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Molecular Methods for Virus Detection
 
 
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Molecular Methods for Virus Detection [Paperback]

Danny L. Wiedbrauk (Editor), Daniel H. Farkas (Editor)

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Book Description

January 17, 1995 0127489207 978-0127489209 1
Molecular diagnostic procedures have been described in a number of recent books and articles. However, these publications have not focused on virus detection, nor have they provided practical protocols for the newer molecular methods.
Written by the inventors or principal developers of these technologies, Molecular Methods for Virus Detection provides both reviews of individual methods and instructions for detecting virus nucleic acid sequences in clinical specimens. Each procedure includes quality assurance protocols that are often ignored by other methodology books. Molecular Methods for Virus Detection provides clinically relevant procedures for many of the newer diagnostic methodologies.

Key Features
* Provides state-of-the-art PCR methods for amplification, quantitation, in situ hybridization, and multiplex reactions
* Goes beyond PCR with protocols for 3SR, NASBA, LCR, SDA, and LAT
* Covers important virus detection methods such as in situ hybridization; Southern, dot, and slot blots; branched chain signal amplification; and chemiluminescence
* Includes quality control information crucial in research and clinical laboratories
* Most chapters are written by the inventors and principal developers of the methodologies
* Includes color plates, 77 figures, and 18 tables

Editorial Reviews

Review

"A particular strength of many of the chapters is the emphasis which has been placed on identifying problems that can arise when introducing and performing the tests and discussion of relative advantages and disadvantages... The clearly presented protocols should enable many research and development departments to develop in house assays."
--D. Cubitt, in THE JOURNAL OF CLINICAL PATHOLOGY

From the Back Cover

Molecular diagnostic procedures have been described in a number of recent books and articles. However, these publications have not focused on virus detection, nor have they provided practical protocols for the newer molecular methods.
Written by the inventors or principal developers of these technologies, Molecular Methods for Virus Detection provides both reviews of individual methods and instructions for detecting viral and mycobacterial nucleic acid sequences in clinical specimens. Each procedure includes quality assurance protocols that are often ignored by other methodology books. Molecular Methods for Virus Detection provides clinically relevant procedures for many of the newer diagnostic methodologies.
Key Features
* Provides state-of-the-art PCR methods for amplification, quantitation, in situ hybridization, and multiplex reactions
* Goes beyond PCR with protocols for 3SR, NASBA, LCR, SDA, and LAT
* Covers important virus detection methods such as in situ hybridization; Southern, dot, and slot blots; branched chain signal amplification; and chemiluminescence
* Includes quality control information crucial in research and clinical laboratories
* Most chapters are written by the inventors and principal developers of the methodologies
* Includes color plates, 77 figures, and 18 tables

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Inside This Book (learn more)
First Sentence:
Viral infections are the most common cause of human disease, and are responsible for at least 60% of the illnesses that prompt patients to visit a physician (Ray, 1979). Read the first page
Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
hemagglutination buffer, molecular virology laboratory, chemiluminometric immunoassay, control wicks, antiviral susceptibility testing, wicking agent, amplifying solution, tuberculosis target, cycling probe technology, nonisotopic detection methods, target complementary sequence, clinical virology laboratory, molecular diagnostic procedures, molecular diagnostic methods, carryover contamination, dot blot hybridization assay, drug dilutions, primer dimerization, molecular diagnostic laboratory, dioxetane substrate, susceptibility assay, digestion reagent, sensitivity panel, human serum samples, target nucleic acid
Key Phrases - Capitalized Phrases (CAPs): (learn more)
Academic Press, Nucleic Acids Res, New York, San Diego, Digene Diagnostics, Cold Spring Harbor, Jeanne Carr, Agents Chemother, Roy Sooknanan, United States, Francois Ferre, Chiron Corporation, American Society of Clinical Pathologists, Marcel Dekker, Probe Techniques, Royal Oak, Bio Techniques, Conclusion References, Conclusions References, Diagnostic Molecular Microbiology, Laboratory Manual, Antiviral Res, Applied Biosystems, Boehringer Mannheim, Corning Glass Works
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