This volume attempts to describe the purification and assay of the diverse group of proteins involved in otoplasmic organization. Some of the more promising attempts to apply techniques of molecular biology, genetics, and molecular cytochemistry to these proteins are discussed.
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"The Methods in Enzymology series represents the gold-standard."
--NEUROSCIENCE
"Incomparably useful."
--ANALYTICAL BIOCHEMISTRY
"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page."
--BIO/TECHNOLOGY
"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection."
--CHEMISTRY IN INDUSTRY
"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced."
--AMERICAN SOCIETY OF MICROBIOLOGY NEWS
"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work."
--ENZYMOLOGIA
"A series that has established itself as a definitive reference for biochemists."
--JOURNAL OF CHROMATOGRAPHY
"The Methods in Enzymology series represents the gold-standard."
--NEUROSCIENCE
"Incomparably useful."
--ANALYTICAL BIOCHEMISTRY
"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page."
--BIO/TECHNOLOGY
"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection."
--CHEMISTRY IN INDUSTRY
"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced."
--AMERICAN SOCIETY OF MICROBIOLOGY NEWS
"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work."
--ENZYMOLOGIA
"A series that has established itself as a definitive reference for biochemists."
--JOURNAL OF CHROMATOGRAPHY
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Inside This Book
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First Sentence:
The 110-kDa protein-calmodulin complex present in the microvilli of chicken intestinal brush borders is the first protein from a higher eukaryote that has been shown by both biochemical and genetic studies to exhibit many properties characteristic of the myosin I class of mechanoenzymes. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
microtubule affinity columns, biotinylated vimentin, hydroxylapatite buffer, erythrocyte tubulin, dynein extract, unbound actin, disassembly buffer, myosin head fragments, tubulin samples, lens vimentin, brush border extract, reactivation solution, photolabeled peptides, assembly buffer containing, brain microtubule protein, trypanosome cytoskeleton, inner arm mutants, tyrosination state, heavy chain kinase, microtubule pellet, brain cytoplasmic dynein, vanadate concentration, cycled microtubules, outer arm dynein, kinase elutes Key Phrases - Capitalized Phrases (CAPs): (learn more)
Cell Biol, Academic Press, Cell Motil, New York, Palo Alto, Cell Sci, Cold Spring Harbor, Nucleic Acids Res, Green A-Sepharose, San Diego, Cell Mold, Cell Res, General Considerations, Biotechnology Inc, Cell Movement, Coomassie Brilliant Blue, Muscle Res, Procion Red-agarose, Supernatant Pellet, Bio-Rad Laboratories, Gamete Res, National Cancer Institute, Malachite Green, New England Nuclear, Ace Glass New!
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Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
The 110-kDa protein-calmodulin complex present in the microvilli of chicken intestinal brush borders is the first protein from a higher eukaryote that has been shown by both biochemical and genetic studies to exhibit many properties characteristic of the myosin I class of mechanoenzymes. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
microtubule affinity columns, biotinylated vimentin, hydroxylapatite buffer, erythrocyte tubulin, dynein extract, unbound actin, disassembly buffer, myosin head fragments, tubulin samples, lens vimentin, brush border extract, reactivation solution, photolabeled peptides, assembly buffer containing, brain microtubule protein, trypanosome cytoskeleton, inner arm mutants, tyrosination state, heavy chain kinase, microtubule pellet, brain cytoplasmic dynein, vanadate concentration, cycled microtubules, outer arm dynein, kinase elutes Key Phrases - Capitalized Phrases (CAPs): (learn more)
Cell Biol, Academic Press, Cell Motil, New York, Palo Alto, Cell Sci, Cold Spring Harbor, Nucleic Acids Res, Green A-Sepharose, San Diego, Cell Mold, Cell Res, General Considerations, Biotechnology Inc, Cell Movement, Coomassie Brilliant Blue, Muscle Res, Procion Red-agarose, Supernatant Pellet, Bio-Rad Laboratories, Gamete Res, National Cancer Institute, Malachite Green, New England Nuclear, Ace Glass New!
Books on Related Topics | Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Citations (learn more)
This book cites 25
books:
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- Cell Movement, The Dynein ATPases (Volume 1) by Fred D. Warner on 5 pages
- Detoxication and Drug Metabolism: Conjugation and Related Systems, Volume 77: Volume 77: Detoxification and Drug Metabolism (Methods in Enzymology) by Nathan P. Kaplan in Front Matter (1), and Front Matter (2)
- Guide to Yeast Genetics and Molecular Biology, Volume 194: Volume 194: Guide to Yeast Genetics and Molecular Biology (Methods in Enzymology) by John N. Abelson in Front Matter
- Chemical Modification of Proteins by Gary E. Means on page 450
- Perspectives in Andrology (Serono Symposia Publications from Raven Press) (Vol 53) by Mario Serio on page 419
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