Protein Sequencing and Identification Using Tandem Mass Spectrometry (Hardcover)

by Michael Kinter (Author), Nicholas E. Sherman (Author) "Figure 2.1 shows the generalized structure of an amino acid, which is the basic component of protein structure..." (more)
Key Phrases: protein sequencing experiments, product ion spectrum, angiotensin standard, Rapid Comm, Nucleic Acids Res, Mass Spectf (more...)

Editorial Reviews

Review
"[the authors] present their own laboratory-tested protocols for the analysis of a wide variety of samples, providing sufficient detail for duplication of their methods..." (SciTech Book News Vol. 25, No. 2 June 2001)

"...an informative, well-written and well-illustrated book with a large number of useful references.... It should prove invaluable to anyone working in the field of protein sequencing." (Spectroscopyeurope.com)

"[the authors] present...laboratory-tested protocols for the analysis of a wide variety of samples, providing sufficient detail for duplication of their methods..." -- SciTech Book News Vol. 25, No. 2 June 2001

..."the book does a creditable job of making detailed lab protocols accessible ...." -- The Analyst , September 2002

Product Description
How to design, execute, and interpret experiments for protein sequencing using mass spectrometry

The rapid expansion of searchable protein and DNA databases in recent years has triggered an explosive growth in the application of mass spectrometry to protein sequencing. This timely and authoritative book provides professionals and scientists in biotechnology research with complete coverage of procedures for analyzing protein sequences by mass spectrometry, including step-by-step guidelines for sample preparation, analysis, and data interpretation.

Michael Kinter and Nicholas Sherman present their own high-quality, laboratory-tested protocols for the analysis of a wide variety of samples, demonstrating how to carry out specific experiments and obtain fast, reliable results with a 99uccess rate. Readers will get sufficient experimental detail to apply in their own laboratories, learn about the proper selection and operation of instruments, and gain essential insight into the fundamental principles of mass spectrometry and protein sequencing. Coverage includes:

* Peptide fragmentation and interpretation of product ion spectra
* Basic polyacrylamide gel electrophoresis
* Preparation of protein digests for sequencing experiments
* Mass spectrometric analysis using capillary liquid chromatography
* Techniques for protein identification by database searches
* Characterization of modified peptides using tandem mass spectrometry

And much more

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Product Details

• Hardcover: 320 pages
• Publisher: Wiley-Interscience; 1 edition (September 18, 2000)
• Language: English
• ISBN-10: 0471322490
• ISBN-13: 978-0471322498
• Product Dimensions: 9.3 x 6.1 x 0.8 inches
• Shipping Weight: 1.2 pounds (View shipping rates and policies)
• Average Customer Review: 5.0 out of 5 stars See all reviews (3 customer reviews)
• Amazon.com Sales Rank: #440,751 in Books (See Bestsellers in Books)

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Customer Reviews

Most Helpful Customer Reviews

4 of 4 people found the following review helpful:

5.0 out of 5 stars Broad, thorough introduction, November 4, 2003

By	wiredweird "wiredweird" (Earth, or somewhere nearby) - See all my reviews (TOP 100 REVIEWER)

I'm not a bench chemist, but I needed a quick survey of how mass spectroscopy is used in handling proteins and other big biomolecules. This book was it.

Although brief, it is thorough and well-organized. The first two chapters are mostly an introduction. Chapter 1 states the problem being solved. The next chapter briefly introduces older technologies, including chemical techiques and 60s-80s mass spec technique. The next two chapters summarize modern mass spec hardware, then start to show how proteins behave in the environment inside the instrument. That gives the fundamentals of protein sequencing: how the molecules break down, and how the fragments help recreate the molecule. The authors go through a few examples in detail, starting from a mass spectrogram and moving forward to sequence. I was especially impressed by the examples that fail. Mass spec analysis is not a magic wand for producing sequences, it is a deductive process, and can not complete an analysis when clues are missing or ambiguous.

The next three chapters are not about mass spec directly. Instead, they discuss how samples are prepared for analysis. This includes the clearest, most informative description of gel electrophoresis that I've seen, along with features of gel chemistry that do or do not interfere with mass spec measurements. This includes a discussion of protein digests, enzymatically produced fragments, and their place in analysis. I would have liked a little more discussion about combining information from digests produced by different enzymes, but no book can cover everything.

The last three chapters extend the discussion of analysis, working upwards from fragments to complete protein sequences. The three chapters respectively address three topics: using standard internet databases for recognizing fragments of known proteins, using combinations of strategies to analyze novel proteins, and using mass spec to identify post-translational modifications. That last one suffers from brevity; perhaps it was only meant to define a problem that deserves a whole book of its own.

Despite its throughness, the authors resist the urge for boggling detail. They present detail up to the point needed for understanding the mechanism and meaning of their topics, then stop. Lots of other writing would benefit from that kind of restraint.

I came away from this book well-informed, and ready to address specific topics in greater detail. That was exactly what I wanted. I recommend this book very highly.

//wiredweird

1 of 1 people found the following review helpful:

5.0 out of 5 stars Protein Sequencing and Identification Using Tandem Mass Spectrometry , May 18, 2007

By	Leonardo G. Silva "cogumelo.rol" (Campos, RJ-Brasil) - See all my reviews (REAL NAME)

Ao ler esse livro pude perceber que existe variadas técnicas que auxiliam na interpretação dos espectros de massas. A obra descreve os métodos de ionização e a formação dos íons moleculares e quasi-moleculares em cada um desses métodos. O livro tras a interpretação da distribuição de isótopos nas amostras, a identificação dos "parent ions", "base piks" e a distribuição das cargas durante a interpretação dos espectros. A interpretação de sequências de aminoácidos durante a análise de peptídios é rica em dicas, tabelas e exercícios resolvidos. De forma clara é possível entender a lógica por tras da formação das séries de íons A, B, C, X, Y e Z tão importantes durante a identificação da estrutura primária dos peptídeos. Se você pretende trabalhar com a identidficação de compostos de origem protéica utilizando espectrometria de massas, esse deverá ser seu livro de cabeceira!

5 of 9 people found the following review helpful:

5.0 out of 5 stars "Protein Sequencing ......" a must to read, October 26, 2000

By	BIG Mike Rose PHD (Crozet, Va.) - See all my reviews

Michael Kinter has presented the topic in a scientific yet enjoyable format. I found the information to be extremely interesting and beneficial in my laboratory. Lets face it folks this isn't the easiest nor most interesting topic to write about. Job well done DR. Kinter. I only have one criticism, there should have been more photos and illustrations. Get your copies quick this will no doubt be on the NewYork times best seller list before long.