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Protein Crystallization (Iul Biotechnology Series) [Hardcover]

Terese M. Bergfors
4.0 out of 5 stars  See all reviews (1 customer review)


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Hardcover, February 1, 1999 --  

Book Description

February 1, 1999 0963681753 978-0963681751 1
Completely revised and updated, Protein Crystallization, 2nd Edition is a greatly expanded follow-up to the best-selling 1st edition. Completely new chapters on high-throughput methods, mass spectrometry, microcalorimetry, counterdiffusion, heavy-atom derivatization, selenomethionine-labeling, rational strategies for crystallization, and protein modification to improve crystallization. Updated chapters on formulation of the protein before crystallization, characterization of the protein by dynamic light scattering, classic methods and the phase diagram, seeding, and cryoprotection of the crystals. Laboratory exercises are included which can be used for teaching purposes in protein crystallization or crystallography courses. The exercises have been tested in the Practical Protein Crystallization courses that have been running since 1994 at Uppsala University (Sweden), as well as courses elsewhere. The book is divided into four separate sections: Methods, Tools and Strategies, Laboratory Exercises, and an A-Z Glossary. There are 30 color plates for identifying phenomena found in crystallization drops. As in the first edition, the emphasis of the book is on practical problems and questions encountered at the lab bench.
--This text refers to an alternate Hardcover edition.

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Editorial Reviews

Review

"This is an essential handbook for anyone engaged in crystallization of macromolecules. It is exceptionally well organized and illustrated and has contributions from all the leaders in what continues to be a challenging and critically important field." --Dr. William L. Duax, American Crystallographic Association, Executive Officer

"Why is crystallization important? At different times, different aspects of protein crystallography have assumed the role of bottleneck. Thus at one time the bottleneck consisted of the lack of a methodology for structure determination, even if a sufficient number of diffraction intensitities were available for structure solution. With the advent of powerful methods for structure solution, the bottleneck shifted to the difficulty in obtaining crystals in sufficient number and of suitable quality to do the diffraction experiment. The present book is dedicated to the problem of describing current methods for protein crystallization and describing them as clear laboratory protocols." --Dr. Herbert A. Hauptman, 1985 Nobel Laureate in Chemistry

"I used the first edition as a graduate student. It was a perfect guide for protein crystallizers at all stages of their careers, providing methodical, clear explanations in lay terminology." --Dr. Lata Govada, Imperial College London --This text refers to an alternate Hardcover edition.

From the Back Cover

Completely revised and updated, Protein Crystallization, 2nd Edition is a greatly expanded follow-up to the best-selling 1st edition. No previous background in crystallization is assumed. Contents include:

--Completely new chapters on high-throughput methods, mass spectrometry, microcalorimetry, counterdiffusion, heavy-atom derivatization, selenomethionine-labeling, rational strategies for crystallization, and protein modification to improve crystallization.
--Updated chapters on formulation of the protein before crystallization, characterization of the protein by dynamic light scattering, classic methods and the phase diagram, seeding, and cryoprotection of the crystals.
--Thirty full-color plates for evaluating crystallization drops.
--Separate section of laboratory exercises, ideal for crystallization courses.
--A-Z glossary. --This text refers to an alternate Hardcover edition.


Product Details

  • Series: Iul Biotechnology Series
  • Hardcover: 306 pages
  • Publisher: Intl Univ Line; 1 edition (February 1, 1999)
  • Language: English
  • ISBN-10: 0963681753
  • ISBN-13: 978-0963681751
  • Product Dimensions: 9.1 x 6.2 x 1.1 inches
  • Shipping Weight: 1.4 pounds
  • Average Customer Review: 4.0 out of 5 stars  See all reviews (1 customer review)
  • Amazon Best Sellers Rank: #3,380,586 in Books (See Top 100 in Books)

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4.0 out of 5 stars Very useful book June 3, 2013
Format:Hardcover|Verified Purchase
The book has been very useful for different level students because is very practice and undestable.
I really can recommend it for study.
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