Natural Killer Cell Protocols: Cellular and Molecular Methods (Methods in Molecular Biology) [Hardcover]

Kerry S. Campbell (Editor), Marco Colonna (Editor)

Book Description

Publication Date: February 4, 2000 | ISBN-10: 0896036839 | ISBN-13: 978-0896036833 | Edition: 1

Kerry S. Campbell and Marco Colonna have assembled a comprehensive collection of readily reproducible methods designed to study natural killer (NK) cells from the broadest variety of viewpoints. These include not only classic techniques, but also new approaches to standard methods, newly evolved techniques that have become valuable for specific applications, and unique models for manipulating and studying NK cells. Among the advanced methods covered are those for in vitro transendothelial migration, in vivo detection of cells migrating into tumors, immunofluorescence staining of intracellular cytokines, and in vitro NK cell development. Valuable techniques for specific applications include vaccinia virus protein expression, soluble KIR-Fc fusions for HLA class I binding assays, calcium mobilization in cell conjugates, and identification of heterodimeric receptor complexes using cDNA library expression cloning. Natural Killer Cell Protocols: Cellular and Molecular Methods offers immunologists, cancer researchers, virologists, and cell biologists today's most comprehensive collection of both established and cutting-edge techniques, methods that will contribute significantly to advancing our understanding of this fascinating and critically important class of cells.

Editorial Reviews

Review

"Natural Killer Cell Protocols is a worthwhile addition to the reference collection of an academic library that serves scientists who are conducting research on NK cells, lymphocytes, cancer, and/or viral infections. It is well representative of the previous volumes in the Methods in Molecular Biology series and therefore is an important acquisition for libraries collecting the series in its entirety."-E-Streams (Electronic Reviews of Science and Technology References "Besides clearly describing each step in the assays, the very detailed protocols point out potential trouble spots and provide those small points that make a technique workable. This is a fine "cookbook" to use to get started on NK cell assays."-Doody's Health Science Book Review Journal "The book contains 31 chapters contributed by experts in the field....The chapters are generally well written and informative. Experimental procedures are presented in great detail, which should be very helpful for those who are unfamiliar with the methodologies involved. An especially useful feature is the inclusion of notes at the end of each chapter in which potential problems and pitfalls are describe and methods for dealing with them are suggested. The appendices provide a handy summary of monoclonal antibodies and cell lines that are commonly used in NK cell studies....this book is an excellent compendium of laboratory methods relevant to research on NK cells. It should prove valuable not only to novices embarking upon NK cell work, but also to experienced investigators who wish to explore a new facet of these intriguing lymphocytes." - Hematologic Oncology

From the Back Cover

As the research has continued, it has become increasingly clear that natural killer (NK) cells are critical sentinels of the innate immune response, playing important roles in protecting the body from numerous pathogens and cancer in addition to contributing to normal pregnancy and impacting the outcomes of transplantation. While the first edition provided a valuable collection of classical cellular and in vivo techniques to study NK cell functions, the Second Edition of Natural Killer Cell Protocols: Cellular and Molecular Methods brings together more recently developed methods, more refined techniques, and detailed protocols designed to study NK cells within specialized tissue sites in both mice and humans. In this collection of methods, international leaders in the field cover topics ranging from the analysis of the various stages of NK cell development and maturation to specialized techniques for the identification of ligands for NK cell receptors. This volume also includes an appendix, providing a rich resource summarizing available reagents to study NK cells, cross-referencing KIR nomenclature, and detailing the many HLA ligands for various KIR family members. As a volume in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and thorough notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Natural Killer Cell Protocols: Cellular and Molecular Methods, Second Edition seeks to aid researchers and further advance our understanding of the functions, maturation, and regulation of these fascinating and dynamic cells. --This text refers to an alternate Hardcover edition.

Product Details

• Hardcover: 408 pages
• Publisher: Humana Press; 1 edition (February 4, 2000)
• Language: English
• ISBN-10: 0896036839
• ISBN-13: 978-0896036833
• Product Dimensions: 9.3 x 6.4 x 1.3 inches
• Shipping Weight: 1.7 pounds (View shipping rates and policies)
• Amazon Best Sellers Rank: #3,249,813 in Books (See Top 100 in Books)