PCR Applications: Protocols for Functional Genomics [Plastic Comb]

Michael A. Innis (Editor), David H. Gelfand (Editor), John J. Sninsky (Editor)

Book Description

ISBN-10: 0123721865 | ISBN-13: 978-0123721860 | Publication Date: May 17, 1999 | Edition: 1

PCR is the most powerful technique currently used in molecular biology. It enables the scientist to quickly replicate DNA and RNA on the benchtop. From its discovery in the early 80's, PCR has blossomed into a method that enables everything from ready mutation of DNA/RNA to speedy analysis of tens of thousands of nucleotide sequences daily.
PCR Applications examines the latest developments in this field. It is the third book in the series, building on the previous publications PCR Protocols and PCR Strategies. The manual discusses techniques that focus on gene discovery, genomics, and DNA array technology, which are contributing factors to the now-occurring bioinformatics boom.

Key Features
* Focuses on gene discovery, genomics, and DNA array technology
* Covers quantitative PCR techniques, including the use of standards and kinetic analysis
includes statistical refinement of primer design parameters
* Ilustrates techniques used in microscopic tissue samples, such as single cell PCR, whole cell PCR, laser capture microdissection, and in situ PCR
Entries provide information on:
* Nomenclature
* Expression
* Sequence analysis
* Structure and function
* Electrophysiology
* Parmacology
* Information retrieval

Editorial Reviews

Review

Praise for PCR Strategies:
"This book succeeds in its goals of providing advanced concepts and procedures for PCR to researchers in both the basic and clinical sciences. This book contains the efforts of a number of laboratories from both industry and academics that are prominent in the field of PCR applications, and it provides a useful companion to the earlier book."
Received 4 Star Rating
--DOODY'S PUBLISHING REVIEWS
Praise for PCR Protocols
"For an up-to-date, definitive discussion of technical variations and the wide range of known applications, PCR Protocols is an invaluable reference... While PCR is in its infancy, this work will certainly be updated many times and by many others, however as a reflection of the scope of PCR to date, it will not be replaced or rendered obsolete."
--APPLIED CYTOGENETICS
"PCR protocols is an excellent lab manual for making PCR work... The breadth of the applications and methodology described in this book should give even the novice the confidence and skill to make PCR work... In summary, if you have questions about PCR, PCR Protocols probably has the answers."
--TRENDS IN GENETICS
"This volume owes its value to the expertise of its contributors, all specialists of international renown in the developing field of PCR... This book is an excellent compilation for all who, having a general background in molecular biology, wish to practice PCR...No user of the PCR technique could wish to miss it."
--TIBTECH

From the Back Cover

The polymerase chain reaction is the most powerful technique currently used in molecular biology laboratories. From its discovery in the early 80s, PCR has blossomed into a method that enables everything from ready mutation of DNA to speedy analysis of tens of thousands of nucleotide sequences daily.
PCR Applications: Protocols for Functional Genomics examines the latest PCR implementations. It complements the other books in the series, PCR Protocols (1990) and PCR Strategies (1995), and techniques discussed are relevant to the now-occurring bioinformatics and proteomics boom.
Key Features:
* focuses on gene discovery, genomics, and DNA array technology
* covers quantitative PCR techniques, including the use of standards and kinetic analysis
* includes statistical refinement of primer design parameters
* illustrates techniques used in microscopic tissue samples, such as single cell PCR, whole cell PCR, laser capture microdissection, and in situ PCR

See all Editorial Reviews

Product Details

• Plastic Comb: 566 pages
• Publisher: Academic Press; 1 edition (May 17, 1999)
• Language: English
• ISBN-10: 0123721865
• ISBN-13: 978-0123721860
• Product Dimensions: 9.2 x 6.9 x 0.9 inches
• Shipping Weight: 1.8 pounds (View shipping rates and policies)
• Average Customer Review: 4.0 out of 5 stars  See all reviews (2 customer reviews)
• Amazon Best Sellers Rank: #2,667,481 in Books (See Top 100 in Books)

Customer Reviews

Most Helpful Customer Reviews

13 of 16 people found the following review helpful:

5.0 out of 5 stars a must-have for a molecular biology lab, June 21, 2000

By A Customer

This review is from: PCR Applications: Protocols for Functional Genomics (Plastic Comb)

I would like to say that this is a great book on the technology of PCR. It provides the principles, applications, modifications and innovations of this technology and is really helpful in any molecular biology lab. (you can bet it offers a lot more than the conventional stuff in the red book, blue book, current protocols and whatsoever) and as a matter of fact, i have the former books of the series such as PCR protocols and PCR strategies, yet i think the book is much worth the money. it is comprehensive and easy to read--you do not have to read it through of course, but it is always a nice help when you hit some problems--so it would be really nice to have a copy of this book in a lab which does PCR often. besides, this book has much new applications of this method, which are currently very hot in the field of biology--like genomics and microarrays. i would like to recommend this book to the colleagues.

0 of 2 people found the following review helpful:

3.0 out of 5 stars Applications, but not very detailed..., August 26, 2002

By 

M. Francois Rosenfeld "rosenfel31" - See all my reviews
(REAL NAME)   

This review is from: PCR Applications: Protocols for Functional Genomics (Plastic Comb)

This book presents a lot of applications for PCR.
It's interesting to read, but sometimes unclear, and it lacks detailed protocols (the one given in each chapter isn't very useful, in general).
The three first chapters are useless, but the rest of the book is still valuable.
Why not buy it, if you already have all the PCR-litterature.