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Molecular Diagnostic PCR Handbook
 
 
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Molecular Diagnostic PCR Handbook [Hardcover]

Gerrit J. Viljoen (Editor), Louis H. Nel (Editor), John R. Crowther (Editor)

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Book Description

1402034032 978-1402034039 July 19, 2005 1
This book gives a comprehensive account of the practical aspects of PCR and strong consideration is given to ensure its optimal use in a laboratory environment. This includes the theoretical aspects related to PCR, setting-up of a PCR laboratory and good Laboratory Practice and standardised PCR protocols to detect animal disease pathogens. Examples of Standard Operating Procedures as used in individual specialist laboratories and an outline of training materials necessary for PCR technology transfer are presented. The difficulties, advantages and disadvantages in PCR applications are explained and placed in context with other test systems. Emphasis is placed on the use of PCR for detection of pathogens with a particular focus on diagnosticians and scientists from the developing world. It is hoped that this book will enable readers from various disciplines and levels of expertise to better judge the merits of PCR and to increase their skills and knowledge in order to assist in a more logical, efficient and assured use of this technology.

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Inside This Book (learn more)
Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
titre positive sample, photo documentation system, assay file, times during sample preparation, abortus vaccine strain, containing client information, repeat pipettor, hazardous samples, mail the following day, clean microcentrifuge tube, top microcentrifuge, initial homogenization, rapid detection method, malignant catarrhal fever, carryover contamination, diagnostic samples, allantoic fluid, hybridisation probes, reception board, loading buffer, laminar flow cabinets, agarose gel stained, primer concentration
Key Phrases - Capitalized Phrases (CAPs): (learn more)
Nucl Acids Res, Presence of Brucella, South Africa, Courtesy of Roche Diagnostics, Equipment Micropipettes, Preconditions All, Promega Taq, Reception Office, Takara Ex Taq, Type Primer, Anal Biochem, Courtesy of Onderstepoort Veterinary Institute, Dnase Faulty, Observation Inhibitors, Perkin Elmer, Proc Natl Acad Sci, Safety Hazardous, Springer-Verlag Berlin Heidelberg
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