DNA Repair Protocols, Eukaryotic Systems (Methods in Molecular Biology) [Hardcover]

Daryl S. Henderson (Editor)

Book Description

Publication Date: July 15, 1999 | ISBN-10: 0896038025 | ISBN-13: 978-0896038028 | Edition: 1st

Daryl S. Henderson and a team of hands-on experts give time-tested instructions for analyzing a wide range of DNA repair processes and cellular responses to DNA damage, including nucleotide and base excision repair, DNA strand break repair, and mismatch repair. The methods focus on eukaryotic model systems that have made, or have the potential to make, important contributions to our understanding of cellular responses to DNA damage in relation to mutagenesis, carcinogenesis, and the cell cycle. Although mammalian cells predominate, consideration is also given to such important nonmammalian model systems as yeast, C. elegans (nematode), Drosophila (fruitfly), Xenopus (amphibian), and plants. DNA Repair Protocols: Eukaryotic Systems offers the most comprehensive collection of DNA repair protocols available, all step-by-step, optimized, and eminently reproducible with tips. It will serve as the gold-standard reference for both the practical and theoretical aspects of DNA repair studies, encourage the transfer of methodologies between model systems, and stimulate the development of new approaches.

Editorial Reviews

Review

"...a comprehensive series of technique-oriented chapters focusing on eukaryotic DNA repair methodology. ...this text succeeds admirably...The scope of the text is fairly broad and encompasses not only in vitro biochemistry and enzymology, but also cell biology and genetics and even signal transduction....In vitro biochemical assays are well covered,...A particularly nice feature of the book is that the chapters have undergone uniform editing and formatting. All provide a nice overview of the topic, including a brief review of the pertinent literature, followed by step-by-step methods sections. The methods are clearly presented in annotated outline form with cross referencing and high level of detail. Each chapter has a particularly valuable section at the end, called "Notes" in which the authors present some of the nitty-gritty details and tricks of the trade needed to make the techniques work....Overall, this book should provide a valuable laboratory companion for researchers in the area of DNA repair. It serves to provide useful and readable introductions to various topics, along with techniques protocols sufficient for reproducibility....this text will have substantial appeal to the readers of Radiation Research."-Radiation Research "The list of authors contains many of the leading scientists within the field...Protocols for most experimental eukaryotic organisms are described, from yeast through plants, worms, flies and frogs to mammals. Another laudable quality of this book is the standardization of the descriptions in Materials and methods. Since (almost) all articles are organized similarly, it is relatively easy to find what you want. Also, technical details have been standardized....At the end of each article , there is a 'Notes' section with detailed explanation of specific technical points. For a novice it is good to be reminded that ethidium bromide is a mutagen and that lids should be loosened before putting flasks in the microwave oven. I particularly liked the description, written by the editor, of how to squash Drosophila larvae on a microscope slide by"standing on it with the ball of your foot or your heel. If using the foot method, place the slide (sandwiched in 3 MED MER) on a hard clean floor, cover it carefully with a piece of wood, and stand on that". ...this book keeps up the reputation of the 'Methods in Molecular Biology' series and I would recommend if for labs working with DNA repair, in particular for use by students and technicians."-FEBS Letters

From the Back Cover

In DNA Repair Protocols: Eukaryotic Systems, Daryl S. Henderson and a team of hands-on experts give time-tested instructions for analyzing a wide range of DNA repair processes and cellular responses to DNA damage, including nucleotide and base excision repair, DNA strand break repair, and mismatch repair. The methods focus on eukaryotic model systems that have made, or have the potential to make, important contributions to our understanding of cellular responses to DNA damage in relation to mutagenesis, carcinogenesis, and the cell cycle. Although mammalian cells predominate, consideration is also given to such important nonmammalian model systems as yeast, C. elegans (nematode), Drosophila (fruitfly), Xenopus (amphibian), and plants. DNA Repair Protocols: Eukaryotic Systems offers the most comprehensive collection of DNA repair protocols available, all step-by-step, optimized, and eminently reproducible with tips on troubleshooting and avoiding pitfalls. It will serve as the gold-standard reference for both the practical and theoretical aspects of DNA repair studies, encourage the transfer of methodologies between model systems, and stimulate the development of new approaches.

Product Details

• Hardcover: 641 pages
• Publisher: Humana Press; 1st edition (July 15, 1999)
• Language: English
• ISBN-10: 0896038025
• ISBN-13: 978-0896038028
• Product Dimensions: 9.1 x 6.2 x 1.4 inches
• Shipping Weight: 2.4 pounds (View shipping rates and policies)
• Amazon Best Sellers Rank: #4,260,768 in Books (See Top 100 in Books)