A comprehensive collection of state-of-the-art techniques for generating transgenic plants that are resistant to plant viruses via the cloning and expression of the coat protein gene. Its unfailingly reproducible methods, perfected by hands-on masters, cover the entire process from virus isolation, RNA extraction, and cloning coat protein genes, to the introduction of the coat protein gene into the plant genome and the testing of transgenic plants for resistance. Methods for testing for transformation by PCR and Southern blotting, the detection of RNA transcripts by Northern blotting, and the production of protein by Western analysis are provided, as are methods for challenging the transgenic plants produced and for detecting and measuring the levels of virus.
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Plant Virology Protocols offers for the first time a comprehensive collection of state-of-the-art techniques for generating transgenic plants that are resistant to plant viruses via the cloning and expression of the coat protein gene. Its unfailingly reproducible methods, perfected by hands-on masters, cover the entire process from virus isolation, RNA extraction, and cloning coat protein genes, to the introduction of the coat protein gene into the plant genome and the testing of transgenic plants for resistance. Methods for testing for transformation by PCR and Southern blotting, the detection of RNA transcripts by Northern blotting, and the production of protein by Western analysis are provided, as are methods for challenging the transgenic plants produced and for detecting and measuring the levels of virus. The authoritative contributors also discuss the history and mechanisms of coat protein-mediated protection and the key ethical issues surrounding transgenic technology.
Unprecedented in its comprehensiveness and its many time-proven methods, Plant Virology Protocols is certain to become the authoritative standard reference for plant scientists who want to tap the enormous benefits this technology has to offer.
Unprecedented in its comprehensiveness and its many time-proven methods, Plant Virology Protocols is certain to become the authoritative standard reference for plant scientists who want to tap the enormous benefits this technology has to offer.
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First Sentence:
Methods developed for plant virology have been of central importance to other branches of plant pathology. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
sonchus yellow net virus, superinfecting virus, inoculation buffer, laboratory sealing film, barley mild mosaic virus, genomic blot analysis, alfalfa mosaic virus coat protein, aucuba mosaic virus, capsid protein sequence, local lesion assay, triple gene block, sucrose pad, mosaic virus genome, plant reoviruses, pea early browning virus, virus coat protein gene, virus purification, plant rhabdoviruses, density gradient fractionator, virus pellet, mannitol buffer, mosaic potexvirus, plant virus diseases, tobacco streak virus, grinding buffer Key Phrases - Capitalized Phrases (CAPs): (learn more)
Humana Press Inc, Plant Virology Protocols, Transgenic Resistance Edited, Nucleic Acids Res, New York, Plant-Microbe Interact, United Kingdom, Plant Pathol, Boehringer Mannheim, Plant Mol, Taxonomy of Viruses, Boca Raton, Cold Spring Harbor Laboratory, Plant Physiol, Plant Rhabdovirus Purification, Computer Analysis of Amino Acid Sequences, Methods Enzymol, Molecular Analysis of Transgenic Rice, Virus Res, San Diego, Easy Hyb, Plant Mot, Production of Transgenic Rice, Russet Burbank, Association of Applied Biologists New!
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Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Methods developed for plant virology have been of central importance to other branches of plant pathology. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
sonchus yellow net virus, superinfecting virus, inoculation buffer, laboratory sealing film, barley mild mosaic virus, genomic blot analysis, alfalfa mosaic virus coat protein, aucuba mosaic virus, capsid protein sequence, local lesion assay, triple gene block, sucrose pad, mosaic virus genome, plant reoviruses, pea early browning virus, virus coat protein gene, virus purification, plant rhabdoviruses, density gradient fractionator, virus pellet, mannitol buffer, mosaic potexvirus, plant virus diseases, tobacco streak virus, grinding buffer Key Phrases - Capitalized Phrases (CAPs): (learn more)
Humana Press Inc, Plant Virology Protocols, Transgenic Resistance Edited, Nucleic Acids Res, New York, Plant-Microbe Interact, United Kingdom, Plant Pathol, Boehringer Mannheim, Plant Mol, Taxonomy of Viruses, Boca Raton, Cold Spring Harbor Laboratory, Plant Physiol, Plant Rhabdovirus Purification, Computer Analysis of Amino Acid Sequences, Methods Enzymol, Molecular Analysis of Transgenic Rice, Virus Res, San Diego, Easy Hyb, Plant Mot, Production of Transgenic Rice, Russet Burbank, Association of Applied Biologists New!
Books on Related Topics | Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Citations (learn more)
This book cites 47
books:
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14
books
cite this book:
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- Plant Virology, Third Edition by R. E.F. Matthews on 4 pages
- BIOS Instant Notes in Molecular Biology by Phil Turner on 4 pages
- Diagnosis of Plant Virus Diseases by R. E. F. Matthews on page 12, page 142, and page 468
- Virus Taxonomy: VIIIth Report of the International Committee on Taxonomy of Viruses by Claude M. Fauquet on page 96, page 149, and page 180
- The Flaviviruses: Structure, Replication and Evolution, Volume 59 (Advances in Virus Research) by Thomas J. Chambers on page 187, and page 195
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- Virus and Virus-like Diseases of Potatoes and Production of Seed-Potatoes by Gad Loebenstein on page 305, and page 308
- Nmda Receptor Protocols (Methods in Molecular Biology) by Min Li in Front Matter
- 2-D Proteome Analysis Protocols (Methods in Molecular Biology) by Andrew J. Link in Front Matter
- Adhesion Protein Protocols (Methods in Molecular Biology) by Elisabetta Dejana in Front Matter
- Electro Microscopy Methods and Protocols (Methods in Molecular Biology) by M. A. Nasser Hajibagheri in Front Matter
See all 14 books citing this book
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