Proteins are an integral part of molecular and cellular structure and function and are probably the most purified type of biological molecule. In order to elucidate the structure and function of any protein it is first necessary to purify it. Protein purification techniques have evolved over the past ten years with improvements in equipment control, automation, and separation materials, and the introduction of new techniques such as affinity membranes and expanded beds. These developments have reduced the workload involved in protein purification, but there is still a need to consider how unit operations linked together to form a purification strategy, which can be scaled up if necessary. The two Practical Approach books on protein purification have therefore been thoroughly updated and rewritten where necessary. The core of both books is the provision of detailed practical guidelines aimed particularly at laboratory scale purification. Information on scale-up considerations is given where appropriate. The books are not comprehensive but do cover the major laboratory techniques and common sources of protein. Protein Purification Techniques focuses on unit operations and analytical techniques. It starts with an overview of purification strategy and then covers initial extraction and clarification techniques. The rest of the book concentrates on different purification methods with the emphasis being on chromatography. The final chapter considers general scale-up considerations. Protein Purification Applications describes purification strategies from common sources: mammalian cell culture, microbial cell culture, milk, animal tissue, and plant tissue. It also includes chapters on purification of inclusion bodies, fusion proteins, and purification for crystallography. A purification strategy that can produce a highly pure single protein from a crude mixture of proteins, carbohydrates, lipids, and cell debris to is a work of art to be admired. These books (available individually or as a set)are designed to give the laboratory worker the information needed to undertake the challenge of designing such a strategy.
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Simon Roe is at AEA Technology Products and Systems, Biosciences, Harwell.
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1 of 1 people found the following review helpful:
4.0 out of 5 stars
Decent Book,
This review is from: Protein Purification Techniques: A Practical Approach (Practical Approach Series) (Paperback)
I was hoping for more detail, but it seems to be a good book for those just learning about the topic of protein purification.
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Inside This Book
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Practical approaches to protein purification contains detailed practical information on separations techniques and the chapters of Protein purification techniques cover the unit operations and analytical techniques involved in some detail while Protein purification applications provides details of how to approach purification from a selected number of typical sources. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
adsorptive chromatography, protein purification applications, covalent chromatography, disc stack centrifuge, adsorption buffer, disruption methods, settling distance, concentration polarization, regeneration solution, equilibration buffer, subsequent purification steps, disruption process, transmembrane pressure, affinity adsorbents, bead mill, operating flow rate, fractionation range, coupling buffer, gel volumes, biomass content, clarification step, isoelectric precipitation, permeate flux, absorbance coefficient, cell disruption Key Phrases - Capitalized Phrases (CAPs): (learn more)
New York, Academic Press, Pharmacia Biotech, John Wiley, Publishers Inc, Marcel Dekker, Coomassie Blue, Coomassie Brilliant Blue, Amersham Pharmacia, Introduction Proteins, Mauri Pinnacle Yeasts, Sepharose Fast Flow New!
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Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Practical approaches to protein purification contains detailed practical information on separations techniques and the chapters of Protein purification techniques cover the unit operations and analytical techniques involved in some detail while Protein purification applications provides details of how to approach purification from a selected number of typical sources. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
adsorptive chromatography, protein purification applications, covalent chromatography, disc stack centrifuge, adsorption buffer, disruption methods, settling distance, concentration polarization, regeneration solution, equilibration buffer, subsequent purification steps, disruption process, transmembrane pressure, affinity adsorbents, bead mill, operating flow rate, fractionation range, coupling buffer, gel volumes, biomass content, clarification step, isoelectric precipitation, permeate flux, absorbance coefficient, cell disruption Key Phrases - Capitalized Phrases (CAPs): (learn more)
New York, Academic Press, Pharmacia Biotech, John Wiley, Publishers Inc, Marcel Dekker, Coomassie Blue, Coomassie Brilliant Blue, Amersham Pharmacia, Introduction Proteins, Mauri Pinnacle Yeasts, Sepharose Fast Flow New!
Books on Related Topics | Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
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- Analytical Chemistry by Hughes on page 239
- Bioanalytical Chemistry by Andreas Manz on page 188
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