A Practical Guide to the Study of Calcium in Living Cells, Volume 40 (Methods in Cell Biology, V. 40) [Plastic Comb]

Richard Nuccitelli (Editor), Leslie Wilson (Series Editor), Paul T. Matsudaira (Series Editor)

Book Description

Publication Date: March 24, 1994 | ISBN-10: 0125228104 | ISBN-13: 978-0125228107

A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don'ts and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation.

Key Features
* Describes techniques for detection of [Ca2+]i: Ca2+ - sensitive microelectrodes
* fluorescent dyes
* luminescent proteins
* Includes techniques for perturbing intracellular Ca2+
* Covers detailed methodology plus problems and pitfalls of each technique
* Contains a practical guide to preparing Ca2+ buffers with an easy-to-use computer program
* Color plates illustrate techniques such as
* Confocal ratio-imaging
* Use of aequorin

Editorial Reviews

Review

"This multiauthored book... provides theoretical and applied aspects of different established techniques for the measurement of [Ca²⁺]i at submicromolar concentration. Each chapter is organized, keeping in view the needs of a person working in the laboratory, by giving the detailed methodology required for the implementation of that particular technique, and all the chapters are well referenced...The book provides a pragmatic approach for the estimation of cytosolic [Ca²⁺]i and also provides a means for studying the spatial and temporal resolution of [Ca²⁺]i. The book achieves the stated goal in the preface, 'this material will be very useful to the investigator who wants to apply any of these methods to his or her research."
--RAMESH BHALLA, University of Iowa, Iowa City
"The book achieves the stated goal in the preface, this material will be very useful to the investigator who wants to apply any of these methods to his or her research."
--MOLECULAR & CELLULAR NEUROSCIENCES

From the Back Cover

This volume in the acclaimed series Methods in Cell Biology is designed for students and researchers in biology and biomedicine. A Practical Guide to the Study of Calcium in Living Cells describes highly sensitive techniques for measuring the calcium vital to many important cellular functions. This manual enables investigators to evaluate confocal images, use the latest dyes, and design Ca²⁺ buffers appropriate to their research needs.
Highlights
* Describes techniques to
* Detect Ca²⁺ with sensitive microelectrodes, fluorescent dyes, and luminescent proteins
* Perturb intracellular Ca²⁺
* Prepare Ca²⁺ buffers with an easy-to-use computer program

* Contains color plates that illustrate confocal ratio-imaging and the use of aequorin
* Covers methods in detail and describes the problems and pitfalls of each technique

Product Details

• Plastic Comb: 368 pages
• Publisher: Academic Press (March 24, 1994)
• Language: English
• ISBN-10: 0125228104
• ISBN-13: 978-0125228107
• Product Dimensions: 9.3 x 8.1 x 0.9 inches
• Shipping Weight: 1.9 pounds (View shipping rates and policies)
• Amazon Best Sellers Rank: #4,187,224 in Books (See Top 100 in Books)