This volume provides a compilation of recent methods for studying protein phosphorylation.
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Editorial Reviews
Review
Praise for the Series
"The Methods in Enzymology series represents the gold-standard."
--NEUROSCIENCE
"Incomparably useful."
--ANALYTICAL BIOCHEMISTRY
"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page."
--BIO/TECHNOLOGY
"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection."
--CHEMISTRY IN INDUSTRY
"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced."
--AMERICAN SOCIETY OF MICROBIOLOGY NEWS
"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work."
--ENZYMOLOGIA
"A series that has established itself as a definitive reference for biochemists."
--JOURNAL OF CHROMATOGRAPHY
"The Methods in Enzymology series represents the gold-standard."
--NEUROSCIENCE
"Incomparably useful."
--ANALYTICAL BIOCHEMISTRY
"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page."
--BIO/TECHNOLOGY
"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection."
--CHEMISTRY IN INDUSTRY
"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced."
--AMERICAN SOCIETY OF MICROBIOLOGY NEWS
"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work."
--ENZYMOLOGIA
"A series that has established itself as a definitive reference for biochemists."
--JOURNAL OF CHROMATOGRAPHY
From the Back Cover
Tony Hunter, Molecular Biology and Virology Laboratory, The Salk Institute, San Diego, California, U.S.A.
Bartholomew M. Sefton, Molecular Biology and Virology Laboratory, The Salk Institute, San Diego, California, U.S.A.
Bartholomew M. Sefton, Molecular Biology and Virology Laboratory, The Salk Institute, San Diego, California, U.S.A.
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Most Helpful Customer Reviews
4.0 out of 5 stars
think you have a signalling cascade? get this!,
By wormspermgrrl (tallahasee,, FL USA) - See all my reviews
This review is from: Protein Phosphorylation, Part B: Analysis of Protein Phosphorylation, Protein Kinase Inhibitors, and Protein Phosphatases, Volume 201: Volume 201: ... Part B (Methods in Enzymology) (Hardcover)
This book has given me so many different approaches to examine my system and the phosphorylation events involved. this series is always good about specificity of the techniques. for instance, it talks about how to design the best amino acid sequence for testing for phosphorylation with gamma ATP. pick this up whenever you can afford it!
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Inside This Book
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First Sentence:
A fast, sensitive, high-performance liquid chromatography (HPLC) method for analyzing the products of protein kinase-catalyzed phosphorylations of the three hydroxyamino acids, tyrosine, serine, and threonine, has been developed. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
preparatory plate, phospho isomers, phosphoserine determination, phospho chromatography buffer, pseudosubstrate structures, monophosphate disodium salt, dithiothreitol adduct, peptide deprotection, phosphoamino acid content, methyl phosphate groups, argon dry, dephosphorylated derivatives, phosphoamino acid analysis, phosphoamino acids, pseudosubstrate region, phosphate acceptor site, phosphorylation stoichiometry, hydrolysis vessel, cellulose side, phosphotyrosyl proteins, diphenyl phosphorochloridate, imidazole hydrochloride, dephospho forms, pseudosubstrate peptides, gel bits Key Phrases - Capitalized Phrases (CAPs): (learn more)
Academic Press, Applied Biosystems, New York, Cold Spring Harbor, Cell Biol, Coomassie Blue, San Diego, Affi-Gel Blue, Cancer Res, New England Nuclear, Pause Argon, Tetrahedron Lett, Foster City, Nucleic Acids Res, Trends Biochem, New Zealand White, Van Patten, Bromphenol Blue, Palo Alto, Pause Load, Protein Res, Separations Group, Speed Vac, Boca Raton, Costa Mesa New!
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Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
A fast, sensitive, high-performance liquid chromatography (HPLC) method for analyzing the products of protein kinase-catalyzed phosphorylations of the three hydroxyamino acids, tyrosine, serine, and threonine, has been developed. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
preparatory plate, phospho isomers, phosphoserine determination, phospho chromatography buffer, pseudosubstrate structures, monophosphate disodium salt, dithiothreitol adduct, peptide deprotection, phosphoamino acid content, methyl phosphate groups, argon dry, dephosphorylated derivatives, phosphoamino acid analysis, phosphoamino acids, pseudosubstrate region, phosphate acceptor site, phosphorylation stoichiometry, hydrolysis vessel, cellulose side, phosphotyrosyl proteins, diphenyl phosphorochloridate, imidazole hydrochloride, dephospho forms, pseudosubstrate peptides, gel bits Key Phrases - Capitalized Phrases (CAPs): (learn more)
Academic Press, Applied Biosystems, New York, Cold Spring Harbor, Cell Biol, Coomassie Blue, San Diego, Affi-Gel Blue, Cancer Res, New England Nuclear, Pause Argon, Tetrahedron Lett, Foster City, Nucleic Acids Res, Trends Biochem, New Zealand White, Van Patten, Bromphenol Blue, Palo Alto, Pause Load, Protein Res, Separations Group, Speed Vac, Boca Raton, Costa Mesa New!
Books on Related Topics | Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Citations (learn more)
This book cites 21
books:
See all 21 books this book cites
- Peptides: Special Methods in Peptide Synthesis v.2: Analysis, Synthesis, Biology (Vol 2) by E. Gross on 4 pages
- Peptides and Protein Phosphorylation by Bruce E. Kemp on page 288, page 294, and page 305
- Detoxication and Drug Metabolism: Conjugation and Related Systems, Volume 77: Volume 77: Detoxification and Drug Metabolism (Methods in Enzymology) by Nathan P. Kaplan in Front Matter (1), and Front Matter (2)
- Selected Methods in Cellular Immunology by Barbara B. Mishell on page 46, and page 83
- Antibodies by Edward Harlow on page 68, and page 268
See all 21 books this book cites
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