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Molecular Biology: A Project Approach [Paperback]

Susan J. Karcher (Author)
4.0 out of 5 stars  See all reviews (1 customer review)

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Book Description

November 29, 1995 0123977207 978-0123977205
This course manual instructs students in recombinant DNA techniques and other essential molecular biology techniques in the context of projects. The project approach inspires and captivates students; it involves them in the scientific experience, providing continuity to laboratory bench time and an understanding of the principles underlying the techniques presented.
Molecular Biology is a must for any department, operating under budgetary constraints that offers or plans to offer a course in molecular cloning.

Key Features
* Includes a glossary of over 200 terms important for understanding molecular biology
* Uses an inexpensive source of eukaryotic cells - great for schools on a budget
* Includes Methods Locator that provides instant access to the latest methods
* Contain clearly written, easy-to-follow, student-tested instructions:
* Sterile techniques
* Phage titration
* Gel electrophoresis of DNA
* Restriction enzyme digestion
* Plasmid isolation
* Transformation of E. Coli
* Recombinant DNA cloning
* Nick translation labeling
* Nonradioactive primer labelling
* Nonradioactive DNA detection
* Southern blotting
* Colony hybridization
* Purification of plant DNA
* RNA purification
* Northern blotting
* Purification of poly A+ RNA
* Polymerase chain reaction (PCR)

Editorial Reviews

Review

"This book is excellent in its technical and practical details. The background information, clear instructions and copious supporting information about recipes, sources, and further readings should make it possible for any student to successfully complete the experiments and gain experience with these techniques."
--Sara Abraham in CANADIAN JOURNAL OF MEDICAL LABORATORY SCIENCE
"Once again an extensive introduction to the techniques in this chapter make this book worth having just because of the background material provided."
--Cynthia M. Galloway in PLANT SCIENCE BULLETIN
"...very valuable. The book, which is a guideline for instructors, may also serve as a rapid source of protocols for scientists not used to working in molecular biology. Its reasonable price should contribute to the foreseeable success of this book."
--JOURNAL OF PLANT PHYSIOLOGY

From the Back Cover

This manual bridges the gap between theory and protocol. By presenting extensive background information as well as easy-to-understand and easy-to-follow protocols, this book allows students to perform and understand the latest techniques in molecular biology. Thus, it can serve as the sole textbook for molecular biology methods courses.
Molecular Biology: A Project Approach gives students an understanding of the significance of the techniques they are learning and their theoretical basis within the framework of integrated projects. This project approach provides continuity and simulates a real research experience.
Includes a glossary of over 200 terms important for understanding molecular biology
Key Features
* Includes a glossary of over 200 terms important for understanding molecular biology
* Uses an inexpensive source of eukaryotic cells - great for schools on a budget
* Contain clearly written, easy-to-follow, student-tested instructions
* Includes Methods Locator that provides instant access to the latest methods

Product Details

  • Paperback: 280 pages
  • Publisher: Academic Press (November 29, 1995)
  • Language: English
  • ISBN-10: 0123977207
  • ISBN-13: 978-0123977205
  • Product Dimensions: 9.3 x 7.8 x 0.8 inches
  • Shipping Weight: 1.5 pounds (View shipping rates and policies)
  • Average Customer Review: 4.0 out of 5 stars  See all reviews (1 customer review)
  • Amazon Best Sellers Rank: #3,893,803 in Books (See Top 100 in Books)

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4.0 out of 5 stars Very appropriate text for beginning molecular biologists., April 9, 1999
By A Customer
This review is from: Molecular Biology: A Project Approach (Paperback)
This text was used in an undergraduate molecular biology lab course which i took, and it contained detailed information and suggestions which were extremely useful for beginning students. This text has continued to be useful, however, as a reference for commonly used protocols and for troubleshooting experiments.
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Inside This Book (learn more)
First Sentence:
A transposon is a genetic entity that promotes its own movement or transposition; it can insert as a discrete segment of DNA into many different sites in the genome. Read the first page
Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
chemiluminogenic substrate, photobiotin labeling, oligo labeling, pool plates, phage buffer, unique restriction endonuclease sites, target duplication, tetracycline resistance determinant, gel loading dye, inoculation loop, phage lysate, prehybridization solution, top agar, nonradioactive labeling, nick translation reaction, transposon mutagenesis, star activity, different restriction endonucleases, bent glass rod, sterile test tubes, microfuge tube, gel box, supernatant solution, formaldehyde gel, generalized transduction
Key Phrases - Capitalized Phrases (CAPs): (learn more)
Nucleic Acids Res, Academic Press, San Diego, Cold Spring Harbor, Trends Genet, New York, Suggested Reading, Laboratory Manual, Trends Biochem, Cell Biol, Oxford University Press, Bacterial Genetics, Component Final, Plant Mal, Plant Phys, Purdue University, San Francisco, Van Brunt
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