Practical Flow Cytometry [Hardcover]

Howard M. Shapiro (Author)

Book Description

ISBN-10: 0471411256 | ISBN-13: 978-0471411253 | Publication Date: March 14, 2003 | Edition: 4

From the reviews of the 3rd Edition...

"The standard reference for anyone interested in understanding flow cytometry technology."
American Journal of Clinical Oncology

"...one of the most valuable of its genre and...addressed to a wide audience?written in such an attractive way, being both informative and stimulating."
Trends in Cell Biology

This reference explains the science and discusses the vast biomedical applications of quantitative analytical cytology using laser-activated detection and cell sorting. Now in its fourth edition, this text has been expanded to provide full coverage of the broad spectrum of applications in molecular biology and biotechnology today. New to this edition are chapters on automated analysis of array technologies, compensation, high-speed sorting, reporter molecules, and multiplex and apoptosis assays, along with fully updated and revised references and a list of suppliers.

Editorial Reviews

Review

"one of the most valuable of its genre and...addressed to a wide audience.... Written in such an attractive way, being both informative and stimulating" (Zbigniew Darzynkiewicz, Trends in Cell Biology)

"...there is no question that this is THE reference book on flow cytometry...You need this book if you use flow cytometry. Get it!" (Doody?s Electronic Journal, October 25, 2003)

"...This complete reference examines the vast biomedical applications?fully updated and revised..." (Jnl. Of Investigational Allergology and Clinical Immunology, vol 13; no 4)

Review

"[Practical Flow Cytometry, Fourth Edition] is one of the best textbooks for graduate students interested in biomedical sciences ... if you can only have one book on flow cytometry, this has to be the one."
—J. Paul Robinson, Ph.D., Professor of Immunopharmacology and Professor of Biomedical Engineering, Purdue University, West Lafayette, Indiana

See all Editorial Reviews

Product Details

• Hardcover: 736 pages
• Publisher: Wiley-Liss; 4 edition (March 14, 2003)
• Language: English
• ISBN-10: 0471411256
• ISBN-13: 978-0471411253
• Product Dimensions: 11.4 x 8.6 x 1.7 inches
• Shipping Weight: 4 pounds (View shipping rates and policies)
• Average Customer Review: 4.2 out of 5 stars  See all reviews (8 customer reviews)
• Amazon Best Sellers Rank: #899,663 in Books (See Top 100 in Books)

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Customer Reviews

Most Helpful Customer Reviews

25 of 27 people found the following review helpful:

5.0 out of 5 stars The definitive reference work on flow, December 12, 1999

By 

Guy Hermans "GeHa" (Ghent, Belgium) - See all my reviews
(REAL NAME)   

This review is from: Practical Flow Cytometry (Hardcover)

This book is quite possibly the most comprehensive reference book on flow cytometry available. Every aspect of a modern cytometer is covered in depth, including the underlying physics and all their practical consequences to the functioning of the machine. While the incredible wealth of information may make this book unsuitable as an introductory manual to the novice, it is indispensable in any lab that uses this technique on a regular basis.

4 of 4 people found the following review helpful:

5.0 out of 5 stars (Flow) Cytometry ultimate reference, textbook, methods and all (including poetry), January 25, 2008

By 

Uriel Trahtemberg "MD/PhD student" (Jerusalem, Israel.) - See all my reviews
(REAL NAME)   

This review is from: Practical Flow Cytometry (Hardcover)

Shapiro's book tries to be everything to everyone and if there ever was a book that succeeded doing so, this is it. Fully referenced (1000's of references) like an academic paper; containing all the information and explanations, from physics principles and implementations; to chemical structures and properties; to biological models and considerations; to practical uses and applications. It also contains an historical introduction that not only adds perspective but also enormous insight into the field. The book is fairly long, and as such maybe is not the best place for absolute beginners in the field. Nonetheless, knowing as much, the author introduced a chapter that is a briefer introduction to the field, allowing beginners to gain an initial solid footing before delving deeper. Anyone who uses flow cytometry on a regular basis, and certainly anyone using flow cytometry as an important research or clinical method should have this book and read it. You can always skip the parts that might not interest you or that are less relevant. In fact, originally I intended to do so, but once you begin to read you can't stop, it's almost like a novel. The author's witty, down to earth and extremely clear prose makes it easier to follow the denser scientific parts and make plain enjoyable and funny the other parts. He even adds poetry and music, showing you what it is to get joy from science. Importantly, as you read and learn about so many fields and areas related to flow cytometry and cytometry in general, you emerge with a much broader understanding of scientific principles and whole areas of scientific knowledge that are of prime importance to any scientist. It expands your horizons and explains the connections and interplay between different disciplines. Yes, it is that good. Shapiro's book is also full with tips, short protocols, ideas and implementations that have proven to be incredibly helpful to my scientific work. The reason my review's title is '(Flow) cytometry...' is that after finishing the book you have really gained insight and knowledge on cytometry in general and not only on flow cytometry. Finally, even if not read cover to cover, this book is the definitive reference for all things (flow) cytometry, and as such has a place of honor in any biologist's library.

1 of 1 people found the following review helpful:

5.0 out of 5 stars Great, February 16, 2008

By 

Forest Evergreen (southern, USA) - See all my reviews

This review is from: Practical Flow Cytometry (Hardcover)

Skimmed it while loading samples as an undergrad researcher. From reading the intro, I guess previous editions actually showed you how to build your own flow cytometer. So for the uber-geek, older editions might be a great find. As for the newer edition, chock full of examples from peer review. Make sure you have a good background in instrumentation before picking it up.