The third edition of this established classic text reference builds upon the strengths of its very popular predecessors. Organized as a broadly useful textbook Principles of Fluorescence Spectroscopy, 3rd edition maintains its emphasis on basics, while updating the examples to include recent results from the scientific literature. The third edition includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes and radiative decay engineering. Includes a link to Springer Extras to download files reproducing all book artwork, for easy use in lecture slides. This is an essential volume for students, researchers, and industry professionals in biophysics, biochemistry, biotechnology, bioengineering, biology and medicine.
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Praise for Earlier Editions: "Lakowicz’s Principles of Fluorescence Spectroscopy has been the best one-volume introduction to the biophysical principles of fluorescence methods. - Roger Y. Tsien, Ph.D., Department of Pharmacology and Department of Chemistry and Biochemistry, University of California, San Diego, California "Principles of Fluorescence Spectroscopy is encyclopedic and comprehensive." - Britton Chance, Professor Emeritus in Biochemistry and Biophysics,University of Pennsylvania, School of Medicine, Philadelphia, Pennsylvania "Recommended without reservation both to the novice and to the expert in fluorescence." - Analytical Biochemistry "In addition to its use as a student text, it should be a particularly valuable reference for those involved in biochemical research." - Chemistry in Britain Advance Praise for Third Edition: "This third edition has significantly expanded the topics, and will remain as a leading reference, as well as a text…the information in the book is valuable for a wide range of disciplines." - Robert M. Clegg, Ph.D., Department of Physics, University of Illinois, Champaign-Urbana, Illinois "Overall this is a most welcome, and timely transformation of the classic, and most comprehensive textbook on fluorescence spectroscopy. It should be the number one item on the shopping list for any student or researcher involved in any aspect of fluorescence, be it as a biologist who does some microscopy, or a chemist synthesizing novel fluorophores." - Alan Ryder, Ph.D., National Centre for Biomedical Engineering Science, National University of Ireland-Galway, Galway, Ireland From the reviews of the third edition: "This book gives an overview of the principles and applications of fluorescence. It is well structured, starting with basic knowledge about the phenomena of fluorescence and ending with the latest applications. … highly readable and informative both by novices and by experienced people. … a helpful work of reference and a wonderful creation for learning and teaching. The updated 3rd edition with its appealing design and its absolutely up-to-date and, nevertheless, complete treatment of fluorescence spectroscopy makes it essential for everyone working in this field." (Christiane Albrecht, Analytical and Bioanalytical Chemistry, Vol. 390, 2008)
From the Back Cover
Principles of Fluorescence Spectroscopy, 3rd edition , 3rd edition Joseph R. Lakowicz The third edition of the established classic text reference, Principles of Fluorescence Spectroscopy, will enhance upon the earlier editions' successes. Organized as a textbook for the learning student or the researcher needing to acquire the core competencies, Principles of Fluorescence Spectroscopy, 3e will maintain the emphasis on basics, while updating the examples to include recent results from the literature. The third edition also includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes and radiative decay engineering. This full-color textbook features the following: Problem sets following every chapterGlossaries of commonly used acronyms and mathematical symbols Appendices containing a list of recommended books which expand on various specialized topicsSections describing advanced topics will indicate as such, to allow these sections to be skipped in an introductory course, allowing the text to be used for classes of different levelsIncludes CD-ROM of all figures in a low-res format, perfect for use in instruction and presentations Principles of Fluorescence Spectroscopy, 3rd edition, is an essential volume for students, researchers, and industry professionals in biophysics, biochemistry, biotechnology, bioengineering, biology and medicine. About the Author: Dr. Joseph R. Lakowicz is Professor of Biochemistry at the University of Maryland School of Medicine, Baltimore, and Director of the Center for Fluorescence Spectroscopy. Dr. Lakowicz has published over 400 scientific articles, has edited numerous books, holds 16 issued patents, and is the author of the widely used text, Principles of Fluorescence Spectroscopy now in its 3rd edition.
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6 of 8 people found the following review helpful:
5.0 out of 5 stars
The Luminescence Bible,
By
This review is from: Principles of Fluorescence Spectroscopy (Hardcover)
What's more to say? This is the authoritative, the definitive volume for luminescence experiments from theory to all of the most useful applications. It is readable, dense, useful and indispensable if you do fluorescence, lifetime measurements, energy transfer studies, etc., etc.
