This book is a comprehensive manual to allow both the novice researcher and the expert to set up and carry out quantitative PCR assays from scratch. However, this book also sets out to explain as many features of qPCR as possible, provide alternative viewpoints, methods, and aims to simulate the researchers into generating, interpreting, and publishing data that are reproducible, reliable, and biologically meaningful
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Editorial Reviews
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A book that should be a must read for anyone wanting to get into the field of real-time quantitative PCR -- Gregory L. Shipley, Ph. D., Director, Quantitative Genomics Core Laboratory, Department of Integrative Biology and Pharmacology, The University of Texas Health Science Center-Houston
This book will quickly become an essential reference manual for every lab wanting to perform quantitative PCR -- David G. Ginzinger, Ph. D., Laboratory Director, Genome Analysis Core, UCSF Comprehensive Cancer Center
This book will quickly become an essential reference manual for every lab wanting to perform quantitative PCR -- David G. Ginzinger, Ph. D., Laboratory Director, Genome Analysis Core, UCSF Comprehensive Cancer Center
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1 of 3 people found the following review helpful:
4.0 out of 5 stars
qPCR methods,
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This review is from: A-Z of Quantitative PCR (IUL Biotechnology, No. 5) (Hardcover)
Book arrived promptly and in great shape. Disappointed there was not coverage of one-step qPCR.
0 of 2 people found the following review helpful:
4.0 out of 5 stars
Real Time PCR bible,
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This review is from: A-Z of Quantitative PCR (IUL Biotechnology, No. 5) (Hardcover)
This book fills a large gap in the literature. I wish there were more like that.
Jonas Blomberg
0 of 2 people found the following review helpful:
5.0 out of 5 stars
Reference book,
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This review is from: A-Z of Quantitative PCR (IUL Biotechnology, No. 5) (Hardcover)
This is a very good reference book for my Molecular Lab. Every Molecular lab should have this book.
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Inside This Book
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First Sentence:
Perhaps the most irritating thing about the polymerase chain reaction (PCR) is its simplicity: Many of us still cannot understand why we were not the ones to think of it first. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
quantitative genotyping, spectral crosstalk, passive reference dye, dark quenchers, nonspecific chemistries, melting curve analysis, amplification plot, melting curve profiles, molecular beacons, core reagent kits, blastomere isolation, reporter fluorophore, quantification strategies, standardized mixtures, native template, scorpion primers, amplification efficiency, low copy number targets, reference gene, reaction master mix, vivo biopsies, multiplex reaction, threshold cycle, gene expression measurement, detection chemistry Key Phrases - Capitalized Phrases (CAPs): (learn more)
Nucleic Acids Res, Anal Biochem, Applied Biosystems, Clin Chem, Clin Microbiol, Proc Natl Acad Sci, United Kingdom, Cancer Res, Mol Cell Probes, Methods Appl, Biochem Biophys Res Commun, Biochim Biophys Acta, Color Plates, Mol Diagn, Biol Chem, Nat Biotechnol, The Netherlands, Cell Biol, Genome Res, Mol Biotechnol, Molecular Probes, Primer Express, University of London, Virol Methods, Foster City New!
Books on Related Topics | Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Perhaps the most irritating thing about the polymerase chain reaction (PCR) is its simplicity: Many of us still cannot understand why we were not the ones to think of it first. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
quantitative genotyping, spectral crosstalk, passive reference dye, dark quenchers, nonspecific chemistries, melting curve analysis, amplification plot, melting curve profiles, molecular beacons, core reagent kits, blastomere isolation, reporter fluorophore, quantification strategies, standardized mixtures, native template, scorpion primers, amplification efficiency, low copy number targets, reference gene, reaction master mix, vivo biopsies, multiplex reaction, threshold cycle, gene expression measurement, detection chemistry Key Phrases - Capitalized Phrases (CAPs): (learn more)
Nucleic Acids Res, Anal Biochem, Applied Biosystems, Clin Chem, Clin Microbiol, Proc Natl Acad Sci, United Kingdom, Cancer Res, Mol Cell Probes, Methods Appl, Biochem Biophys Res Commun, Biochim Biophys Acta, Color Plates, Mol Diagn, Biol Chem, Nat Biotechnol, The Netherlands, Cell Biol, Genome Res, Mol Biotechnol, Molecular Probes, Primer Express, University of London, Virol Methods, Foster City New!
Books on Related Topics | Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Citations (learn more)
This book cites 9
books:
See all 9 books this book cites
- Protein Crystallization (Iul Biotechnology Series) by Terese M. Bergfors in Front Matter
- Stem Cells (Handbook of Experimental Pharmacology) by Anna M. Wobus on page 716
- Clinical Chemistry, 5e by William J. Marshall on page 688
- Molecular Biology by Robert F. Weaver on page 32
- Handbook of Parametric and Nonparametric Statistical Procedures: Third Edition by David J. Sheskin on page 120
See all 9 books this book cites
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