In Neural Stem Cells: Methods and Protocols, internationally recognized experts from academic, clinical, and pharmaceutical laboratories describe in great detail the most frequently used cellular, molecular, and electrophysiological methods to isolate, characterize, and utilize neural stem cells. These readily reproducible techniques introduce the various sources of stem/progenitor cells, provide a wide range of conditions for their culture, and make it possible to define their properties in culture. Additional techniques are designed to help researchers identify endogenous stem cells as well as exogenous stem cells after transplantation in the brain. The protocols range from the simplest methods of isolation and characterization of neural cell properties to such very sophisticated methods as characterizing gene expression, telomerase assays, and cell cycle kinetics.
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"...the book will be of value to stem cell researchers, as the protocols are presented in sufficient detail to make the replication of results likely between laboratories. Neural Stem Cells is a competently collected and presented set of methods." -Cell Therapy News
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Differentiation of pluripotent embryonic stem (ES) cells into specific lineages is an important source of cells for implantation and gene delivery, as well as a useful model to study patterns of differentiation and gene expression during the very early development of the mammalian embryo (1). Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
human neural stem cell lines, retroviral vector supernatant, crest stem cells, spinal cord stem cells, stem cell progeny, stem cell grafting, engrafted cells, brain cannula, nongenetic methods, neural stem cells, tissue culture equipment, panning dish, ensheathing cells, inducible clones, olfactory ensheathing glia, adult rat spinal cord, compound microscope equipped, cloning medium, newborn cells, neural progenitor cells, luciferase assay reagent, normal horse serum, multipotential stem cells, glass micropipet, packaging cell line Key Phrases - Capitalized Phrases (CAPs): (learn more)
Humana Press Inc, Brain Res, Boehringer Mannheim, Molecular Probes, Electron Microscopy Sciences, Cold Spring Harbor, Fisher Scientific, Route of Cellular Administration, Brain Disease Models, Cell Biol, Engineered Stem Cells, New York, Cell Transplant, Vector Labs, Becton Dickinson, Eastman Kodak, Fetal Tissue, Genehunter Corp, Isolation of Novel Genes, Preparation of Neural Progenitors, Telomerase Assays, Tet System Approved Fetal Bovine Serum, Texas Red, Analysis of Neuronal Differentiation, Evan Snyder New!
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Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Differentiation of pluripotent embryonic stem (ES) cells into specific lineages is an important source of cells for implantation and gene delivery, as well as a useful model to study patterns of differentiation and gene expression during the very early development of the mammalian embryo (1). Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
human neural stem cell lines, retroviral vector supernatant, crest stem cells, spinal cord stem cells, stem cell progeny, stem cell grafting, engrafted cells, brain cannula, nongenetic methods, neural stem cells, tissue culture equipment, panning dish, ensheathing cells, inducible clones, olfactory ensheathing glia, adult rat spinal cord, compound microscope equipped, cloning medium, newborn cells, neural progenitor cells, luciferase assay reagent, normal horse serum, multipotential stem cells, glass micropipet, packaging cell line Key Phrases - Capitalized Phrases (CAPs): (learn more)
Humana Press Inc, Brain Res, Boehringer Mannheim, Molecular Probes, Electron Microscopy Sciences, Cold Spring Harbor, Fisher Scientific, Route of Cellular Administration, Brain Disease Models, Cell Biol, Engineered Stem Cells, New York, Cell Transplant, Vector Labs, Becton Dickinson, Eastman Kodak, Fetal Tissue, Genehunter Corp, Isolation of Novel Genes, Preparation of Neural Progenitors, Telomerase Assays, Tet System Approved Fetal Bovine Serum, Texas Red, Analysis of Neuronal Differentiation, Evan Snyder New!
Books on Related Topics | Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Citations (learn more)
This book cites 38
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40
books
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- Short Protocols in Molecular Biology (2 volume set) by Frederick M. Ausubel on page 212, and page 221
- Molecular Cloning: A Laboratory Manual, Third Edition (3 volume set) by Joe Sambrook on page 212, and page 221
- Nucleic Acids (Methods in Molecular Biology) by John M. Walker in Front Matter
- Protocols for Neural Cell Culture by Sergey Fedoroff on page 155
- Liposome Methods and Protocols (Methods in Molecular Biology) by Subhash C. Basu in Front Matter
See all 38 books this book cites
- Calcium-Binding Protein Protocols: Volume 1: Reviews and Case Histories (Methods in Molecular Biology) by Hans J. Vogel in Front Matter
- Cell Migration in Inflammation and Immunity: Methods and Protocols (Methods in Molecular Biology) by Daniele D'Ambrosio in Front Matter
- PCR Cloning Protocols (Methods in Molecular Biology) by Bing-Yuan Chen in Front Matter
- High Throughput Screening: Methods and Protocols (Methods in Molecular Biology) (World's Best Reading) by William P. Janzen in Front Matter
- Tumor Suppressor Genes: Volume 1: Pathways and Isolation Strategies (Methods in Molecular Biology) by Wafik S. El-Deiry in Front Matter
See all 40 books citing this book
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