Plasmids: A Practical Approach (The Practical Approach, No 138) [Paperback]

K. G. Hardy (Editor)

Book Description

ISBN-10: 0199634440 | ISBN-13: 978-0199634446 | Publication Date: February 17, 1994 | Edition: 2

This completely updated and revised second edition of Plasmids: A Practical Approach provides the researcher with detailed protocols for studying bacterial plasmids and for using plasmids and phagemids as vectors. With many new sections and protocols, it is the most up-to-date reference describing the techniques for the study of plasmid replication and maintenance, the fundamental methods for purifying plasmids and introducing them into cells, and the protocols for the mutagenesis and analysis of plasmid-encoded products. The book features further techniques for the study of plasmids and the use of plasmid vectors in such important groups of bacteria as animal and plant pathogens harboring virulence plasmids, Streptomyces, and lactococci. Plus, the book describes the use of plasmid composite vectors for expression of cDNA cloning, and much more. It is a invaluable reference for molecular biologists, microbiologists, and biotechnology researchers. It includes detailed protocols for studying bacterial plasmids and for using both plasmids and phagemids as vectors, techniques for studying fundamental aspects of plasmids, such as replication, are included, as well as methods for using plasmid vectors in important groups of bacteria. Also described are protocols for using phagemids, which are extremely useful tools for genetic engineering.

Editorial Reviews

Review

'this volume provides practical guidance on plasmid replication and maintenance, methods for purifying plasmids and introducing them into cells ... essential reading for all those using recombinant DNA techniques' ASslib Book Guide, Vol. 59, No. 4, April 1994

'this volume provides practical guidance on plasmid replication and maintenance, methods for purifying plasmids and introducing them into cells ... essential reading for all those using recombinant DNA techniques' Aslib Book Guide, Vol. 59, No. 4, April 1994

`The book will be essential reading for all those using recombinant DNA techniques.' Aslib Book Guide, Vol. 59, No. 4, April 1994

About the Author

K. G. Hardy, Assistant Director, Glaxo Institute for Molecular Biology, Geneva.

Product Details

• Paperback: 280 pages
• Publisher: Oxford University Press, USA; 2 edition (February 17, 1994)
• Language: English
• ISBN-10: 0199634440
• ISBN-13: 978-0199634446
• Product Dimensions: 9.1 x 6.1 x 0.6 inches
• Shipping Weight: 4 ounces (View shipping rates and policies)
• Amazon Best Sellers Rank: #4,240,370 in Books (See Top 100 in Books)