Methods in Cellular Imaging [Hardcover]

Ammasi Periasamy (Editor)

Book Description

Publication Date: December 15, 2001 | ISBN-10: 0195139364 | ISBN-13: 978-0195139365 | Edition: 1

Advances in technology have revolutionized the development of light microscopy techniques in biomedical research, thus improving visualization of the microstructure of cells and tissues under physiological conditions. Fluorescence microscopy methods are non-contact and non-invasive and provide high spatial and temporal resolution that other laboratory techniques cannot. This well-illustrated book targets graduate students and scientists who are new to the state-of-the-art fluorescence microscopy techniques used in biological and clinical imaging. It explains basic concepts and imaging procedures for wide-field, confocal, multiphoton excitation, fluorescence resonance energy transfer (FRET), lifetime imaging (FLIM), spectral imaging, fluorescence recovery after photobleaching (FRAP), optical tweezers, total internal reflection, high spatial resolution atomic force microscopy (AFM), and bioluminescence imaging for gene expression. The usage of these techniques in various biological applications, including calcium, pH, membrane potential, mitochondrial signaling, protein-protein interactions under various physiological conditions, and deep tissue imaging, is clearly presented. The authors describe the approaches to selecting epifluorescence microscopy, the detectors, and the image acquisition and processing software for different biological applications. Step-by-step directions on preparing different digital formats for light microscopy images on websites are also provided.

Editorial Reviews

Review

"This is a timely and useful book that includes the basics while focusing on the newest and most promising advances in fluorescence microscopy and imaging: 2-photon, confocal, FRET, and FLIM technologies for biological and clinical applications. It is highly practical, with important technical details and sources of information, but it also develops the pertinent theory and principles. It's a must-buy for anyone engaged in fluorescence microscopy!"--Professor Alan F. Horwitz, Department of Cell Biology, University of Virginia, Charlottesville, Virginia

"A thoughtfully selected blend of basic principles and methodological details renders this book an excellent source of information. Concise and well-written chapters lead the reader right to the point, which is of great use when considering applying a particular method."--Stefan W. Hell, Priv.-Doz., Dr. rer. nat. Head, High Resolution Optical Microscopy Group, Max-Planck-Institute for Biophysical Chemistry, Gottingen, Germany

"Advanced imaging techniques are in the midst of a critical transition from something the average scientist only reads about to something that is becoming a part of his or her research. This book offers a set of excellent chapters that introduce the reader to the techniques in enough detail to have an impact on their research in a real way. I recommend it to those interested in beginning to apply these exciting new techniques."--Prof. Scott Fraser, Biological Imaging Center, Beckman Institute, California Institute of Technology, Pasedena, California

"Methods in Cellular Imaging presents the hardware, methods, and applications of the new microsopy, and also covers the optics and physics that are important to the field. The result is a compendium that is of great value for both beginners and experienced microscopists. It can serve as a guide for the novice or the perplexed, a manual for the practitioner, and a reference handbook for both."--Perspective: Book Review, March 2002

"On the whole, the work is a tour-de-force of cutting-edge microscopy techniques.--Microscopy and Analysis

About the Author

Ammasi Periasamy, Professor Biology and Biomedical Engineering, University of Virginia.

Product Details

• Hardcover: 448 pages
• Publisher: Oxford University Press, USA; 1 edition (December 15, 2001)
• Language: English
• ISBN-10: 0195139364
• ISBN-13: 978-0195139365
• Product Dimensions: 10.3 x 7.4 x 1.2 inches
• Shipping Weight: 3.6 pounds
• Amazon Best Sellers Rank: #3,640,008 in Books (See Top 100 in Books)