Oxidative Stress and Antioxidant Protocols (Methods in Molecular Biology) [Hardcover]

Donald Armstrong (Editor)

Book Description

Publication Date: February 15, 2002 | Series: Methods in Molecular Biology (Book 186)

Donald Armstrong updates and expands his highly praised Free Radical and Antioxidant Protocols (Humana, 1998) with a collection of new and valuable methods for evaluating the pertubations in cell function resulting from increased oxidative stress. Presented in a user-friendly, step-by-step format, these readily reproducible techniques cover both free radical-derived and antioxidant biomarkers. The methodologies demonstrated include ELISA, HPLC, infrared spectroscopy, gas chromatography-mass spectroscopy, immunoblotting, electroelution fractionation, isoelectric focusing, voltametry, and electron paramagnetic resonance imaging. Special emphasis is given to the separation of complex mixtures of plant antioxidants, soft drug design to protect from toxic oxidative metabolites, in vitro oxidation conditions, and correcting for random measurement error to improve statistical interpretation. A soon-to-be-published companion volume, Oxidants and Antioxidants: Ultrastructural and Molecular Biology Protocols (0-89603-851-3), contains state-of-the-art molecular and ultrastructural methods that expand the total number of protocols to 109 assays.

Editorial Reviews

Review

"The book could be used effectively by experienced or novice basic science researchers or clinicians since the protocols are written clearly and concisely. Each of the chapters in the book gives specific instructions on how to prepare specimens for analysis. The chapters are clearly written, concise, and should be easy for anyone to follow. . .This book is intended and written as a guide to use in the laboratory. It would be quite useful for anyone wishing to assess many parameters of oxidative activities in biological specimens."-doody's Health Sciences Book Review Journal "This is a superb book, a second volume edited by Donald Armstrong on this topic...There are 33 chapters in the 322 pages of this text therefore, each is commendably succinct and to the point. There are, unfortunately, far too many chapters to list all the topics covered, but it is extensive and amazing." - Free Radical Biology & Medicine

From the Back Cover

Oxidative stress has been linked to a variety of medical problems, including cardiovascular disease, cancer, arthritis, diabetes, and aging. Drawing on the growing volume of research into the impact of oxidative stress on pathophysiological mechanisms, Donald Armstrong updates and expands his highly praised Free Radical and Antioxidant Protocols (Humana, 1998) with a collection of new and valuable methods for evaluating the perturbations in cell function resulting from increased oxidative stress. Presented in a user-friendly, step-by-step format by accomplished investigators from prestigious universities, institutes, and laboratories around the world, these readily reproducible techniques cover both free radical-derived and antioxidant biomarkers. The methodologies demonstrated include ELISA, HPLC, infrared spectroscopy, gas chromatography-mass spectroscopy, immunoblotting, electroelution fractionation, isoelectric focusing, voltammetry, and electron paramagnetic resonance imaging. Special emphasis is given to the separation of complex mixtures of plant antioxidants, soft drug design to protect from toxic oxidative metabolites, in vitro oxidation conditions, and correcting for random measurement error to improve statistical interpretation. A soon-to-be-published companion volume, Oxidants and Antioxidants: Ultrastructure and Molecular Biology Protocols (Humana, 2002), contains state-of-the-art molecular and ultrastructural methods that expand the total number of protocols to 109 assays. Cutting-edge and highly practical, Oxidative Stress Biomarkers and Antioxidant Protocols provides many powerful new tools for detecting oxidative stress and for investigating the possibilities for therapeutic intervention monitoring in a variety of serious diseases.

Product Details

• Hardcover: 336 pages
• Publisher: Springer; 1st edition (February 15, 2002)
• Language: English
• ISBN-10: 0896038505
• ISBN-13: 978-0896038509
• Product Dimensions: 9.3 x 6.3 x 1 inches
• Shipping Weight: 1.5 pounds
• Amazon Best Sellers Rank: #1,657,573 in Books (See Top 100 in Books)