This volume provides a set of protocols for the analysis of cloned genes. A set of techniques is described for the basic manipulation and mutagenesis of the cloned sequences. The rest of the volume is organized into a collection of techniques appropriate to the analytical route to be investigated; these include genome structure, sequence variation, gene expression, and protein function. Finally, a number of methods are described to take the step from the first gene to those with which it interacts.
Protocols for Gene Analysis (Methods in Molecular Biology)
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...recommended...A notes section that follows each methods section may be the most useful feature. These notes are like having the author right there explaining why certain things are done or not done, what variations may be tolerated or useful, what might go wrong, and how to remedy it. -Biopharm Manufacturing
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Inside This Book
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First Sentence:
The use of an electrical field to reversibly permeabilize cells (electroporation) has become a valuable technique for transfer of DNA into both eukaryotic and prokaryotic cells. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
pulse time ramps, gel support film, lac transcription, common oligonucleotide, biotin phosphoramidite, mutagenic oligonucleotide, clonality assay, sonication buffer, nuclear protein extract, band inversion, plaque screening, mutant oligonucleotide, terminal oligonucleotides, probe buffer, designed mutation, gel strip, polynucleotide kinase buffer, ligase buffer, mutagenic primer, ligation buffer, genomic footprinting, melting temperature agarose, annealing buffer, terminal transferase activity, stop mix Key Phrases - Capitalized Phrases (CAPs): (learn more)
Nucleic Acids Res, Harwood Copyright, Humana Press Inc, Cold Spring Harbor Laboratory, New York, Laboratory Manual, Arlington Heights, Practical Approach, Materials All, New England Biolabs, Applied Biosystems, Cell Biol, Foster City, Preparation of Nuclei, Cancer Res, Durrant Fig, Karsten Henco, Life Sci New!
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Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
The use of an electrical field to reversibly permeabilize cells (electroporation) has become a valuable technique for transfer of DNA into both eukaryotic and prokaryotic cells. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
pulse time ramps, gel support film, lac transcription, common oligonucleotide, biotin phosphoramidite, mutagenic oligonucleotide, clonality assay, sonication buffer, nuclear protein extract, band inversion, plaque screening, mutant oligonucleotide, terminal oligonucleotides, probe buffer, designed mutation, gel strip, polynucleotide kinase buffer, ligase buffer, mutagenic primer, ligation buffer, genomic footprinting, melting temperature agarose, annealing buffer, terminal transferase activity, stop mix Key Phrases - Capitalized Phrases (CAPs): (learn more)
Nucleic Acids Res, Harwood Copyright, Humana Press Inc, Cold Spring Harbor Laboratory, New York, Laboratory Manual, Arlington Heights, Practical Approach, Materials All, New England Biolabs, Applied Biosystems, Cell Biol, Foster City, Preparation of Nuclei, Cancer Res, Durrant Fig, Karsten Henco, Life Sci New!
Books on Related Topics | Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Citations (learn more)
This book cites 30
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44
books
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- Molecular Cloning: A Laboratory Manual, Third Edition (3 volume set) by Joe Sambrook on 9 pages
- BIOS Instant Notes in Molecular Biology by Phil Turner in Front Matter, and page 323
- Basic Methods Molecular Biolgy by Davis on page 124, and page 287
- Laboratory Manual by Nigel Cook on page 87
- Immunological Methods by Ivan Lefkovits on page 399
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- Gel Electrophoresis: Nucleic Acids (Introduction to Biotechniques) by Dr Robin Martin on 4 pages
- Yac Protocols (Methods in Molecular Biology) by David Markie in Front Matter, and Back Matter
- Plant Cell Electroporation And Electrofusion Protocols (Methods in Molecular Biology) by Jac A. Nickoloff in Front Matter, and Back Matter
- Animal Cell Electroporation and Electrofusion Protocols (Methods in Molecular Biology) by Jac A. Nickoloff in Front Matter, and Back Matter
- Species Diagnostics Protocols: Pcr and Other Nucleic Acid Methods (Methods in Molecular Biology) by Justin P. Clapp in Front Matter, and Back Matter
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