PCR 3: PCR In Situ Hybridization: A Practical Approach [Hardcover]

C. Simon Herrington (Editor), John J. O'Leary (Editor), C.S. Herrington (Author)

Book Description

ISBN-10: 0199636338 | ISBN-13: 978-0199636334 | Publication Date: January 15, 1998 | Edition: 1

PCR 3: In Situ Hybridization is a unique and timely collection of well-tested protocols for the amplification of DNA and RNA in cells and tissues. For each topic covered, leading experts in the field provide detailed guidance on the key steps in the protocols, numerous hints and tips for success, and advice on trouble-shooting. Topics include: DNA in situ PCR and DNA PCR in situ hybridization (PCR ISH) for the detection of DNA targets in cells; reverse trancriptase in situ PCR (RT-PCR) and RT-PCR ISH for the detection of RNA targets; and PRINS (primed in situ synthesis) for chromosomal analysis in interphase nuclei and metaphase chromosome spreads. This book will be invaluable to molecular biologists, pathologists, geneticists, and all those seeking to perform in situ analyses of nucleic acid molecules.

Editorial Reviews

Review

`The purpose of this multiauthor manual is to provide protocols for in-cell amplification and detection of DNA and RNA in cells. Its 9 chapters cover (1) fixation of tissues for the polymerase chain reaction, (2) in situ genetic analysis with selective ultraviolte radiation fractionation, (3) in situ hybridization, (4) plymerase chain reaciton in situ hybridization, (5) reverse transcriptase in situ polymerase chain reaction for RNA detection (6) primed in situ DNA syntehsis, (7) application of in situ polymerase chain reaction techniques to human tisseus, (8) applicationa dn modifications of plymerase chain reaction in situ hybridization, and (9) automation of in situ amplification. There is a subject index, and a list of suppliers of material and equipment.' CAB Abstracts

`'...These techniques have enormous ptential for biologists, pathologists, geneticists, etc. in allowing ready access to any DNA or RNA target within the cell and offer the investigator the ability to perform sub-genomic in situ analyses.'' Biochimica Clinica, 1999, vol. 23, n. 1

About the Author

C. S. Herrington, Senior Lecturer, University of Liverpool. J. J. O'Leary, Doctor, John Radcliffe Hospital, Oxford.

Product Details

• Hardcover: 205 pages
• Publisher: Oxford University Press, USA; 1 edition (January 15, 1998)
• Language: English
• ISBN-10: 0199636338
• ISBN-13: 978-0199636334
• Product Dimensions: 9.3 x 6.3 x 0.8 inches
• Shipping Weight: 1.2 pounds
• Amazon Best Sellers Rank: #9,198,843 in Books (See Top 100 in Books)