods for exploring structure-function correlation, such as site-directed mutagenesis, immunocytochemistry, and confocal microscopy, are also described, along with several methods that may help in the development of novel therapeutics. Each technique contains hands-on instructions, many notes on avoiding the problems that one may encounter, and suggestions for variant procedures. Comprehensive and highly practical, Membrane Transporters: Methods and Protocols offers investigators working in biopharmaceutical science, drug discovery, and drug delivery both an array of powerful tools for work on membrane transporters and an essential guide to the state-of-the-art in the membrane transporter field today.

Product Details

• Hardcover: 384 pages
• Publisher: Humana Press; 1 edition (May 2, 2003)
• Language: English
• ISBN-10: 1588291049
• ISBN-13: 978-1588291042
• Product Dimensions: 9.2 x 6.1 x 1.3 inches
• Shipping Weight: 2.2 pounds (View shipping rates and policies)
• Average Customer Review: 5.0 out of 5 stars  See all reviews (1 customer review)
• Amazon Best Sellers Rank: #3,362,581 in Books (See Top 100 in Books)

Customer Reviews

Most Helpful Customer Reviews

1 of 2 people found the following review helpful:

5.0 out of 5 stars A comprehensive book, March 19, 2006

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Personalized Medicine - See all my reviews

This review is from: Membrane Transporters: Methods and Protocols (Methods in Molecular Biology) (Hardcover)

A comprehensive and practical book on doing research in membrane transporters. Those protocols are up to date and helpful.