Modern HPLC for Practicing Scientists [Paperback]

Michael W. Dong (Author)

Book Description

ISBN-10: 047172789X | ISBN-13: 978-0471727897 | Publication Date: June 12, 2006 | Edition: 1st

A comprehesive yet concise guide to Modern HPLC

Written for practitioners by a practitioner, Modern HPLC for Practicing Scientists is a concise text which presents the most important High-Performance Liquid Chromatography (HPLC) fundamentals, applications, and developments. It describes basic theory and terminology for the novice, and reviews relevant concepts, best practices, and modern trends for the experienced practitioner. Moreover, the book serves well as an updated reference guide for busy laboratory analysts and researchers.

Topics covered include:

• HPLC operation
• Method development
• Maintenance and troubleshooting
• Modern trends in HPLC such as quick-turnaround and "greener" methods
• Regulatory aspects

While broad in scope, this book focuses particularly on reversed-phase HPLC, the most common separation mode, and on applications for the pharmaceutical industry, the largest user segment. Accessible to both novice and intermedate HPLC users, information is delivered in a straightforward manner illustrated with an abundance of diagrams, chromatograms, tables, and case studies, and supported with selected key references and Web resources.

With intuitive explanations and clear figures, Modern HPLC for Practicing Scientists is an essential resource for practitioners of all levels who need to understand and utilize this versatile analytical technology.

Editorial Reviews

Review

"This reasonably priced HPLC primer provides sound and authoritative guidance…" (E-STREAMS, June 2007)

Review

"[...] a concise and 'to-the-point' text, covering the broad topic of HPLC. While the book is not intended to be a comprehensive treatise, it addresses all major topics in HPLC and provides updated, practical information not found in other introductory texts. I found the author's use of bullet points, tabulation and figures extremely effective in conveying practical 'take-home' messages and providing sound and definitive guidance." --Henrik T. Rasmussen, PhD

"This is a great introductory book on liquid chromatography. I especially like the intuitive explanations and clear figures." --John W. Dolan, PhD

"Dong's book is especially written for the pharmaceutical industry. I am sure that it will be highly welcomed by the practitioners in this field, not only because of its numerous relevant examples of drug separations but also because of its focus on regulatory aspects." --Veronika Meyer, PhD

See all Editorial Reviews

Product Details

• Paperback: 304 pages
• Publisher: Wiley-Interscience; 1st edition (June 12, 2006)
• Language: English
• ISBN-10: 047172789X
• ISBN-13: 978-0471727897
• Product Dimensions: 6.2 x 0.6 x 9.4 inches
• Shipping Weight: 14.4 ounces (View shipping rates and policies)
• Average Customer Review: 4.8 out of 5 stars  See all reviews (9 customer reviews)
• Amazon Best Sellers Rank: #132,542 in Books (See Top 100 in Books)
  • #36 in Books > Professional & Technical > Professional Science > Chemistry > Analytic

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Customer Reviews

Most Helpful Customer Reviews

4 of 4 people found the following review helpful:

5.0 out of 5 stars MOdern HPLC for Practicing Scientists, June 3, 2007

By 

F. Lin (Short Hills, NJ) - See all my reviews
(REAL NAME)   

This review is from: Modern HPLC for Practicing Scientists (Paperback)

HPLC has become one of the most widely applied chemical analytical techniques in the pharmaceutical industry. Dr Dong's book, Modern HPLC for Practicing Scientists, provides excellent source of information and knowledge for both beginners and highly experienced professionals who utilize this analytical tool. Chapter 1 presents an overview of chromatographic principles while the 2nd chapter deals with fundamental aspects of HPLC and considerations that enhance efficient separation. In chapter 3, Dr. Dong discusses the chromatographic column both from theoretical perspectives and applications. This is very valuable to practicing scientists.

In Chapter 4, the book provides a concise overview of HPLC instrumentation, operating principles as well as recent advances in instrumentation such as Waters Acquity UPLCTM (Chapter 11). A lot of information is provided on practical issues associated with the use of HPLC systems and the pictures and illustrations make their understanding much easier.

Chapter 5 provides a hands-on approach to HPLC analysis including mobile phase preparation and parameter optimization. This is a great chapter for a novel analyst who is often saddled with numerous options on the choice of HPLC parameters. Dr. Dong's attention to details makes it possible even for the novice chromatographer to undertake a full HPLC procedure with little or no supervision

Although Chapters 6 and 7 deal with applications in the pharmaceutical, food and environmental industries, the practicing scientist who this text is best suited for, would benefit more by integrating information from these chapters with the fundamental and practical concepts documented in the earlier chapters.

The inclusion of method development and regulatory issues with respect to HPLC techniques and applications in chapters 8 and 9 is particularly commendable. Industrial R & D scientists who are not only challenged to develop and optimize methods but also work within the constraints of regulatory agencies would find these chapters immensely rewarding. These chapters provide hands-on guidance for laboratory scientists in the pharmaceutical and other regulatory industries.

Chapter 10, dealing with troubleshooting is one of the special attributes of this book. This chapter provides quick fix approach to resolving some of the HPLC's often encountered problems. This chapter is bound to save some cost because more often than not, the HPLC analyst tend to vainly envisage and explore more catastrophic problems ignoring the simple remedies that are succinctly documented in Chapter 10.

Overall, Dr. Dong's book is very well written. Each aspect of the text is clearly and succinctly presented, making it easy to understand. The chapter on maintenance and troubleshooting guide (Chapter 11) should be a great asset. Dr. Dong has managed, in a very basic but meticulous manner, to produce an essential text for both the novice and experienced HPLC practitioner in the pharmaceutical industry.

2 of 2 people found the following review helpful:

3.0 out of 5 stars Good for intro, but if you have any expereince you'll need to go elsewhere, October 11, 2010

By 

Cas40 - See all my reviews

Amazon Verified Purchase

This review is from: Modern HPLC for Practicing Scientists (Paperback)

I'll start by saying I think the book is okay for what it is, and that is a primer, more like 'HPLC lite'. To its credit, it does have a brief overview of most any topic, but this amounts to an ocean of knowledge that is one inch deep. I think users will quickly have to move on to more elaborate texts like High Performance Gradient Elution by Dolan and Snyder, or Trace Quantitative Analysis by Mass Spectrometry by Boyd, Basic and Bethem. For instance, there is only one chapter on ADME and pharmaceutical analysis, and that topic alone could easily fill a book. I appreciate that the author is trying to make a general overview, but the topic is so broad and LC is becoming so specialized that it seems like a quixotic quest to try to condense all of that knowledge down into an introductory text. Might be good for new hires in the lab, but I don't see much use for more experienced scientists.
On the plus side, the Best Practices sections in the operation guide is a great resource for someone new in the field who needs to know the 'rules' of HPLC.

2 of 2 people found the following review helpful:

5.0 out of 5 stars A User's Guide for All Levels, October 24, 2007

By 

Lorraine M. Henriques (Portland, OR) - See all my reviews
(REAL NAME)   

This review is from: Modern HPLC for Practicing Scientists (Paperback)

In this concise and very affordable book, Dr. Dong has compiled the most important concepts for anyone interested in the fundamentals of HPLC. He has also taken great care to provide clear illustrations to bring across these concepts, which, like many images, speak a thousand words. In a world inundated with almost too much information, it is very refreshing to have a text in hand that one can read through, understand, and put to use in a short amount of time. As a former research scientist turned Technical Sales Rep, I appreciate that I can put this book to work for me. Every chromatographer, new or experienced, needs to have this book on their shelf, or better yet, on top of the lab bench.