Yao-Shan Fan and a panel of senior scientists and pioneering researchers describe in step-by-step style the leading FISH techniques and those molecular technologies beyond FISH available for diagnostic services in genetics and oncology. The methods include labeling FISH probes for DNA and RNA targets, fluorescence genotyping, CGH microarray, spectral karyotyping/multicolor FISH, and primed in situ labeling. There are also techniques for multicolor fiber FISH, multi-telomere FISH, prenatal diagnosis using maternal blood, and preimplantation diagnosis. Oncological methods include simultaneous fluorescence immunophenotyping and FISH for leukemia and lymphoma, HER2 amplification in breast cancer, and CAC/PAC for cancer cytogenetics.
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Editorial Reviews
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"Dr. Fan has done a marvelous job of putting together a collection of laboratory protocols and review articles of different FISH technologies...Molecular Cytogenetics: Protocols and Applications is packed with information on cutting-edge FISH technologies and their applications, limitations, and pitfalls, along with detailed laboratory protocols. I would certainly recommend that this book be included in the libraries of cytogeneticists and cytogenetic laboratories" - Am. J. Hum. Gent. "Dr. Fan has done a marvelous job of putting together a collection of laboratory protocols and review articles of different FISH technologies. The authors of the different chapters not only gave detailed, easy-to-follow protocols, but also provided their first-hand experiences of troubleshooting. Molecular Cytogenetics: Protocols and Applications is packed with information on cutting-edge FISH technologies and their applications, limitations, and pitfalls, along with detailed laboratory protocols. I would certainly recommend that this book be included in the libraries of cytogeneticists and cytogenetic laboratories." -The American Society of Human Genetics
From the Back Cover
With the proliferation of fluorescence in situ hybridization (FISH) techniques as a powerful diagnostic tool in many areas of medicine, the variety and complexity of tests pose bewildering choices even for long-experienced laboratory technologists and physicians. In Molecular Cytogenetics: Protocols and Applications, Yao-Shan Fan and a panel of senior scientists and pioneering researchers describe in step-by-step fashion not only the leading FISH techniques, but also those molecular technologies beyond FISH now available for diagnostic services in genetics and oncology. The methods include labeling FISH probes for DNA and RNA targets, fluorescence genotyping, CGH microarray, spectral karyotyping/multicolor FISH, and primed in situ labeling. There are also techniques for multicolor fiber FISH, multi-telomere FISH, prenatal diagnosis using maternal blood, and preimplantation diagnosis. Oncological methods include simultaneous fluorescence immunophenotyping and FISH for leukemia and lymphoma, HER2 amplification in breast cancer, and CAC/PAC for cancer cytogenetics. Each easy-to-follow protocol offers first hand advice on how to obtain the best results, to avoid pitfalls, and to troubleshoot problems. Many protocols discuss possible future applications of the technology and explain its sensitivity and limitations. Cutting-edge and highly practical, Molecular Cytogenetics: Protocols and Applications offers laboratory physicians and scientists today's most comprehensive and up-to-date collection of FISH protocols for cancer diagnosis and investigation, plus an array of the emerging molecular technologies beyond FISH.
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First Sentence:
The word "chromosome" was introduced over a century ago from the Greek language meaning "colored body." Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
screening cryptic telomeric rearrangements, target metaphase slides, abnormal reference range, nicking buffer, ethanol jar, variety available can, telomere clones, reviewed probes, rearrangements involving telomeres, hybridized area, fetal nucleated red blood cells, soak coverslip, yellow fusion signal, conventional cytogenetic studies, excess wash solution, antibody suspension, chromosome paint probes, aneuploidy detection, supernumerary marker chromosomes, chromosome microdissection, subtelomeric rearrangements, trisomic cell line, uncultured amniocytes, two green signals, subtelomeric probes Key Phrases - Capitalized Phrases (CAPs): (learn more)
Life Technologies, New York, Humana Press Inc, Cancer Genet, Downers Grove, Cell Genet, Vector Laboratories, Cancer Res, Molecular Probes, Acids Res, Laboratory Supplies, Roche Diagnostics, American College of Medical Genetics, Cytocell Ltd, Applied Spectral Imaging, Genes Chromosom, Promega Corp, Fisher Healthcare, Slide Pretreatment, Cell Biol, Applied Biosystems, Fisher Scientific, Grand Island, Multicolor Fiber, System Kit New!
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Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
The word "chromosome" was introduced over a century ago from the Greek language meaning "colored body." Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
screening cryptic telomeric rearrangements, target metaphase slides, abnormal reference range, nicking buffer, ethanol jar, variety available can, telomere clones, reviewed probes, rearrangements involving telomeres, hybridized area, fetal nucleated red blood cells, soak coverslip, yellow fusion signal, conventional cytogenetic studies, excess wash solution, antibody suspension, chromosome paint probes, aneuploidy detection, supernumerary marker chromosomes, chromosome microdissection, subtelomeric rearrangements, trisomic cell line, uncultured amniocytes, two green signals, subtelomeric probes Key Phrases - Capitalized Phrases (CAPs): (learn more)
Life Technologies, New York, Humana Press Inc, Cancer Genet, Downers Grove, Cell Genet, Vector Laboratories, Cancer Res, Molecular Probes, Acids Res, Laboratory Supplies, Roche Diagnostics, American College of Medical Genetics, Cytocell Ltd, Applied Spectral Imaging, Genes Chromosom, Promega Corp, Fisher Healthcare, Slide Pretreatment, Cell Biol, Applied Biosystems, Fisher Scientific, Grand Island, Multicolor Fiber, System Kit New!
Books on Related Topics | Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Citations (learn more)
This book cites 45
books:
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25
books
cite this book:
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- Cancer Cytogenetics by Sverre Heim on page 102, and page 389
- Preimplantation Genetics by A. Kuliev on page 271, and page 272
- Prenatal Diagnosis by Mark Evans on page 219, and page 235
- Health Sciences by James Anderson in Front Matter (1), and Front Matter (2)
- Preimplantation Genetic Diagnosis by Joyce C. Harper on page 271, and page 272
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- Cancer Cytogenetics: Methods and Protocols (Methods in Molecular Biology) by John Swansbury in Front Matter (1), and Front Matter (2)
- PCR Detection of Microbial Pathogens (Methods in Molecular Biology) by Konrad Sachse in Front Matter
- In Situ Detection of DNA Damage: Methods and Protocols (Methods in Molecular Biology, Vol 203) (Methods in Molecular Biology) by Vladimir V. Didenko in Front Matter
- Inflammation Protocols (Methods in Molecular Biology) by Paul G. Winyard in Front Matter
- Lentivirus Gene Engineering Protocols (Methods in Molecular Biology) by Maurizio Federico in Front Matter
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