This comprehensive, practical, and user-friendly manual is designed to be a practical progression of experimental protocols that an inexperienced investigator may follow when embarking on a biochemical or biotechnology project. It will also appeal to experienced researches and graduate students as a benchtop handbook which, without sacrificing scientific concepts, maintains the spirit of experimentation.
Protein Analysis and Purification: Benchtop Techniques
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Editorial Reviews
From the Back Cover
New technologies and the manner in which scientific research is conducted have been fully incorporated into this thoroughly revised second edition of PROTEIN ANALYSIS AND PURIFICATION. Among the many topics introduced to this new edition are the use of robotics in the lab, the impact of the codification of the human genome, functional proteomics, mass spectrometry and the expanding use of bioinformatics as a tool to improve efficiency and productivity. The author has also included an annotated appendix of the educational materials and technical databases available online to enhance the researcher's benchside access to the latest resources. With solid explanations of the science asking the "why" and literally dozens of step-by-step protocols providing the "how", this new edition is a complete resource for the benchside.
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Most Helpful Customer Reviews
13 of 13 people found the following review helpful:
5.0 out of 5 stars
excellent and clearly presented,
By A Customer
This review is from: Protein Chemistry: Benchtop Techniques (Plastic Comb)
This is a model for all protocol lab manuals. It is clear, precise, and well done. Incorporates the experiences of the authors, which are used to perfect the assays and protocols and minimize insufficient results and data. Troubleshooting tips are useful and original. Truly a necessary benchtop reference for the serious researcher.
1 of 1 people found the following review helpful:
5.0 out of 5 stars
Practical Book,
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Amazon Verified Purchase(What's this?)
This review is from: Protein Analysis and Purification: Benchtop Techniques (Hardcover)
Practical book with very easy to follow and concise protocols about protein purification
If you have to purify and analyze proteins but you re not an expert this is the book for you.
1 of 2 people found the following review helpful:
5.0 out of 5 stars
Very useful reference,
By Armando-Malwani "Seeker" (MN, USA) - See all my reviews
This review is from: Protein Analysis and Purification: Benchtop Techniques (Plastic Comb)
Very useful reference for any biochemistry lab. Less bulkier than cold spring harbor manuals and certainly packed with a lot of useful information. This book would be useful for the professional as well as the novice. Detailed protocols included and updated from the previous edition. The section of post translational modifications was the most useful for my purposes. This book can take you from cells to purified protein under most circumstances with the minimum of repetition. I recommend this book particularly for graduate students in biochemistry who deal with protein purification as well as technicians.
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Inside This Book
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First Sentence:
This book is designed to be a practical progression of experimental techniques an investigator may follow when embarking on a protein purification or protein analysis project. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
neuraminidase buffer, cholera neuraminidase, target glycoprotein, renaturation buffer, reducing sample buffer, carboxy side, immunoprecipitated material, disruption buffer, cleavage buffer, destain solution, target protein, stacking gel solution, phosphoamino acid analysis, denaturation buffer, overlay solution, detergent phase, gel pieces, total cell lysate, precast gels, equilibration buffer, glycine solution, separating gel, acrylamide stock solution, gel bed, phosphopeptide mapping Key Phrases - Capitalized Phrases (CAPs): (learn more)
Biol Chem, Anal Biochem, Methods Enzymol, Cell Biol, New York, Proc Natl Acad Sci, Academic Press, Trends Biochem Sci, Anal Chem, Ann Rev Biochem, Cold Spring Harbor, Mol Biol, Nucleic Acids Res, Biochim Biophys Acta, Man Man, Nat Biotechnol, Oxford University Press, Practical Approach, Proteome Research, San Diego, Boca Raton, Life Science Group, Molecular Probes, Appendix Protocol, Arch Biochem Biophys New!
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Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
This book is designed to be a practical progression of experimental techniques an investigator may follow when embarking on a protein purification or protein analysis project. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
neuraminidase buffer, cholera neuraminidase, target glycoprotein, renaturation buffer, reducing sample buffer, carboxy side, immunoprecipitated material, disruption buffer, cleavage buffer, destain solution, target protein, stacking gel solution, phosphoamino acid analysis, denaturation buffer, overlay solution, detergent phase, gel pieces, total cell lysate, precast gels, equilibration buffer, glycine solution, separating gel, acrylamide stock solution, gel bed, phosphopeptide mapping Key Phrases - Capitalized Phrases (CAPs): (learn more)
Biol Chem, Anal Biochem, Methods Enzymol, Cell Biol, New York, Proc Natl Acad Sci, Academic Press, Trends Biochem Sci, Anal Chem, Ann Rev Biochem, Cold Spring Harbor, Mol Biol, Nucleic Acids Res, Biochim Biophys Acta, Man Man, Nat Biotechnol, Oxford University Press, Practical Approach, Proteome Research, San Diego, Boca Raton, Life Science Group, Molecular Probes, Appendix Protocol, Arch Biochem Biophys New!
Books on Related Topics | Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Citations (learn more)
This book cites 36
books:
See all 36 books this book cites
- Current Protocols in Molecular Biology by Frederick M. Ausubel on 4 pages
- Proteome Research: New Frontiers in Functional Genomics (Principles and Practice) by Marc R. Wilkins on page 115, page 254, and page 319
- Gel Electrophoresis of Proteins: A Practical Approach by B. D. Hames on page 115, page 117, and page 209
- Getting Started by Richard D. Smith on page 117, and page 134
- Antibodies by Edward Harlow on page 210, and page 383
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