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Protein Localization by Fluorescence Microscopy: A Practical Approach
 
 
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Protein Localization by Fluorescence Microscopy: A Practical Approach [Paperback]

Victoria J. Allan (Editor)

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Book Description

February 15, 2000 0199637407 978-0199637409 1
There are an ever-increasing number of genes that have been sequenced but are of completely unknown function. The ability to determine the location of such gene products within the cell, either by the use of antibodies or by the production of chimeras with green fluorescent protein, is a vital step towards understanding what they do. This is one major reason why fluorescence microscopy is enjoying a revival. This no-nonsense guide provides detailed, practical advice on all aspects of the subject: from choosing the right equipment, to interpreting results. It balances the advantages of a wide range of techniques - including live cell work - against the potential pitfalls, offering invaluable "tricks of the trade" along the way. Protein Localization by Fluorescence Light Microscopy: A Practical Approach has something to offer all microscopists, giving a solid grounding to the novice while extending the range of the experienced user.

Editorial Reviews

Review

" ... a useful bencjh-aid. The Editor-Victoria Allan - ans all of the contributors should be congratulated on putting together a valuable reference text " Trends Cell Biology

About the Author

Viki Allan is at University of Manchester.

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Inside This Book (learn more)
First Sentence:
Immunofluorescence on fixed samples forms a vital part of most investigations into protein function, since it provides an idea of the whereabouts of a protein within the cell, which in turn can give important clues as to the protein's function. Read the first page
Key Phrases - Capitalized Phrases (CAPs): (learn more)
Cell Biol, Academic Press, Molecular Probes, San Diego, Cell Sci, Roche Diagnostics, New York, Plant Journal, Santa Cruz, Cold Spring Harbor Laboratory Press, Journal of Microscopy, Natl Acad, Practical Approach, Agar Scientific Ltd, Continued Method, Laboratory Manual, Oxford University Press, Texas Red, Laboratory Handbook, Plenum Press, Stuart Scientific, Applied Precision Inc, Biomedicals Inc, Current Biology, Silicon Graphics
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