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Practical Molecular Virology (Methods in Molecular Biology)
 
 
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Practical Molecular Virology (Methods in Molecular Biology) [Paperback]

Mary K. Collins (Author)

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Book Description

October 31, 1991 089603299X 978-0896032996 1
Mary K. L. Collins has assembled in Practical Molecular Virology a vanguard collection of readily repeatable methods for gene transfer and expression using a variety of recombinant viral vectors.

In keeping with the established tradition of the series, each technique is presented in an easy-to-follow format designed to work for the novice as well as the seasoned expert. Chapters cover: • life cycles of specific retroviruses and how recombinant vectors are constructed • PCR techniques • poliovirus vectors • herpesvirus vectors • syncytial assays • cell lineage studies • baculovirus and adenovirus vectors • SV40 and EBV vectors • viruses in gene transfer to eukaryotic cells

The wealth of material devoted to recombinant retroviral methods and their applications make Practical Molecular Virology and extremely timely volume, one that will find widespread use throughout biological and biomedical research.

Editorial Reviews

Review

I found the writing style uniformly clear and straightforward throughout....I noticed the well-organized, parallel structure of most chapters, which made them easy to follow and gave evidence of some rather careful editing. Practical Molecular Virology provides a concise, well-organized, well-written...selective overview of eukaryotic viral vectors. As an entry-level text, it surely will assist those wishing to use such vectors to make a choice among established systems. Does this book meet its objective? I believe it does. Methods books are notoriously hard to execute well, but this one succeeds by carefully defining its own niche and then occupying it. -ASM News

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Inside This Book (learn more)
First Sentence:
The discovery of a filterable agent that allowed the transmission of cancers in chicken (1) was the first identification of the viruses now known as retroviruses. Read the first page
Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
gel retention assays, recipient cell line, producer cell line, provirus integration, dyad symmetry element, packaging cell line, cassette vector, vaccinia genome, packaging cells, cellular sequences, poliovirus capsid, retroviral plasmid, molecular virology, selected phenotype, target cell population, extracellular virus, vaccinia virus recombinants, genomic transcripts, insertional activation, virus dilution, infected target cells, test supernatant, producer clones, producer cells, insect cells
Key Phrases - Capitalized Phrases (CAPs): (learn more)
Cold Spring Harbor, Practical Molecular Virology, The Humana Press Inc, Nucleic Acids Res, New York, Institute of Virology, Virus Res, Dulbecco's Modified Eagle's Medium, Laboratory Manual, Coomassie Blue, Nucleic Acid Res, Amersham International, Cancer Res, Mansfield Road, Practical Approach, Sigma Chemical Company, William Heinemann Medical Books
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