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Protein Engineering: Principles and Practice [Hardcover]

Jeffrey L. Cleland , Charles S. Craik

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Book Description

February 7, 1996 0471103543 978-0471103547 1
PROTEIN ENGINEERING Principles and Practice

Edited by JEFFREY L. CLELAND CHARLES S. CRAIK

Proteins are involved in every aspect of life-structure, motion, catalysis, recognition and regulation. Protein Engineering: Principles and Practice provides a basic framework for understanding both proteins and protein engineering. This comprehensive book covers general, yet essential knowledge required for successful protein engineering, including everything from the fundamentals to modifying existing proteins and developing new proteins.

The book begins by introducing the main concepts of protein engineering, including: understanding protein conformation, comprehending the relationship between protein composition and structure, and potential methods for predicting a protein's conformation.

Other major subjects addressed are:
* Using different host cell expression systems to produce specific proteins
* Protein folding
* Structure and function of proteins in relation to drug design
* Construction of synthetic metal binding sites in proteins
* Manufacture of tissue plasminogen activator
* Generation of therapeutic antibodies

This broad range of topics provides a solid foundation in protein engineering and supplies readers with knowledge essential to the design and production of proteins.

Of primary interest to protein scientists-both students and researchers, in academia as well as industry-Protein Engineering is also extremely useful to chemical engineers, protein chemists, biochemists, and pharmaceutical chemists.

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Editorial Reviews

From the Publisher

This up-to-the-minute text presents a systematic approach to protein engineering from both fundamental and operational perspectives. Contains a thorough introduction to protein biophysics via chapters dealing with experimental and theoretical determination of protein structure. Discusses each of the various expression systems currently available along with their advantages and limitations. Also includes the difficulty in achieving correctly folded and functional proteins, specific examples of protein engineering use in construction of synthetic metal binding sites, the manufacture of tissue plasminogen and the generation of therapeutic antibodies.

From the Back Cover

PROTEIN ENGINEERING Principles and Practice

Edited by JEFFREY L. CLELAND CHARLES S. CRAIK

Proteins are involved in every aspect of life-structure, motion, catalysis, recognition and regulation. Protein Engineering: Principles and Practice provides a basic framework for understanding both proteins and protein engineering. This comprehensive book covers general, yet essential knowledge required for successful protein engineering, including everything from the fundamentals to modifying existing proteins and developing new proteins.

The book begins by introducing the main concepts of protein engineering, including: understanding protein conformation, comprehending the relationship between protein composition and structure, and potential methods for predicting a protein's conformation.

Other major subjects addressed are:
* Using different host cell expression systems to produce specific proteins
* Protein folding
* Structure and function of proteins in relation to drug design
* Construction of synthetic metal binding sites in proteins
* Manufacture of tissue plasminogen activator
* Generation of therapeutic antibodies

This broad range of topics provides a solid foundation in protein engineering and supplies readers with knowledge essential to the design and production of proteins.

Of primary interest to protein scientists-both students and researchers, in academia as well as industry-Protein Engineering is also extremely useful to chemical engineers, protein chemists, biochemists, and pharmaceutical chemists.

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