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Molecular Embryology: Methods and Protocols (Methods in Molecular Biology)
 
 
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Molecular Embryology: Methods and Protocols (Methods in Molecular Biology) [Hardcover]

Paul T. Sharpe (Editor), Ivor Mason (Editor)

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Molecular Embryology: Methods and Protocols (Methods in Molecular Biology) Molecular Embryology: Methods and Protocols (Methods in Molecular Biology)
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Book Description

January 15, 1999 0896033872 978-0896033870 1st
A comprehensive guide to cutting-edge methods used today across the dramatically growing field of molecular embryology. These powerful techniques take advantage of the most commonly used vertebrate experimental models: murine embryos for their genetics, chick embryos for in vivo manipulation, zebrafish for mutagenesis, amphibian embryos, and nonvertebrate chordates. The book emphasizes the most important methods of experimental molecular biology and the particular advantages of each different species. Detailed, easy-to-follow instructions, together with relevant background information and helpful tips, ensure easy reproducibility.


Editorial Reviews

Review

"This book has much to offer biologists interested in the molecular biology of developmental systems in a wide range of vertebrates."-Doody's Health Sciences Book Review Journal

"Universal techniques are well-written, complete, and easy to follow including in situ hybridization, differential display PCR, as well as standard protocols for library construction, Northern and Southern analyses. This would be an excellent addition to any developmental biology laboratory, university department, and especially good for graduate students and post-docs."-Teratology

"...this book is a tome, a necessity imposed on it by its breadth, both in terms of model organisms (mouse, chicken, amphibians, zebra fish and prochordates) and methods (surgery, retro-virology, in situ hybridization, molecular biology, immunohistochemistry, microscopy and photography.) The protocols are compact and clearly written, with ever-so-helpful hints (called notes) grouped at the end of each chapter. ...we encourage both teachers and researchers actively involved in experimental embryology to buy this book, put it on the shelf and let your students know it is there." - TINS

"Molecular Embryology provides an excellent source for understanding the strengths and weaknesses of the various vertebrate model organisms, and provides a ready source for highly useful protocols....The protocols are thoroughly described and are accompanied by helpful illustrations. Developmental biologists will be pleased to add this book to their collection, and will likely reference it often for its many useful protocols."- The Quarterly Review of Biology

"This book is of interest to embryologists, biologists and probably to some neonatologists and geneticists. It describes in detail the experimental use of animal embryos...A well illustrated and referenced text for specialists." - Journal of Endocrine Genetics

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Inside This Book (learn more)
First Sentence:
The laboratory mouse, Mus musculus, is the developmental biologist's mammal of choice for studies of development. Read the first page
Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
conditionally immortal cell lines, astrocyte cell lines, prospective neural tissue, lipophilic membrane dyes, albumen medium, transplantation apparatus, blastemal cells, transgenic frog embryos, secretory trap vector, coarse forceps, diploid screen, gastrula wall, vertical signaling, collagen bed, mesoderm induction assays, recombined tissues, amputation plane, right wing bud, transgenesis procedure, mouse primordial germ cells, polyester wax, gene interference, microinjection pipet, apical ridge, sandwich explant
Key Phrases - Capitalized Phrases (CAPs): (learn more)
Boehringer Mannheim, Humana Press Inc, Molecular Embryology, Cold Spring Harbor, Howard's Ringer, Nucleic Acids Res, Cell Biol, New York, Genes Dev, Microinjection of Cloned, San Diego, Trends Genet, Practical Approach, Life Technologies, Laboratory Manual, Materials All, Nonmammalian Embryos, Micromanipulation of Embryos, Boeringer Mannheim, Cell Res, Oxford University Press, Mouse Stem Cells, Wellcome Trust, Dow Corning, Home Office
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