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Molecular Biology Techniques: An Intensive Laboratory Course
 
 

Molecular Biology Techniques: An Intensive Laboratory Course [Paperback]

Walt Ream (Author), Katharine G. Field (Author)
5.0 out of 5 stars  See all reviews (1 customer review)

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Book Description

November 26, 1998 0125839901 978-0125839907
This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression.

Key Features
* Provides well-tested experimental protocols for each technique
* Lists the reagents and preparation of each experiment separately
* Contains a complete schedule of experiments and the preparation required
* Includes study questions at the end of each chapter

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Molecular Biology Techniques: An Intensive Laboratory Course + At the Bench: A Laboratory Navigator, Updated Edition + Lab Math: A Handbook of Measurements, Calculations, and Other Quantitative Skills for Use at the Bench
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Editorial Reviews

From the Back Cover

This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression.
Key Features
* Provides well-tested experimental protocols for each technique
* Lists the reagents and preparation of each experiment separately
* Contains a complete schedule of experiments and the preparation required
* Includes study questions at the end of each chapter

About the Author

Walt Ream, Professor of Microbiology, Oregon State University, received the Richard M. Bressler Senior Faculty Teaching Award for developing the course this book supports. In addition to this work, he has written numerous peer reviewed scholarly papers, review articles, book articles and chapters and has co-authored another book entitled, Molecular Biology Techniques: An Intensive Laboratory Course. His research led to a discovery for which a patent was issued in 2004. He regularly reviews grant proposals for NSF, USDA, DOE, NIH, American Cancer Society, NSERC-Canada, Lindbergh Foundation, Corporation for Plant Biotechnology Research and he reviews papers for Journal of Bacteriology, Plant Molecular Biology, PNAS, Science, Molecular & General Genetics, Microbiological Reviews, Molecular Plant-Microbe Interactions, Biochemistry, Journal of Biological Chemistry, Plasmid, Gene, Plant Physiology, Journal of American Horticulture Society, and Molecular Microbiology. He is a member of Genetics Society of America, American Society for Microbiology, and American Association for the Advancement of Science.


Product Details

  • Paperback: 234 pages
  • Publisher: Academic Press (November 26, 1998)
  • Language: English
  • ISBN-10: 0125839901
  • ISBN-13: 978-0125839907
  • Product Dimensions: 9.1 x 6.7 x 0.7 inches
  • Shipping Weight: 1 pounds (View shipping rates and policies)
  • Average Customer Review: 5.0 out of 5 stars  See all reviews (1 customer review)
  • Amazon Best Sellers Rank: #1,491,024 in Books (See Top 100 in Books)

 

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11 of 15 people found the following review helpful:
5.0 out of 5 stars complete basic tool, April 27, 2000
This review is from: Molecular Biology Techniques: An Intensive Laboratory Course (Paperback)
Anyone who need very nice information should read it.The book provide quite day to day information to fit lab routine.It Also explain some impresive insight on future technics. Wiht out doubt I recomended.I gave it 5 star because I use it everyday at work. Lab.Gen e Molecular Biology. InCor. Brazil
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Inside This Book (learn more)
First Sentence:
The purpose of CsCl-ethidium bromide density gradient centrifugation is to separate supercoiled (covalently closed circular) plasmid DNA from other components present in lysates of bacterial cells: linear DNA (chromosomal fragments), nicked plasmid DNA (which lacks supercoils), RNA, proteins, and carbohydrates. Read the first page
Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
load buffer, mutagenic oligonucleotide, nick translation kit, resuspended cells, acrylamide stock, ligase buffer, phagemid vector, bromphenol blue, blot procedure, resolving gel, tac promoter, restriction digest, dissolve pellet, microfuge tube
Key Phrases - Capitalized Phrases (CAPs): (learn more)
Protocol Beforehand, Safety Precautions Wear
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