Principles and Techniques of Electron Microscopy: Biological Applications [Hardcover]

M. A. Hayat (Author)

Book Description

ISBN-10: 0521632870 | ISBN-13: 978-0521632874 | Publication Date: April 13, 2000 | Edition: 4

Principles and Techniques of Electron Microscopy is the standard work for biological electron microscopists wishing to learn how to prepare their specimens for electron microscopic investigation. This fully revised and expanded fourth edition includes three new chapters covering such topics as plant tissues, immunocytochemistry, and applications of microwave irradiation to microscopy. It provides practical instructions on how to process biological specimens, as well as a detailed discussion on the principles underlying the various processes. Dr. Hayat presents methods in a self-explanatory form and includes alternative procedures and points of disagreement to help the reader interpret data accurately. What sets this book apart from its competition is that it not only describes techniques but also explains their fundamental principles; that is, those chemical reactions underlying the use of various reagents for preserving and staining cellular components.

Editorial Reviews

Review

"This beautifully printed new edition, to those experienced in the science and art of electron microscopy, will be like an old friend coming home, but even more prosperous than before and bringing specially selected gifts. Researchers, students, or technical staff who are skilled in the art, and beginners at least introduced to the techniques of electron microscopy and other forms of microscopy will find this book both pleasant and invaluable." Biotechnic Histochemistry

"[A] standard text for any biological electron microscopist intending to prepare tissues for study by the electron microscope...essentially a Handbook for anyone doing EM preparative research...the Hayat book will prove to be essential to every laboratory planning to use 'state of the art' EM techniques in the new millennium." British Society of Developmental Biology (Summer 2001)

"Hayat's latest edition will undoubtedly be the book that is sought by graduate and undergraduate students...Hayat fills 543 pages with details and images on every aspect of biological electron microscopy for both plant and animal tissues...this text is well on its way to becoming the classic introductory teaching book for biological TEM." Microscopy and Microanalysis (4rth ed.)

From reviews of the previous editions...

"This book is to be highly recommended to biological electron microscopists at all levels, because it is the most complete and up-to-date compilation and discussion of 'routine' TEM-procedures, i.e., fixation, embedding, cutting, staining, and support films...gives a starting point for anyone who wishes to know more about any particular specimen preparation procudure for TEM." The Journal of Histochemistry and Cytochemistry (2nd edition)

"...a rewarding educational experience...a good book and well worth acquiring." Bulletin of the Electron Microscopy Society of America (2nd edition)

"...this text is well on its way to becoming the classic introductory teaching book for biological TEM." Microscopy and Microanalysis

Book Description

This revised and expanded 4th edition of Principles and Techniques of Electron Microscopy provides practical instructions on how to process biological specimens, as well as a detailed discussion on the principles underlying the various processes. The book presents methods in a self-explanatory form. Further, alternative procedures and points of disagreement help the reader interpret data accurately. What sets this book apart from its competition is that it not only describes techniques but also explains the principles behind the techniques; that is, chemical reactions underlying the use of various reagents for preserving and staining cellular components.

Product Details

• Hardcover: 564 pages
• Publisher: Cambridge University Press; 4 edition (April 13, 2000)
• Language: English
• ISBN-10: 0521632870
• ISBN-13: 978-0521632874
• Product Dimensions: 10.3 x 8.3 x 1.1 inches
• Shipping Weight: 3.3 pounds
• Average Customer Review: 5.0 out of 5 stars  See all reviews (1 customer review)
• Amazon Best Sellers Rank: #3,106,883 in Books (See Top 100 in Books)

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Customer Reviews

Most Helpful Customer Reviews

2 of 12 people found the following review helpful:

5.0 out of 5 stars labeling with colloidal gold conjugates, June 15, 2001

By 

Egidio Romano (Miami, Florida United States, Instituto Venezolano de Investigaciones Cientificas. Caracas, Venezuela) - See all my reviews

This review is from: Principles and Techniques of Electron Microscopy: Biological Applications (Hardcover)

It is an excellent handbook for electron microscopy. In the section of labeling with protein A / colloidal gold, the author fails to acknowledge the original contribution on the subject: Romano EL and Romano MS: Staphylococcal protein A bound to colloidal gold: a useful reagent to label antigen-antibody sites in electron microscopy. Immunochemistry 14:711-715,1977. I think that the omission is unfair because later works by others are cited in the same section of the book