Methods widely used in the study of DNA binding proteins are presented in this volume. These include purification and protein characterization, assays of protein-DNA binding and protein-induced bending, and biochemical and genetic methods for probing the structure, energy, and specificity of protein-DNA interactions.
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"The Methods in Enzymology series represents the gold-standard."
--NEUROSCIENCE
"Incomparably useful."
--ANALYTICAL BIOCHEMISTRY
"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page."
--BIO/TECHNOLOGY
"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection."
--CHEMISTRY IN INDUSTRY
"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced."
--AMERICAN SOCIETY OF MICROBIOLOGY NEWS
"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work."
--ENZYMOLOGIA
"A series that has established itself as a definitive reference for biochemists."
--JOURNAL OF CHROMATOGRAPHY
"The Methods in Enzymology series represents the gold-standard."
--NEUROSCIENCE
"Incomparably useful."
--ANALYTICAL BIOCHEMISTRY
"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page."
--BIO/TECHNOLOGY
"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection."
--CHEMISTRY IN INDUSTRY
"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced."
--AMERICAN SOCIETY OF MICROBIOLOGY NEWS
"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work."
--ENZYMOLOGIA
"A series that has established itself as a definitive reference for biochemists."
--JOURNAL OF CHROMATOGRAPHY
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Inside This Book
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First Sentence:
One of the important early steps in purifying a DNA-binding protein is to separate the protein of interest from cellular nucleic acids. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
consensus base pair, rapid equilibrium limit, average binding density, permanganate pattern, amino acid under study, cleavage loci, nucleoside technique, binding density function, missing nucleoside experiment, hydroxyl radical cleavage reaction, streptomycin selection, diminished cleavages, extension probing, lactose repressor protein, mobility shift gel, reporter promoter, binding and bending, hydroxyl radical footprints, tract bend, arc gene, favorable contacts, nucleosome reconstitution, substituted protein, cro protein, affinity cleaving Key Phrases - Capitalized Phrases (CAPs): (learn more)
Nucleic Acids Res, New York, Cold Spring Harbor, Academic Press, Genes Deu, Experimental Methods, Laboratory Manual, Molecular Cloning, National Institutes of Health, Practical Approach, San Francisco, Trends Biochem, Case Three, Complex Exists, Molecular Genetics, New England Biolabs, Reagent Protein, San Diego, United States Biochemical, University of Wisconsin-Madison New!
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Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
One of the important early steps in purifying a DNA-binding protein is to separate the protein of interest from cellular nucleic acids. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
consensus base pair, rapid equilibrium limit, average binding density, permanganate pattern, amino acid under study, cleavage loci, nucleoside technique, binding density function, missing nucleoside experiment, hydroxyl radical cleavage reaction, streptomycin selection, diminished cleavages, extension probing, lactose repressor protein, mobility shift gel, reporter promoter, binding and bending, hydroxyl radical footprints, tract bend, arc gene, favorable contacts, nucleosome reconstitution, substituted protein, cro protein, affinity cleaving Key Phrases - Capitalized Phrases (CAPs): (learn more)
Nucleic Acids Res, New York, Cold Spring Harbor, Academic Press, Genes Deu, Experimental Methods, Laboratory Manual, Molecular Cloning, National Institutes of Health, Practical Approach, San Francisco, Trends Biochem, Case Three, Complex Exists, Molecular Genetics, New England Biolabs, Reagent Protein, San Diego, United States Biochemical, University of Wisconsin-Madison New!
Books on Related Topics | Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Citations (learn more)
This book cites 21
books:
See all 21 books this book cites
1
book
cites this book:
- Experiments in Molecular Genetics by Jeffrey H. Miller on page 582, page 604, and page 644
- Molecular Cloning: A Laboratory Manual, Third Edition (3 volume set) by Joe Sambrook on page 75, page 309 (1), and page 309 (2)
- Electron Microscopy in Molecular Biology: A Practical Approach (Practical Approach Series) by J. Sommerville on page 75 (1), page 75 (2), and page 75 (3)
- Detoxication and Drug Metabolism: Conjugation and Related Systems, Volume 77: Volume 77: Detoxification and Drug Metabolism (Methods in Enzymology) by Nathan P. Kaplan in Front Matter (1), and Front Matter (2)
- Nucleic Acids (Methods in Molecular Biology) by John M. Walker on page 15, and page 309
See all 21 books this book cites
- Nucleic Acids: Structures, Properties, and Functions by Victor A. Bloomfield on page 737
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