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The Polymerase Chain Reaction
 
 
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The Polymerase Chain Reaction [Paperback]

Kary B. Mullis (Editor), Francois Ferre (Editor), Richard A. Gibbs (Editor), J.D. Watson (Foreword)

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Book Description

0817637508 978-0817637507 March 1, 1994 1
Edited by the inventor of polymerase chain reaction (PCR) and the 1993 Nobel Prize winner in Chemistry, Kary Mullis, as well as two experts in the field, this handbook provides up-to-date methodological protocols from the world's leading laboratories, in addition to new techniques and enhanced applications not yet available in book form. Nearly 40 chapters inform the novice and experienced PCR user on how to optimize their results. In the chapters on applications, researchers provide not only protocols, but also descriptions of how PCR has revolutionized their particular field. Future enhancements of PCR, as well as new potential uses, are discussed. Readers can learn how PCR has changed the face of diagnostic testing, cancer research, genetics, forensics, plant biology, DNA sequencing and gene therapy. Special sections include the latest on QPCR, non-isotopic detection, genetic analysis, and PCR and the world of business, which includes a behind-the-scenes look at the legal battles between biotech giants Cetus and DuPont. It also provides insights into the origins of PCR and the history of nucleic acid research.

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Inside This Book (learn more)
First Sentence:
The advent of polymerase chain reaction (PCR) has greatly accelerated the progress of studies on the genomic structure of various organisms, and any region in even highly complex genomes can be specifically amplified in a few hours by the technique, if the flanking nucleotide sequences are known (Saiki et al., 1988). Read the first page
Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
air cycler, mismatch cleavage method, color complementation assay, illegitimate transcripts, proviral copy number, time seg, standard transcript, munodeficiency virus type, stringency cycles, aided transcript titration assay, illegitimate transcription, hybridization protection assay, transcript sequencing, gel isolation, dystrophin transcript, cleotide primers, dystrophin gene, using nested primers, relative quantitation, repertoire analysis, junctional sequences, maize inbred lines, magnetic rack, pooling scheme, reaction assembly
Key Phrases - Capitalized Phrases (CAPs): (learn more)
Proc Natl Acad Sci, Nucl Acids Res, Clin Microbiol, New York, Anal Biochem, Birkhauser Boston, Hum Genet, San Diego, Methods Applic, Academic Press, Infect Dis, Cold Spring Harbor, Mol Cell Probes, Cancer Res, Mol Biol, Virol Methods, Biochem Biophys Res Commun, Biol Chem, Exp Med, Manuel Perucho, Med Virol, Methods Enzymol, Axel Kahn, Clin Chem, Jamel Chelly
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