This book provides an exceptionally useful collection of tried and tested protocols for nucleic acid analysis that avoid the use of radioisotopes. Southern (i.e., DNA) and Northern (i.e., RNA) blotting protocols are covered in detail, from how to isolate good quality nucleic acids from plant and animal sources, to the use of the widest range of probe systems (involving digoxigenin, biotin, fluorescein, horseradish peroxidase labeling, and colorimetric and chemiluminescent detection). Other techniques treated in detail include hybridization in situ to chromosomes and transcribed RNA, newer and less conventional technologies, such as Randomly Amplified Polymorphic DNA (RAPD), reverse dot blots, bacterial identification using magnetic bead and dipstick assays, and nucleic acid sequence-based amplification.
Protocols for Nucleic Acid Analysis by Nonradioactive Probes
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Editorial Reviews
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...this book provides a collection of good protocols which are in general clearly written. They can be easily employed on the bench without being a specialist in a particular fields. - FEBS Letters
...Covers the basics of a variety of probe-labelling systems very well...a very interesting read. It could save you money and trouble from the radiation safety officer.-Biotechnology and Applied Biochemistry
...Covers the basics of a variety of probe-labelling systems very well...a very interesting read. It could save you money and trouble from the radiation safety officer.-Biotechnology and Applied Biochemistry
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Inside This Book
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First Sentence:
It is the aim of this book to provide working, reliable, protocols for nucleic acid analysis that avoid the use of radioisotopes. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
probe diluent, secondary wash buffer, squashing method, high target applications, primary wash buffer, column wash buffer, nonradioactive systems, dropping method, nucleic acid analysis, digoxigenin probes, optimum exposure time, plaque screening, plastic cover slip, gel tray, probe reagent, situ hybridization signal, detection buffer, hybridization buffer, posthybridization washes, nonradioactive probes, stringent wash, sterile distilled water, deionized formamide, probe concentration, labeling reaction Key Phrases - Capitalized Phrases (CAPs): (learn more)
Humana Press Inc, Nonradioactive Probes Edited, Nucleic Acids Res, Amersham International, Saran Wrap, Boehringer Mannheim, San Diego, New York, Pall Biodyne, Life Sci, Millipore Milliblot, Difco Laboratories, Dow Chemical Company, Materials All, Competitive Grants Program, Laboratory Manual, Plant Mol, Practical Approach, United Kingdom New!
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Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
It is the aim of this book to provide working, reliable, protocols for nucleic acid analysis that avoid the use of radioisotopes. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
probe diluent, secondary wash buffer, squashing method, high target applications, primary wash buffer, column wash buffer, nonradioactive systems, dropping method, nucleic acid analysis, digoxigenin probes, optimum exposure time, plaque screening, plastic cover slip, gel tray, probe reagent, situ hybridization signal, detection buffer, hybridization buffer, posthybridization washes, nonradioactive probes, stringent wash, sterile distilled water, deionized formamide, probe concentration, labeling reaction Key Phrases - Capitalized Phrases (CAPs): (learn more)
Humana Press Inc, Nonradioactive Probes Edited, Nucleic Acids Res, Amersham International, Saran Wrap, Boehringer Mannheim, San Diego, New York, Pall Biodyne, Life Sci, Millipore Milliblot, Difco Laboratories, Dow Chemical Company, Materials All, Competitive Grants Program, Laboratory Manual, Plant Mol, Practical Approach, United Kingdom New!
Books on Related Topics | Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Citations (learn more)
This book cites 18
books:
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46
books
cite this book:
See all 46 books citing this book
- Plant Genetic Transformation and Gene Expression: A Laboratory Manual by John Draper on page 40
- BIOS Instant Notes in Molecular Biology by Phil Turner in Front Matter
- Pathology of the Nucleus (Current Topics in Pathology) by J. C. E. Underwood on page 191
- Molecular Parasitology by John E. Hyde in Front Matter
- Glycoprotein Analysis in Biomedicine (Methods in Molecular Biology) by Elizabeth F. Hounsell in Front Matter
See all 18 books this book cites
- Species Diagnostics Protocols: Pcr and Other Nucleic Acid Methods (Methods in Molecular Biology) by Justin P. Clapp in Front Matter, page 225, and Back Matter
- Yac Protocols (Methods in Molecular Biology) by David Markie in Front Matter, and Back Matter
- Antibody Engineering Protocols (Methods in Molecular Biology) by Sudhir Paul in Front Matter, and Back Matter
- Artificial DNA: Methods and Applications by Yury E. Khudyakov on page 148, and page 151
- Animal Cell Electroporation and Electrofusion Protocols (Methods in Molecular Biology) by Jac A. Nickoloff in Front Matter, and Back Matter
See all 46 books citing this book
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