Experts present methods and protocols essential for understanding parasites at the molecular level. The protocols cover culturing techniques for the major experimental organisms, methods for isolating and processing nucleic acids and proteins, PCR-based protocols for parasite identification, gene isolation and mutation, antibody-based procedures, chromosome and epitope mapping, flow cytometry, RNA sequencing, and parasite transformation.
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...well-written and thoughtfully assembled...will be welcomed by recent arrivals...and regularly utilized by instructors of future investigators. - Analytical Biochemistry
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Inside This Book
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First Sentence:
Any biochemical analysis is usually made easier by the availability of large numbers of cells to be analyzed, and one of the reasons for the position held by Trypanosoma brucei as the best characterized parasite is the relative ease with which it can be cultured in the laboratory. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
exonuclease protocol, mutation specific primer, leishmanial promastigotes, gametocyte cultures, parasitized blood, procyclic trypanosomes, defibrinated rabbit blood, touch blots, polyspecific antisera, colorimetry methods, form trypomastigotes, diagnostic primer, merozoite surface antigen, blotting sandwich, cruzi strains, parasite material, eluted antibody, mature gametocytes, pyrimethamine resistance, metacyclic trypomastigotes, postembedding labeling, isozyme variability, bloodstream trypanosomes, buffer codes, form trypanosomes Key Phrases - Capitalized Phrases (CAPs): (learn more)
Acids Res, Cold Spring Harbor, Humana Press Inc, Hyde Copyright, Molecular Parasitology Edited, New York, Laboratory Manual, Boehringer Mannheim, Arlington Heights, San Diego, Perkin Elmer, World Health Organization, Applied Biosystems, Cell Biol, Gene Pulser, John Wiley, Oswaldo Cruz, Rio de Janeiro, Soaking Migration Voltage, Tropical Diseases, Detection of Plasmodium, Fast Blue, Genetic Engineering, Methods Enzymol, Parasite Immunol New!
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Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Any biochemical analysis is usually made easier by the availability of large numbers of cells to be analyzed, and one of the reasons for the position held by Trypanosoma brucei as the best characterized parasite is the relative ease with which it can be cultured in the laboratory. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
exonuclease protocol, mutation specific primer, leishmanial promastigotes, gametocyte cultures, parasitized blood, procyclic trypanosomes, defibrinated rabbit blood, touch blots, polyspecific antisera, colorimetry methods, form trypomastigotes, diagnostic primer, merozoite surface antigen, blotting sandwich, cruzi strains, parasite material, eluted antibody, mature gametocytes, pyrimethamine resistance, metacyclic trypomastigotes, postembedding labeling, isozyme variability, bloodstream trypanosomes, buffer codes, form trypanosomes Key Phrases - Capitalized Phrases (CAPs): (learn more)
Acids Res, Cold Spring Harbor, Humana Press Inc, Hyde Copyright, Molecular Parasitology Edited, New York, Laboratory Manual, Boehringer Mannheim, Arlington Heights, San Diego, Perkin Elmer, World Health Organization, Applied Biosystems, Cell Biol, Gene Pulser, John Wiley, Oswaldo Cruz, Rio de Janeiro, Soaking Migration Voltage, Tropical Diseases, Detection of Plasmodium, Fast Blue, Genetic Engineering, Methods Enzymol, Parasite Immunol New!
Books on Related Topics | Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Citations (learn more)
This book cites 34
books:
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1
book
cites this book:
- Molecular Parasitology by John E. Hyde on 37 pages
- Molecular Cloning: A Laboratory Manual, Third Edition (3 volume set) by Joe Sambrook on 10 pages
- Antibodies by Edward Harlow on page 88, page 301, and page 406
- Immunological Methods by Ivan Lefkovits in Back Matter (1), and Back Matter (2)
- In Vitro Methods for Parasite Cultivation by A. E. R. Taylor on page 27, and page 40
See all 34 books this book cites
- Functional Microbial Genomics, Volume 33 (Methods in Microbiology) by Brendan Wren on page 5
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