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Protein Purification: Principles, High-Resolution Methods, and Applications
 
 
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Protein Purification: Principles, High-Resolution Methods, and Applications [Hardcover]

Jan-Christer Janson (Editor), Lars Rydén (Editor)

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There is a newer edition of this item:
Protein Purification: Principles, High Resolution Methods, and Applications (Methods of Biochemical Analysis) Protein Purification: Principles, High Resolution Methods, and Applications (Methods of Biochemical Analysis)
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Book Description

0471186260 978-0471186267 February 27, 1998 2
Praise for the First Edition of Protein Purification:
"Contains much useful information for both the novice in protein purification and the experienced practitioner . . . [an] excellent practical manual." -Trends in Analytical Chemistry.
"[This book is] easy to read and gives a comprehensive description of all major methods of protein purification . . . a much welcomed source of information for protein biochemists." -FEBS Letters.
This thoroughly revised and updated edition of Protein Purification provides state-of-the-art coverage of chromatographic and electrophoretic protein separation and characterization methods. Balancing theory, procedures, and applications, it offers professionals and students in biochemistry, organic chemistry, and analytical chemistry quick access to a wide range of important techniques, including:
* Gel filtration and ion exchange chromatography
* Affinity and covalent chromatography
* Reversed phase and hydrophobic interaction chromatography
* Electrophoresis in gels
* Immunoelectrophoresis and capillary electrophoresis
* Protein mapping by two-dimensional polyacrylamide gel electrophoresis
* Protein elution and blotting methods
* Large-scale electrophoresis processes.

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Editorial Reviews

From the Back Cover

Praise for the First Edition of Protein Purification:

"Contains much useful information for both the novice in protein purification and the experienced practitioner . . . [an] excellent practical manual." --Trends in Analytical Chemistry.

"[This book is] easy to read and gives a comprehensive description of all major methods of protein purification . . . a much welcomed source of information for protein biochemists." --FEBS Letters.

This thoroughly revised and updated edition of Protein Purification provides state-of-the-art coverage of chromatographic and electrophoretic protein separation and characterization methods. Balancing theory, procedures, and applications, it offers professionals and students in biochemistry, organic chemistry, and analytical chemistry quick access to a wide range of important techniques, including:
* Gel filtration and ion exchange chromatography
* Affinity and covalent chromatography
* Reversed phase and hydrophobic interaction chromatography
* Electrophoresis in gels
* Immunoelectrophoresis and capillary electrophoresis
* Protein mapping by two-dimensional polyacrylamide gel electrophoresis
* Protein elution and blotting methods
* Large-scale electrophoresis processes.

About the Author

JAN-CHRISTER JANSON is Adjunct Professor of Technical Biochemistry at Uppsala University and Principal Scientist at Amersham Pharmacia Biotech in Sweden.
LARS RYDEN is Assistant Professor of Biochemistry at Uppsala University in Sweden.

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Inside This Book (learn more)
First Sentence:
The development of techniques and methods for the separation and purification of biological macromolecules such as proteins has been an important prerequisite for many of the advancements made in bioscience and biotechnology over the past three decades. Read the first page
Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
alifatic thiol, forced flow electrophoresis, recycling electrophoresis, thiol gels, reductive elution, titration curve analysis, disulfide oxides, chromatographic contact region, covalent chromatography, protein surface charge, focusing buffer, solubilizing mixture, affinity chromatography adsorbents, analytical gel filtration, sample applicator, lauryl maltoside, carrier ampholytes, solid support media, thiol peptides, beaded agarose, preparative gel filtration, affinity elution, breakthrough peak, intermediate gel, reactive disulfides
Key Phrases - Capitalized Phrases (CAPs): (learn more)
Pharmacia Biotech, New York, Academic Press, Methods Enzymol, Marcel Dekker, High-Resolution Methods, Second Edition, Phenyl Sepharose, Sepharose Fast Flow, Acta Chem, Pergamon Press, American Chemical Society, Boca Raton, Walter de Gruyter, John Wiley, Pharmacia Synthetic, Practical Procedures, Sepharose High Performance, Applied Biosystems, Bio-Rad Laboratories, Blue Sepharose, Merck Synthetic, North Holland, Protides of Biological Fluids, Developments Limited
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