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Molecular Diagnosis of Infectious Diseases (Methods in Molecular Medicine)
 
 
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Molecular Diagnosis of Infectious Diseases (Methods in Molecular Medicine) [Hardcover]

Jochen Decker (Editor), Udo Reischl (Editor)

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Book Description

1588292215 978-1588292216 December 1, 2003 2
This second edition of a classic laboratory manual describes cutting-edge methods for the protein-based diagnosis of infectious diseases. Explaining the latest developments in genomics, proteomics, bioinformatics, biosensors, high-throughput devices, and recombinant technology, the authors apply these new methodologies successfully to the identification and characterization of valuable diagnostic markers, immunomodulatory components, epitope mapping, the production and purification of recombinant antigens, as well as to diagnostic reagents in immunological assays.

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Editorial Reviews

Review

"The comprehensive nature of the book makes it an invaluable tool for industry and academia for managing the large amount of data generated in bioinformatics. Each chapter is written in the usual scientific paper format of an abstract, introduction, materials, methods, notes or conclusions and references. The index is well done and makes the book particularly helpful as a reference manual. "-Doody's Health Sciences Book Review Journal "...Anyone who has the interest and resources to develop novel assays not only for infectious diseases but also for other diseases will benefit tremendously from this book." -Archives of Pathology and Laboratory Medicine "...a valuable methodological book for molecular biologists in a hospital laboratory or in academia." -Trends in Parasitology "...a powerful tool for developing better experiments in the diagnostics of infectious diseases." -International Microbiology "...a very good compilation of techniques that can be used for numerous applications." -Analytical Biochemistry "...a useful reference source for anyone who wants to manipulate microbial protein for diagnostic purposes." -International Journal of Medical Microbiology "...this book continues the well-established format of this series from Humana Press in providing clearly described laboratory protocols with useful background discussion...recommended as a useful laboratory reference book..." - Proteomics "The comprehensive nature of the book makes it an invaluable tool for industry and academia for managing the large amount of data generated in bioinformatics." -Doody's Health Sciences and Book Review Journal "...well-organized...provides detailed methods and practical tips...the book can be recommended for students, graduate students, and postdoctoral, fellows in the field of proteomics..." - Clinical Infectious Diseases "...brings together the key elements of oral medicine and nutrition in an accessible form for the dental professional...an excellent reference text...wholeheartedly recommend(ed)." - American Society for Clinical Nutrition Review of the first edition: "This work represents one of the most up-to-date molecular biology protocols books to date. . .Anyone who has the interest and resources to develop novel assays not only for infectious diseases but also for other diseases will benefit tremendously from this book. . a valuable purchase to keep as a quick protocol source." -Archives of Pathology and Laboratory Medicine

From the Back Cover

In this second edition of a much-acclaimed classic laboratory manual, Molecular Diagnosis of Infectious Diseases, leading experts from academia and industry describe cutting-edge methods for the protein-based diagnosis of infectious diseases. Explaining the latest developments in genomics, proteomics, bioinformatics, biosensors, high-throughput devices, and recombinant technology, the authors demonstrate the power of these new methodologies in both established and novel procedures by applying them successfully to the identification and characterization of valuable diagnostic markers, immunomodulatory components, epitope mapping, the production and purification of recombinant antigens, and their use in diagnostic reagents in immunological assays. Basic problems of serological laboratory diagnostics and the use of immunoproteomics for discovering target proteins are also addressed. Each readily reproducible protocol is described in step-by-step detail and includes a background introduction, equipment and reagent lists, tips on troubleshooting and avoiding known pitfalls, and extensive references. Comprehensive and up-to-date, Molecular Diagnosis of Infectious Diseases, Second Edition offers novice investigators an easy entry into the field and experienced researchers a broad array of time saving tools that will not only elucidate new principles, but also greatly facilitate the development and successful application of rapid diagnostic methods.

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Inside This Book (learn more)
First Sentence:
Proteomics has been widely applied to develop two-dimensional polyacrylamide gel electrophoresis maps and databases, evaluate gene expression profiles under different environmental conditions, assess global changes associated with specific mutations, and define drug targets of bacterial pathogens. Read the first page
Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
splenocyte medium, measles virus nucleocapsid protein, pulse renaturation, cell strainer, nondenaturing agarose gel, inclusion body preparation, breaking buffer, clinical prevalence, blotted proteins, diagnostic antigens, diffusion dependence, peptide stocks, thiol coupling, biomolecular interaction analysis, peptide pools, splenic cells, receptor layer, folding conditions, sonication buffer, reaction plate, adsorbed antigen, capture capacity, dissociation phases, lac repressor protein, recombinant antigens
Key Phrases - Capitalized Phrases (CAPs): (learn more)
Molecular Diagnosis of Infectious Diseases, Molecular Medicine, Humana Press Inc, New York, Magnetic Agarose Beads, Whole Gel Eluter, Cold Spring Harbor, Becton Dickinson Labwares, New England Biolabs, Cell Biol, Methods Enzymol, Nucleic Acids Res, Roche Molecular Biochemicals, The Protein Protocols Handbook, Amersham Pharmacia Biotech, Boca Raton, Charles River, Laboratory Manual, Methods Enzvmol, Protein Expr, Roche Diagnostics, San Diego, Applied Biosystems, Beckman Coulter, Determination of Epitopes
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