Detailed protocols for analyzing DNA strand breaks, specific RNA/DNA expressions, and DNA binding proteins such as transcription factors at the individual cell level. It describes in situ nick translation and TUNEL in detail, along with radioactive and non-radioactive methods for in situ hybridization. Of special significance is the description of Southwestern histochemistry, which makes it possible to analyze the expression of transcription regulatory factors in individual cells. This volume provides an overview of the current status of molecular histochemistry along with practical how-to-do-it details. The methodology is set out in easy-to-follow language by leading scientists working in the field today. Both a rich source of the latest information and a practical lab manual.
Molecular Histochemical Techniques (Springer Lab Manuals)
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Molecular Histochemical Techniques presents detailed protocols for analyzing DNA strand breaks, specific RNA/DNA expressions, and DNA binding proteins such as transcription factors at the individual cell level. In situ nick translation and TUNEL are described in detail, along with radioactive and nonradioactive methods for in situ hybridization. Of special significance is the description of Southwestern histochemistry, a new development in molecular histochemistry, which makes it possible to analyze the expression of transcription regulatory factors in individual cells. This volume provides an overview of the current status of molecular histochemistry along with practical how-to-do-it details. Methodology is set forth step-by-step in easy-to-follow language by leading scientists who are working in the field today. For researchers, laboratory technicians, and graduate students, the book serves as both a rich source of the latest information and a practical lab manual.
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First Sentence:
DNA strand breaks, especially single-stranded breaks (SSB), are very commonly found through the life cycle of cells. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
moist chamber humidified, southwestern histochemistry, preembedding electron microscopic, quartz dish, postembedding electron microscopic, dimer method, preembedding method, postembedding method, biotinylated tyramine, metal intensification, combined immunohistochemistry, transcription regulatory factors, brief washing, situ nick translation, thermal controller, hybridization mix, denaturation solution, chromogen solution, nonradioactive probe, immunogold particles, antifade solution, fresh frozen sections, prehybridization buffer, mixed bed resin, rat pituitary glands Key Phrases - Capitalized Phrases (CAPs): (learn more)
Histochem Cytochem, Boehringer Mannheim Biochemicals, Cell Biol, Boehringer Industries, Proc Natl Acad Sci, Acta Pathol Jpn, Nagasaki University School of Medicine, References Adams, Electron Microsc, Cancer Res, Department of Pathology, Detection of Transcription Regulatory Proteins, Rinse Brij, Toshiba Ltd, Vector Laboratories, Biochem Biophys Res Commun, Dev Biol, Exp Cell Res, Faculty of Engineering, Faculty of Medicine, Kyowa Medex, Light Microscopical, Nucl Acids Res, Second Department of Surgery, The University of Tokushima New!
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Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
DNA strand breaks, especially single-stranded breaks (SSB), are very commonly found through the life cycle of cells. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
moist chamber humidified, southwestern histochemistry, preembedding electron microscopic, quartz dish, postembedding electron microscopic, dimer method, preembedding method, postembedding method, biotinylated tyramine, metal intensification, combined immunohistochemistry, transcription regulatory factors, brief washing, situ nick translation, thermal controller, hybridization mix, denaturation solution, chromogen solution, nonradioactive probe, immunogold particles, antifade solution, fresh frozen sections, prehybridization buffer, mixed bed resin, rat pituitary glands Key Phrases - Capitalized Phrases (CAPs): (learn more)
Histochem Cytochem, Boehringer Mannheim Biochemicals, Cell Biol, Boehringer Industries, Proc Natl Acad Sci, Acta Pathol Jpn, Nagasaki University School of Medicine, References Adams, Electron Microsc, Cancer Res, Department of Pathology, Detection of Transcription Regulatory Proteins, Rinse Brij, Toshiba Ltd, Vector Laboratories, Biochem Biophys Res Commun, Dev Biol, Exp Cell Res, Faculty of Engineering, Faculty of Medicine, Kyowa Medex, Light Microscopical, Nucl Acids Res, Second Department of Surgery, The University of Tokushima New!
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Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
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