1 of 1 people found the following review helpful:
5.0 out of 5 stars
Excellent textbook,
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This review is from: Principles of Fluorescence Spectroscopy (Hardcover)
Nothing to do with rubbish collection of chapters made of friends and friends of friends and me-too sections, called books...
This is an excellent book, as all useful reference books should be, like those of the old times: well written with a unique logic line, uniform notations and plots. Among others, quite low price for the quality of paper and number of pages. I would even say that this is a very good introductory book: it worked well with me, an EE.
1 of 1 people found the following review helpful:
4.0 out of 5 stars
A masterpiece,
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This review is from: Principles of Fluorescence Spectroscopy (Hardcover)
Of all the reference books I have on fluorescence spectroscopy, Dr. Lakowicz's book is the most comprehensive. It is a masterpiece. His book covers many aspects of fluorescent biological systems but it also treats inorganic systems. The foundations of fluorescence and fluorescence spectroscopy are quite comprehensive and very well covered. This is an excellent text and reference book for any person involved not only in bio-related research, but in any discipline involving fluorescence.
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Inside This Book
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Key Phrases - Statistically Improbable Phrases (SIPs):
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global fit, polarized intensity decays, excitation anisotropy spectrum, associated anisotropy decays, anthroyloxy probes, tyrosinate emission, donor decay time, fluorenyl fatty acids, enhanced acceptor emission, spectral relaxation time, complex intensity decays, anisotropy decay analysis, donor intensity decay, excitation anisotropy spectra, general solvent effects, spaced lifetimes, multiple decay times, anisotropy decay law, excitation photoselection, unquenched lifetime, radiative decay engineering, single decay time, tryptophan octyl ester, intensity decay law, phan emission Key Phrases - Capitalized Phrases (CAPs): (learn more)
New York, Phys Chem, American Chemical Society, Anal Chem, Photochem Photobiol, Proc Natl Acad Sci, Chem Phys, Rev Sci Instrum, Anal Biochem, Plenum Press, Chem Soc, Biol Chem, Biochim Biophys Acta, Phis Chem, Methods Enzymol, Elsevier Science, Academic Press, Chem Phis, Nature Biotechnol, Nucleic Acids Res, Appl Spectrosc, Opt Spectrosc, Anal Chim Acta, John Wiley, Biomed Opt Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Surprise Me!
global fit, polarized intensity decays, excitation anisotropy spectrum, associated anisotropy decays, anthroyloxy probes, tyrosinate emission, donor decay time, fluorenyl fatty acids, enhanced acceptor emission, spectral relaxation time, complex intensity decays, anisotropy decay analysis, donor intensity decay, excitation anisotropy spectra, general solvent effects, spaced lifetimes, multiple decay times, anisotropy decay law, excitation photoselection, unquenched lifetime, radiative decay engineering, single decay time, tryptophan octyl ester, intensity decay law, phan emission Key Phrases - Capitalized Phrases (CAPs): (learn more)
New York, Phys Chem, American Chemical Society, Anal Chem, Photochem Photobiol, Proc Natl Acad Sci, Chem Phys, Rev Sci Instrum, Anal Biochem, Plenum Press, Chem Soc, Biol Chem, Biochim Biophys Acta, Phis Chem, Methods Enzymol, Elsevier Science, Academic Press, Chem Phis, Nature Biotechnol, Nucleic Acids Res, Appl Spectrosc, Opt Spectrosc, Anal Chim Acta, John Wiley, Biomed Opt Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Surprise Me!
Citations (learn more)
100
books
cite this book:
See all 100 books citing this book
- Handbook of Biomedical Fluorescence by Mary-Ann Mycek on 9 pages
- Fluorescence Spectroscopy, Volume 278 (Methods in Enzymology) by John N. Abelson on 6 pages
- Biophotonics, Part A, Volume 360 (Methods in Enzymology) by Gerard Marriott on 5 pages
- Introduction to Fluorescence Spectroscopy (Techniques in Analytical Chemistry) by Ashutosh Sharma on 4 pages
- Methods in Cellular Imaging by Ammasi Periasamy on 4 pages
See all 100 books citing this book
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